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BCL6 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758037

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
BCL6, Polyclonal Antibody
Full Product Name
Anti-BCL6 Antibody Picoband
Product Gene Name
anti-BCL6 antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
78,846 Da
Chromosome Location
Chromosome: 3; NC_000003.11 (187439165..187463513, complement). Location: 3q27
OMIM
109565
3D Structure
ModBase 3D Structure for P41182
Immunogen
E coli-derived human BCL6 recombinant protein (Position: R28-K336). Human BCL6 shares 90.6% amino acid (aa) sequence identity with mouse BCL6.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.

Product Categories/Family for anti-BCL6 antibody

Primary Antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-BCL6 antibody
Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-BCL6 antibody
WB: 0.25-0.5ug/ml, Human
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-BCL6 antibody

Figure 1. Western blot analysis of BCL6 using anti-BCL6 antibody (MBS1758037).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human Daudi whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL6 antigen affinity purified polyclonal antibody (#MBS1758037) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for BCL6 at approximately 88 kDa. The expected band size for BCL6 is at 79 kDa.
Rabbit Anti-BCL6 Polyclonal Antibody – Cat# MBS1758037

Flow Cytometry (FC/FACS) of anti-BCL6 antibody

Figure 2. Flow Cytometry analysis of Raji cells using anti-BCL6 antibody (MBS1758037).
Overlay histogram showing Raji cells stained with MBS1758037 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCL6 Antibody (MBS1758037, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Rabbit Anti-BCL6 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758037

Product References and Citations for anti-BCL6 antibody

1. Akasaka, T., Miura, I., Takahashi, N., Akasaka, H., Yonetani, N., Ohno, H., Fukuhara, S., Okuma, M. A recurring translocation, t(3;6)(q27;p21), in non-Hodgkin's lymphoma results in replacement of the 5-prime regulatory region of BCL6 with a novel H4 histone gene. Cancer Res. 57: 7-12, 1997. 2. Barnett, B. E., Ciocca, M. L., Goenka, R., Barnett, L. G., Wu, J., Laufer, T. M., Burkhardt, J. K., Cancro, M. P., Reiner, S. L. Asymmetric B cell division in the germinal center reaction. Science 335: 342-344, 2012.3. Baron, B. W., Nucifora, G., McCabe, N., Espinosa, R., III, Le Beau, M. M., McKeithan, T. W. Identification of the gene associated with the recurring chromosomal translocations t(3;14)(q27;q32) and t(3;22)(q27;q11) in B-cell lymphomas. Proc. Nat. Acad. Sci. 90: 5262-5266, 1993.

Other Notes

Small volumes of anti-BCL6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BCL6 purchase

MBS1758037 is an antibody targeting BCL6. Also known as Anti-BCL6 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BCL6. It may not necessarily be applicable to this product.

NCBI GI #
195927002
NCBI GeneID
604
NCBI Accession #
NP_001124317.1
UniProt Primary Accession #
P41182
UniProt Secondary Accession #
A7E241; B8PSA7; D3DNV5
UniProt Related Accession #
P41182
GenBank Nucleotide #
NM_001130845.1
NCBI Official Full Name
B-cell lymphoma 6 protein isoform 1
NCBI Official Synonym Full Names
B-cell CLL/lymphoma 6
NCBI Official Symbol
BCL6
NCBI Official Synonym Symbols
BCL5; LAZ3; BCL6A; ZNF51; ZBTB27
UniProt Protein Name
B-cell lymphoma 6 protein
UniProt Synonym Protein Names
B-cell lymphoma 5 protein; BCL-5; Protein LAZ-3; Zinc finger and BTB domain-containing protein 27; Zinc finger protein 51
Protein Family
B-cell lymphoma 6 protein
UniProt Gene Name
BCL6
UniProt Synonym Gene Names
BCL5; LAZ3; ZBTB27; ZNF51; BCL-6; BCL-5
UniProt Entry Name
BCL6_HUMAN
NCBI Protein Information
B-cell lymphoma 6 protein; BCL-5; BCL-6; protein LAZ-3; zinc finger protein 51; B-cell lymphoma 5 protein; B-cell lymphoma 6 protein transcript; zinc finger transcription factor BCL6S; cys-his2 zinc finger transcription factor; zinc finger and BTB domain-

NCBI Summary for BCL6

The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
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UniProt Comments for BCL6

Bcl-6: a zinc finger transcription factor and contains an N-terminal BTB/POZ domain. A sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. Mediates transcriptional repression and interacts with components of histone deacetylase co-repressor complexes including N-CoR and SMRT. The gene is frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL.

Protein type: C2H2-type zinc finger protein; Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: replication fork; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; chromatin DNA binding; metal ion binding; chromatin binding; transcription factor activity

Biological Process: erythrocyte development; positive regulation of apoptosis; regulation of memory T cell differentiation; cell morphogenesis; negative regulation of transcription from RNA polymerase II promoter; Rho protein signal transduction; negative regulation of cell proliferation; negative regulation of isotype switching to IgE isotypes; positive regulation of histone deacetylation; positive regulation of B cell proliferation; germinal center formation; negative regulation of mast cell cytokine production; inflammatory response; negative regulation of T-helper 2 cell differentiation; regulation of immune response; T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of cell motility; negative regulation of cell-matrix adhesion; regulation of germinal center formation; negative regulation of Rho protein signal transduction; protein import into nucleus, translocation; B cell differentiation; regulation of inflammatory response; negative regulation of Notch signaling pathway; spermatogenesis; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; positive regulation of neuron differentiation; actin cytoskeleton organization and biogenesis; response to DNA damage stimulus; negative regulation of B cell apoptosis
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Research Articles on BCL6

1. No correlation was found between BCL-6 gene rearrangement and BCL-6 protein expression in diffuse large B-cell lymphoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BCL6 antibody

 Reference Product  PubMed Publications
 JUN antibody  >83 publications with BCL6 and JUN
 IRF4 antibody  >64 publications with BCL6 and IRF4
 BCOR antibody  >35 publications with BCL6 and BCOR
 TP53 antibody  >24 publications with BCL6 and TP53
 CCND2 antibody  >13 publications with BCL6 and CCND2
 BCL6B antibody  >10 publications with BCL6 and BCL6B
 NCOR2 antibody  >9 publications with BCL6 and NCOR2
 ZBTB16 antibody  >7 publications with BCL6 and ZBTB16
 ZBTB7A antibody  >5 publications with BCL6 and ZBTB7A
 HDAC5 antibody  >4 publications with BCL6 and HDAC5

Pathways associated with anti-BCL6 antibody

 Products by Pathway  Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 FoxO Family Signaling Pathway antibodies  FoxO Family Signaling Pathway Diagram
 IL4-mediated Signaling Events Pathway antibodies  IL4-mediated Signaling Events Pathway Diagram
 Signaling Events Mediated By HDAC Class II Pathway antibodies  Signaling Events Mediated By HDAC Class II Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram

Diseases associated with anti-BCL6 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >677 publications with BCL6 and Neoplasms
 Lymphoma, T-Cell Antibodies  >91 publications with BCL6 and Lymphoma, T-Cell
 Neoplasms, Experimental Antibodies  >65 publications with BCL6 and Neoplasms, Experimental
 Cell Transformation, Neoplastic Antibodies  >46 publications with BCL6 and Cell Transformation, Neoplastic
 Inflammation Antibodies  >40 publications with BCL6 and Inflammation
 Multiple Myeloma Antibodies  >23 publications with BCL6 and Multiple Myeloma
 Carcinoma Antibodies  >21 publications with BCL6 and Carcinoma
 Breast Neoplasms Antibodies  >20 publications with BCL6 and Breast Neoplasms
 Hyperplasia Antibodies  >18 publications with BCL6 and Hyperplasia
 Necrosis Antibodies  >12 publications with BCL6 and Necrosis

Organs/Tissues associated with anti-BCL6 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >414 publications with BCL6 and Blood
 Lymph Node Antibodies  >241 publications with BCL6 and Lymph Node
 Lymph Antibodies  >82 publications with BCL6 and Lymph
 Bone Antibodies  >70 publications with BCL6 and Bone
 Skin Antibodies  >31 publications with BCL6 and Skin
 Spleen Antibodies  >30 publications with BCL6 and Spleen
 Brain Antibodies  >23 publications with BCL6 and Brain
 Liver Antibodies  >20 publications with BCL6 and Liver
 Stomach Antibodies  >16 publications with BCL6 and Stomach
 Muscle Antibodies  >16 publications with BCL6 and Muscle
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