Rabbit Anti-CCNB1 Polyclonal Antibody – Cat# MBS6282124

Dot blot analysis of CCNB1 Antibody (Phospho S35) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

CCNB1 Antibody – Rabbit Polyclonal

Name: CCNB1 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6282124
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6282124

$1060 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CCNB1, Polyclonal Antibody

Full Product Name

CCNB1, Phosphorylated (S35) (CCNB1, CCNB, G2/mitotic-specific cyclin-B1) (AP)

Product Gene Name

anti-CCNB1 antibody

Product Synonym Names

CCNB1; CCNB; G2/mitotic-specific cyclin-B1

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

Purity/Purification

Purified by Protein A Affinity Chromatography.

Preparation and Storage

Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Molecular Weight

48,337 Da

Chromosome Location

Chromosome: 5; NC_000005.9 (68462837..68474072). Location: 5q12

OMIM

123836

3D Structure

ModBase 3D Structure for P14635

Immunogen

CCNB1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S35 of human CCNB1.

Conjugate

Antigen Modification

Phosphorylated

Note

Preservative Free

Product Categories/Family for anti-CCNB1 antibody

Antibodies; Cyclin

Applications Tested/Suitable for anti-CCNB1 antibody

ELISA (EIA), Dot Blot (DB)

Application Notes for anti-CCNB1 antibody

ELISA: 1:1,000
Applications are based on unconjugated antibody.

Product References and Citations for anti-CCNB1 antibody

Kreis, N.N., et al. Oncogene 29(41):5591-5603(2010). van Zon, W., et al. J. Cell Biol. 190(4):587-602(2010). Harley, M.E., et al. EMBO J. 29(14):2407-2420(2010). Olson, J.E., et al. Breast Cancer Res. Treat. (2010). In press: Nantajit, D., et al. PLoS ONE 5 (8), E12341 (2010).

Other Notes

Small volumes of anti-CCNB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CCNB1 purchase

MBS6282124 is an antibody targeting CCNB1. Also known as CCNB1, Phosphorylated (S35) (CCNB1, CCNB, G2/mitotic-specific cyclin-B1) (AP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CCNB1. It may not necessarily be applicable to this product.

NCBI GI #

14327896

NCBI GeneID

891

NCBI Accession #

NP_114172.1

UniProt Primary Accession #

P14635

UniProt Related Accession #

P14635

GenBank Nucleotide #

NM_031966.3

NCBI Official Full Name

G2/mitotic-specific cyclin-B1

NCBI Official Synonym Full Names

cyclin B1

NCBI Official Symbol

CCNB1

NCBI Official Synonym Symbols

CCNB

UniProt Protein Name

G2/mitotic-specific cyclin-B1

Protein Family

G2/mitotic-specific cyclin

UniProt Gene Name

CCNB1

UniProt Synonym Gene Names

CCNB

UniProt Entry Name

CCNB1_HUMAN

NCBI Protein Information

G2/mitotic-specific cyclin-B1; G2/mitotic-specific cyclin B1

NCBI Summary for CCNB1

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]

UniProt Comments for CCNB1

CCNB1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Complexes with cdc2 to form the maturation-promoting factor (MPF). Two alternatively spliced isoforms have been found, a constitutively expressed form and a cell cycle-regulated form that is expressed predominantly during G2/M phase.

Protein type: Activator; Cell cycle regulation

Chromosomal Location of Human Ortholog: 5q12

Cellular Component: nucleoplasm; spindle pole; centrosome; membrane; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; patched binding; histone kinase activity; protein kinase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of histone phosphorylation; regulation of cell cycle; ventricular cardiac muscle cell development; positive regulation of attachment of spindle microtubules to kinetochore; mitotic nuclear envelope disassembly; positive regulation of mRNA 3'-end processing; positive regulation of mitotic cell cycle; positive regulation of fibroblast proliferation; negative regulation of protein amino acid phosphorylation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; tissue regeneration; protein complex assembly; G2/M transition of mitotic cell cycle; positive regulation of cardiac muscle cell proliferation; response to drug; in utero embryonic development; oocyte maturation; mitotic metaphase plate congression; response to DDT; gut development; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; response to mechanical stimulus; cell division; spermatogenesis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; mitotic spindle stabilization; G1/S transition of mitotic cell cycle

Research Articles on CCNB1

1. Silencing beta-catenin gene may induce changes of cell cycle and in cyclin B1 and cyclin C protein expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CCNB1 antibody

Reference Product	PubMed Publications
CDK1 antibody	>436 publications with CCNB1 and CDK1
CDKN1A antibody	>281 publications with CCNB1 and CDKN1A
CDC25C antibody	>257 publications with CCNB1 and CDC25C
CDC25A antibody	>154 publications with CCNB1 and CDC25A
PCNA antibody	>150 publications with CCNB1 and PCNA
CDKN1B antibody	>120 publications with CCNB1 and CDKN1B
CDC20 antibody	>82 publications with CCNB1 and CDC20
PLK1 antibody	>79 publications with CCNB1 and PLK1
BRCA1 antibody	>30 publications with CCNB1 and BRCA1
FZR1 antibody	>17 publications with CCNB1 and FZR1

Pathways associated with anti-CCNB1 antibody

Products by Pathway	Pathway Diagram
AMPK Signaling Pathway antibodies	AMPK Signaling Pathway Diagram
APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies	APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway antibodies	APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway Diagram
APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies	APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies	Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
Activation Of NIMA Kinases NEK9, NEK6, NEK7 Pathway antibodies	Activation Of NIMA Kinases NEK9, NEK6, NEK7 Pathway Diagram
C-MYB Transcription Factor Network Pathway antibodies	C-MYB Transcription Factor Network Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Checkpoints Pathway antibodies	Cell Cycle Checkpoints Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram

Diseases associated with anti-CCNB1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>1295 publications with CCNB1 and Neoplasms
Adenocarcinoma Antibodies	>230 publications with CCNB1 and Adenocarcinoma
Breast Neoplasms Antibodies	>210 publications with CCNB1 and Breast Neoplasms
Drug Toxicity Antibodies	>131 publications with CCNB1 and Drug Toxicity
Lung Neoplasms Antibodies	>128 publications with CCNB1 and Lung Neoplasms
Neoplasms, Experimental Antibodies	>113 publications with CCNB1 and Neoplasms, Experimental
Carcinoma, Squamous Cell Antibodies	>112 publications with CCNB1 and Carcinoma, Squamous Cell
Liver Neoplasms Antibodies	>105 publications with CCNB1 and Liver Neoplasms
Liver Diseases Antibodies	>104 publications with CCNB1 and Liver Diseases
Prostatic Neoplasms Antibodies	>92 publications with CCNB1 and Prostatic Neoplasms

Organs/Tissues associated with anti-CCNB1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>201 publications with CCNB1 and Blood
Lung Antibodies	>185 publications with CCNB1 and Lung
Liver Antibodies	>181 publications with CCNB1 and Liver
Prostate Antibodies	>118 publications with CCNB1 and Prostate
Brain Antibodies	>100 publications with CCNB1 and Brain
Vascular Antibodies	>92 publications with CCNB1 and Vascular
Muscle Antibodies	>77 publications with CCNB1 and Muscle
Skin Antibodies	>72 publications with CCNB1 and Skin
Ovary Antibodies	>70 publications with CCNB1 and Ovary
Bone Antibodies	>64 publications with CCNB1 and Bone