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BLM Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6278484

BLM is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
BLM, Polyclonal Antibody
Full Product Name
BLM, CT (BLM, RECQ2, RECQL3, Bloom syndrome protein, DNA helicase, RecQ-like type 2, RecQ protein-like 3) (APC)
Product Gene Name
anti-BLM antibody
Product Synonym Names
BLM; RECQ2; RECQL3; Bloom syndrome protein; DNA helicase; RecQ-like type 2; RecQ protein-like 3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
159,000 Da
Chromosome Location
Chromosome: 15; NC_000015.9 (91260579..91358686). Location: 15q26.1
OMIM
210900
3D Structure
ModBase 3D Structure for P54132
Immunogen
BLM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1061~1090 amino acids from the C-terminal region of human BLM.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-BLM antibody

Antibodies; DNA Replication; Repair
Applications Tested/Suitable for anti-BLM antibody
Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
Application Notes for anti-BLM antibody
FC/FACS: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-BLM antibody

Western blot analysis of BLM Antibody (C-term) in HL-60 cell line lysates (35ug/lane). BLM (arrow) was detected using the purified Pab.
Rabbit Anti-BLM Polyclonal Antibody – Cat# MBS6278484

Flow Cytometry (FC/FACS) of anti-BLM antibody

BLM Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-BLM Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6278484

Product References and Citations for anti-BLM antibody

Davila, S., et al. Genes Immun. 11(3):232-238(2010). Frank, B., et al. Carcinogenesis 31(3):442-445(2010). Ouyang, K.J., et al. PLoS Biol. 7 (12), E1000252 (2009).

Other Notes

Small volumes of anti-BLM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BLM purchase

MBS6278484 is an antibody targeting BLM. Also known as BLM, CT (BLM, RECQ2, RECQL3, Bloom syndrome protein, DNA helicase, RecQ-like type 2, RecQ protein-like 3) (APC). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BLM. It may not necessarily be applicable to this product.

NCBI GI #
4557365
NCBI GeneID
641
NCBI Accession #
NP_000048.1
UniProt Primary Accession #
P54132
UniProt Secondary Accession #
Q52M96
UniProt Related Accession #
P54132
GenBank Nucleotide #
NM_000057.2
NCBI Official Full Name
Bloom syndrome protein
NCBI Official Synonym Full Names
Bloom syndrome, RecQ helicase-like
NCBI Official Symbol
BLM
NCBI Official Synonym Symbols
BS; RECQ2; RECQL2; RECQL3
UniProt Protein Name
Bloom syndrome protein
UniProt Synonym Protein Names
DNA helicase, RecQ-like type 2; RecQ2; RecQ protein-like 3
Protein Family
Bloom syndrome protein
UniProt Gene Name
BLM
UniProt Synonym Gene Names
RECQ2; RECQL3; RecQ2
UniProt Entry Name
BLM_HUMAN
NCBI Protein Information
Bloom syndrome protein; recQ protein-like 3; DNA helicase, RecQ-like type 2

NCBI Summary for BLM

The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq, Jul 2008]
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UniProt Comments for BLM

BLM: a magnesium-dependent ATP-dependent DNA-helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. A member of the RecQ helicase family that is required for genome stability. Participates in DNA replication, recombination and repair. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex which is a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ubiquitinated FANCD2.

Protein type: DNA repair, damage; DNA replication; Helicase; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: male germ cell nucleus; nuclear chromosome; lateral element; chromosome, telomeric region; PML body; nuclear matrix; pronucleus; cytoplasm; nucleolus; replication fork; nucleus

Molecular Function: four-way junction helicase activity; G-quadruplex DNA binding; ATP-dependent DNA helicase activity; protein binding; ATP-dependent 3'-5' DNA helicase activity; p53 binding; ATPase activity; ATP-dependent helicase activity; bubble DNA binding; helicase activity; single-stranded DNA binding; ATP binding

Biological Process: negative regulation of DNA recombination; regulation of binding; positive regulation of transcription, DNA-dependent; negative regulation of mitotic recombination; DNA double-strand break processing; alpha-beta T cell differentiation; negative regulation of cell division; DNA repair; double-strand break repair via homologous recombination; DNA duplex unwinding; protein oligomerization; DNA recombination; replication fork processing; mitotic cell cycle G2/M transition DNA damage checkpoint; DNA strand renaturation; positive regulation of alpha-beta T cell proliferation; replication fork protection; regulation of cyclin-dependent protein kinase activity; response to DNA damage stimulus; telomere maintenance; response to X-ray

Disease: Bloom Syndrome
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Research Articles on BLM

1. Eighteen polymorphisms in four DNA repair genes were genotyped in 789 age related cataract patients and 531 normal controls from the Jiangsu Eye Study.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BLM antibody

 Reference Product  PubMed Publications
 WRN antibody  >201 publications with BLM and WRN
 RAD51 antibody  >73 publications with BLM and RAD51
 CASP3 antibody  >15 publications with BLM and CASP3
 MLH1 antibody  >15 publications with BLM and MLH1
 RPA1 antibody  >13 publications with BLM and RPA1
 FANCD2 antibody  >12 publications with BLM and FANCD2
 FEN1 antibody  >10 publications with BLM and FEN1
 TP53 antibody  >10 publications with BLM and TP53
 TP53BP1 antibody  >9 publications with BLM and TP53BP1
 MX1 antibody  >2 publications with BLM and MX1

Pathways associated with anti-BLM antibody

 Products by Pathway  Pathway Diagram
 BRCA1-associated Genome Surveillance Complex (BASC) Pathway antibodies  BRCA1-associated Genome Surveillance Complex (BASC) Pathway Diagram
 Bloom's Syndrome Complex Pathway antibodies  Bloom's Syndrome Complex Pathway Diagram
 Fanconi Anemia Pathway antibodies  Fanconi Anemia Pathway Diagram
 Fanconi Anemia Pathway antibodies  Fanconi Anemia Pathway Diagram
 Homologous Recombination Pathway antibodies  Homologous Recombination Pathway Diagram
 Homologous Recombination Pathway antibodies  Homologous Recombination Pathway Diagram
 Integrated Breast Cancer Pathway antibodies  Integrated Breast Cancer Pathway Diagram
 Integrated Cancer Pathway antibodies  Integrated Cancer Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Meiosis Pathway antibodies  Meiosis Pathway Diagram

Diseases associated with anti-BLM antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >587 publications with BLM and Neoplasms
 Bloom Syndrome Antibodies  >422 publications with BLM and Bloom Syndrome
 Drug Toxicity Antibodies  >381 publications with BLM and Drug Toxicity
 Fibrosis Antibodies  >321 publications with BLM and Fibrosis
 Neoplasms, Experimental Antibodies  >139 publications with BLM and Neoplasms, Experimental
 Chromosome Aberrations Antibodies  >126 publications with BLM and Chromosome Aberrations
 Carcinoma, Squamous Cell Antibodies  >115 publications with BLM and Carcinoma, Squamous Cell
 Inflammation Antibodies  >95 publications with BLM and Inflammation
 Necrosis Antibodies  >79 publications with BLM and Necrosis
 Lung Neoplasms Antibodies  >59 publications with BLM and Lung Neoplasms

Organs/Tissues associated with anti-BLM antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >404 publications with BLM and Lung
 Blood Antibodies  >339 publications with BLM and Blood
 Kidney Antibodies  >166 publications with BLM and Kidney
 Liver Antibodies  >99 publications with BLM and Liver
 Intestine Antibodies  >92 publications with BLM and Intestine
 Muscle Antibodies  >83 publications with BLM and Muscle
 Skin Antibodies  >76 publications with BLM and Skin
 Lymph Node Antibodies  >58 publications with BLM and Lymph Node
 Brain Antibodies  >53 publications with BLM and Brain
 Spleen Antibodies  >37 publications with BLM and Spleen
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