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BCKDK Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS626612

The second major step in the catabolism of the branched-chain amino acids, isoleucine, leucine, and valine, is irreversibly catalyzed by the branched-chain alpha-keto acid dehydrogenase complex (BCKD), an inner-mitochondrial enzyme complex composed of 3… More details

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Product Overview

Online Datasheet in PDF
Product Name
BCKDK, Polyclonal Antibody
Full Product Name
BCKDK, ID (3-methyl-2-oxobutanoate Dehydrogenase Lipoamide Kinase, Mitochondrial, Branched-chain alpha-ketoacid Dehydrogenase Kinase, BCKDHKIN, BCKD-kinase)
Product Gene Name
anti-BCKDK antibody
Product Synonym Names
Anti -BCKDK, ID (3-methyl-2-oxobutanoate Dehydrogenase Lipoamide Kinase, Mitochondrial, Branched-chain alpha-ketoacid Dehydrogenase Kinase, BCKDHKIN, BCKD-kinase)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human BCKDK.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
46,360 Da
3D Structure
ModBase 3D Structure for O14874
Immunogen
Synthetic peptide selected from the central region of human BCKDK (KLH).

Product Categories/Family for anti-BCKDK antibody

Antibodies; Abs to Protein Kinase Substrates
Applications Tested/Suitable for anti-BCKDK antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-BCKDK antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100

Western Blot (WB) of anti-BCKDK antibody

Western blot analysis of BCKDK (arrow) using rabbit polyclonal BCKDK Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BCKDK gene (Lane 2) (Origene Technologies).
Rabbit Anti-BCKDK Polyclonal Antibody – Cat# MBS626612

Immunohistochemistry (IHC) of anti-BCKDK antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-BCKDK Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS626612

Other Notes

Small volumes of anti-BCKDK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BCKDK purchase

MBS626612 is an antibody targeting BCKDK. Also known as BCKDK, ID (3-methyl-2-oxobutanoate Dehydrogenase Lipoamide Kinase, Mitochondrial, Branched-chain alpha-ketoacid Dehydrogenase Kinase, BCKDHKIN, BCKD-kinase). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BCKDK. It may not necessarily be applicable to this product.

NCBI GI #
49457139
UniProt Primary Accession #
O14874
UniProt Secondary Accession #
Q6FGL4; Q96IN5
NCBI Official Full Name
BCKDK
UniProt Protein Name
[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial
UniProt Synonym Protein Names
Branched-chain alpha-ketoacid dehydrogenase kinase
Protein Family
[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase
UniProt Gene Name
BCKDK
UniProt Entry Name
BCKD_HUMAN

UniProt Comments for BCKDK

BCKDK: an atypical protein kinase associated with the mitochondrial matrix. Contains a HATPase_c domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. Unlike PHKs, BCK dimerizes through direct interaction of two opposing nucleotide-binding domains. Phosphorylates and inactivates the E1-alpha subunit of the branched-chain ketoacid dehydrogenase (BCKDK), the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Inactivating mutations have been discovered in BCKDK in families with autism, epilepsy, and intellectual disability, a syndrome that may respond to dietary supplementation.

Protein type: Protein kinase, atypical; Kinase, protein; EC 2.7.11.4; Mitochondrial; ATYPICAL group; PDHK family

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: mitochondrion; mitochondrial alpha-ketoglutarate dehydrogenase complex

Molecular Function: protein serine/threonine kinase activity; [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] kinase activity; protein binding; kinase activity; ATP binding

Biological Process: branched chain family amino acid catabolic process; phosphorylation; protein amino acid phosphorylation; amino acid catabolic process

Disease: Branched-chain Ketoacid Dehydrogenase Kinase Deficiency
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BCKDK antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with BCKDK and FLNA

Pathways associated with anti-BCKDK antibody

 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram

Organs/Tissues associated with anti-BCKDK antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >2 publications with BCKDK and Liver
 Muscle Antibodies  >1 publications with BCKDK and Muscle
 Kidney Antibodies  >1 publications with BCKDK and Kidney
 Brain Antibodies  >1 publications with BCKDK and Brain
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