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BGN Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6465271

MBS6465271 is an antibody targeting BGN. Also known as BGN, ID (Biglycan, Bone/Cartilage Proteoglycan I, PG-S1, SLRR1A) (MaxLight 650). Reactive in Human. Host: Rabbit. More details

$1060 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BGN, Polyclonal Antibody
Full Product Name
BGN, ID (Biglycan, Bone/Cartilage Proteoglycan I, PG-S1, SLRR1A) (MaxLight 650)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human BGN.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
39.5 kDa (352aa)
OMIM
301870
3D Structure
ModBase 3D Structure for P21810
Immunogen
Synthetic peptide selected from the center region of human BGN (KLH).
Conjugate
MaxLight650
Note
Preservative Free
Applications Tested/Suitable for anti-BGN antibody
Western Blot (WB), FLISA
Application Notes for anti-BGN antibody
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-BGN antibody

Western blot analysis of BGN (arrow) using rabbit polyclonal BGN Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BGN gene.
Rabbit Anti-BGN Polyclonal Antibody – Cat# MBS6465271

Western Blot (WB) of anti-BGN antibody

Western blot analysis of BGN Antibody (Center) in Sk-Br-3 cell line lysates (35ug/lane). BGN (arrow) was detected using the purified Pab.
Rabbit Anti-BGN Polyclonal Antibody, Western Blot (WB) – Cat# MBS6465271

Immunohistochemistry (IHC) of anti-BGN antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with BGN Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-BGN Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6465271

Flow Cytometry (FC/FACS) of anti-BGN antibody

BGN Antibody (Center) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-BGN Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6465271

Product References and Citations for anti-BGN antibody

1. Nanao, K., Genomics 19(1), 27-30 (1994).

Other Notes

Small volumes of anti-BGN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BGN. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
biglycan preproprotein
NCBI Official Synonym Full Names
biglycan
NCBI Official Symbol
BGN
NCBI Official Synonym Symbols
PGI; MRLS; DSPG1; PG-S1; SEMDX; SLRR1A
UniProt Protein Name
Biglycan
UniProt Synonym Protein Names
Bone/cartilage proteoglycan I; PG-S1
Protein Family
UniProt Gene Name
BGN
UniProt Synonym Gene Names
SLRR1A
UniProt Entry Name
PGS1_HUMAN
NCBI Protein Information
biglycan

NCBI Summary for BGN

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in bone growth, muscle development and regeneration, and collagen fibril assembly in multiple tissues. This protein may also regulate inflammation and innate immunity. Additionally, the encoded protein may contribute to atherosclerosis and aortic valve stenosis in human patients. This gene and the related gene decorin are thought to be the result of a gene duplication. [provided by RefSeq, Nov 2015]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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