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CAPZB Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8108059

MBS8108059 is an antibody targeting CAPZB. Also known as Anti-CAPZB Antibody, Rabbit Polyclonal. Reactive in Human. Host: Rabbit. More details

$370 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CAPZB, Polyclonal Antibody
Full Product Name
Anti-CAPZB Antibody, Rabbit Polyclonal
Product Gene Name
Product Synonym Names
CAPZB Antibody, Rabbit PAb, Antigen Affinity Purified; capping protein (actin filament) muscle Z-line, beta; Anti-CAPB Antibody; Anti-CAPPB Antibody; Anti-CAPZ Antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Purity/Purification
Protein A & Antigen Affinity
Specificity
Human CAPZB
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunogen
E Coli-derived Human CAPZB fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human CAPZB fragment, and purified by antigen affinity chromatography.
Applications Tested/Suitable for anti-CAPZB antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-CAPZB antibody
WB: 1:500-1:2000
IP: 1-5uL/mg of lysate

Western Blot (WB) of anti-CAPZB antibody

Anti-CAPZB rabbit polyclonal antibody at 1:500 dilution
Lane A: HeLa Whole Cell Lysate
Lane B: 293T Whole Cell Lysate
Lane C: Jurkat Whole Cell Lysate
Lane D: A431 Whole Cell Lysate
Lysates/proteins at 30 ug per lane.
Secondary
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:31 kDa
Observed band size:31 kDa
(We are unsure as to the identity of these extra bands.)
Rabbit Anti-CAPZB Polyclonal Antibody – Cat# MBS8108059

Immunoprecipitation (IP) of anti-CAPZB antibody

CAPZB was immunoprecipitated using:
Lane A:0.5 mg A431 Whole Cell Lysate
4 uL anti-CAPZB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.
Primary antibody:
Anti-CAPZB rabbit polyclonal antibody,at 1:100 dilution
Secondary antibody:
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size :31 kDa
Rabbit Anti-CAPZB Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS8108059

Other Notes

Small volumes of anti-CAPZB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CAPZB. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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