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BID Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1751271

BID (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ… More details

$630 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Bid, Polyclonal Antibody
Full Product Name
Anti-Human Bid DyLight 488 conjugated Antibody
Product Gene Name
anti-Bid antibody
Product Synonym Names
Rabbit IgG Polyclonal Anti-Human Bid Antibody DyLight 488 Conjugated, Flow Validated; BH3-interacting domain death agonist; p22 BID; BID; BH3-interacting domain death agonist p15; p15 BID; BH3-interacting domain death agonist p13; p13 BID; BH3-interacting domain death agonist p11; p11 BID; BID; BH3 interacting domain death agonist
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at 2-8 degree C for one year. Protect from light. Do not freeze.
Molecular Weight
26,836 Da
3D Structure
ModBase 3D Structure for P55957
Immunogen
E Coli-derived human Bid recombinant protein (Position: M1-D195). Human Bid shares 64% and 61% amino acid (aa) sequence identity with mouse and rat Bid, respectively.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Bid antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-Bid antibody
FC/FACS: 1-3ug/1x106 cells

Flow Cytometry (FC/FACS) of anti-Bid antibody

Figure 1. Flow Cytometry analysis of K562 cells using anti-Human Bid antibody.
Overlay histogram showing K562 cells stained with A00730-Dyl488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human Bid Antibody for 30 min at 20 degree C. DyLight® 488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-BID Polyclonal Antibody – Cat# MBS1751271

Product References and Citations for anti-Bid antibody

1. Chou, J. J., Li, H., Salvesen, G. S., Yuan, J., Wagner, G. Solution structure of BID, an intracellular amplifier of apoptotic signaling. Cell 95: 615-624, 1999. 2. Luo, X., Budihardjo, I., Zou, H., Slaughter, C., Wang, X. Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors. Cell 94: 481-490, 1998. 3. Wang, K., Yin, X.-M., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Keck, C. L., Zimonjic, D. B., Popescu, N. C., Korsmeyer, S. J. BID, a proapoptotic BCL-2 family member, is localized to mouse chromosome 6 and human chromosome 22q11. Genomics 53: 235-238, 1998.

Other Notes

Small volumes of anti-Bid antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Bid purchase

MBS1751271 is an antibody targeting Bid. Also known as Anti-Human Bid DyLight 488 conjugated Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Bid. It may not necessarily be applicable to this product.

NCBI GI #
4557361
NCBI GeneID
637
NCBI Accession #
NP_001187.1
UniProt Primary Accession #
P55957
UniProt Secondary Accession #
Q549M7; Q71T04; Q7Z4M9; Q8IY86
UniProt Related Accession #
P55957
GenBank Nucleotide #
NM_001196.3
NCBI Official Full Name
BH3-interacting domain death agonist isoform 2
NCBI Official Synonym Full Names
BH3 interacting domain death agonist
NCBI Official Symbol
BID
NCBI Official Synonym Symbols
FP497
UniProt Protein Name
BH3-interacting domain death agonist
UniProt Synonym Protein Names
p22 BID; BID
Protein Family
BH3-interacting domain death agonist
UniProt Gene Name
BID
UniProt Synonym Gene Names
BID
NCBI Protein Information
BH3-interacting domain death agonist

NCBI Summary for Bid

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]
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UniProt Comments for Bid

The major proteolytic product p15 BID allows the release of cytochrome c (). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.

Research Articles on Bid

1. results suggest that histone modifications in the promoter region of miR-500a may be responsible for the increased expression of miR-500a in Hepatocellular carcinoma (HCC), which promotes cancer progression by targeting BID, indicating that miR-500a may be a potential prognostic predictor and therapeutic target for HCC patients.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Bid antibody

 Reference Product  PubMed Publications
 CASP8 antibody  >1096 publications with Bid and CASP8
 BAX antibody  >1007 publications with Bid and BAX
 CASP3 antibody  >937 publications with Bid and CASP3
 BCL2L1 antibody  >326 publications with Bid and BCL2L1
 BAK1 antibody  >109 publications with Bid and BAK1
 BCL2 antibody  >91 publications with Bid and BCL2
 GZMB antibody  >41 publications with Bid and GZMB
 CASP10 antibody  >40 publications with Bid and CASP10
 CASP1 antibody  >23 publications with Bid and CASP1
 BCL2L2 antibody  >12 publications with Bid and BCL2L2

Pathways associated with anti-Bid antibody

 Products by Pathway  Pathway Diagram
 Activation And Oligomerization Of BAK Protein Pathway antibodies  Activation And Oligomerization Of BAK Protein Pathway Diagram
 Activation Of BAD And Translocation To Mitochondria Pathway antibodies  Activation Of BAD And Translocation To Mitochondria Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway antibodies  Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway Diagram
 Activation, Translocation And Oligomerization Of BAX Pathway antibodies  Activation, Translocation And Oligomerization Of BAX Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram

Diseases associated with anti-Bid antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1635 publications with Bid and Neoplasms
 Cardiovascular Diseases Antibodies  >797 publications with Bid and Cardiovascular Diseases
 Necrosis Antibodies  >647 publications with Bid and Necrosis
 Lung Diseases Antibodies  >616 publications with Bid and Lung Diseases
 Drug Toxicity Antibodies  >612 publications with Bid and Drug Toxicity
 Gastrointestinal Diseases Antibodies  >593 publications with Bid and Gastrointestinal Diseases
 Nervous System Diseases Antibodies  >546 publications with Bid and Nervous System Diseases
 Pain Antibodies  >414 publications with Bid and Pain
 Heart Diseases Antibodies  >393 publications with Bid and Heart Diseases
 Adenocarcinoma Antibodies  >308 publications with Bid and Adenocarcinoma

Organs/Tissues associated with anti-Bid antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1710 publications with Bid and Blood
 Liver Antibodies  >525 publications with Bid and Liver
 Heart Antibodies  >488 publications with Bid and Heart
 Lung Antibodies  >468 publications with Bid and Lung
 Brain Antibodies  >334 publications with Bid and Brain
 Vascular Antibodies  >293 publications with Bid and Vascular
 Kidney Antibodies  >248 publications with Bid and Kidney
 Skin Antibodies  >213 publications with Bid and Skin
 Bone Antibodies  >191 publications with Bid and Bone
 Stomach Antibodies  >164 publications with Bid and Stomach
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