• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CD117 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6468217

MBS6468217 is an antibody targeting CD117. Also known as CD117 (c-kit, Stem Cell Factor, SCF Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF) (BSA & Azide Free) (FITC). Reactive in Human. Host: Rabbit. More details

$1020 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD117, Polyclonal Antibody
Full Product Name
CD117 (c-kit, Stem Cell Factor, SCF Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF) (BSA & Azide Free) (FITC)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human CD117 at 145kD.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Molecular Weight
109,865 Da
Chromosome Location
Chromosome: 4; NC_000004.11 (55524095..55606881). Location: 4q11-q12
OMIM
164920
3D Structure
ModBase 3D Structure for P10721
Immunogen
A synthetic peptide from C-terminus of human CD117/c-kit protein
Conjugate
FITC
Positive Control
Tonsil, GI Stromal Tumor (GIST).
Note
Preservative Free

Product Categories/Family for anti-CD117 antibody

Applications Tested/Suitable for anti-CD117 antibody
Immunohistochemistry (IHC) Paraffin
Application Notes for anti-CD117 antibody
IHC-P: 1:400-1:800 for 30 min at RT. Excellent for staining of formalin-fixed, paraffin-embedded tissues.
Applications are based on unconjugated antibody.

Product References and Citations for anti-CD117 antibody

1. Arber D A, et al. Hum. Pathol. 29(5): 498-504 (1998). 2. Rajpert-De Meyts E, et al. Journal of Pathology. 178: 166-169(1996). Additional: 1. Besmer P, Murphy JE, George PC, Qiu F, Bergold PJ, Lederman L, Snyder HW Jr, Brodeur D, Zuckerman EE and Hardy WD (1986) A new acute transforming feline retrovirus and relationship of its oncogene v-kit with the protein kinase family. Nature 320, 415-421. 2. Yarden Y, Kuang W-J, Yang-Feng T, Coussens L, Munemitsu S, Dull TJ, Chen E, Schlessinger J, Francke U and Ullrich A (1987) Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO J 6, 3341-3351. 3. Chabot B, Stephenson DA, Chapman VM, Besmer P and Bernstein A (1988) The proto-oncogene c-kit encoding a transmembrane tyrosine kinase receptor maps to the mouse W locus. Nature 335, 88-89. 4. Geissler EN, Ryan MA and Housman DE (1988) The dominant white spotting (W) locus of the mouse encodes the c-kit proto-oncogene. Cell 55, 185-192. 5. Hoo JJ, Haslam RHA and van Orman (1986) Tentative assignment of piebald trait gene to chromosome band 4q12. Hum Genet 73, 230-231. 6. Qiu F, Ray P, Brown K, Barker PE, Jhanwar S, Ruddle FH and Besmer P (1988) Primary structure of c-kit: relationship with the CSF-1/PDGF receptor kinase family-oncogenic activation of v-kit involves deletion of extracellular domain and C terminus. EMBO J 7, 1003-1011. 7. Russell ES (1949) Analysis of pleiotropism at the W locus in the mouse: Relationship between the effects of W and WV substitution on hair pigmentation and on erythrocytes. Genetics 34, 398-402. 8. Sarvella PA and Russell ES (1956) Steel, a new dominant gene in the house mouse. J Hered 47, 123-128. 9. Ebbe S, Phalen E and Stolman FJ (1973) Abnormal megakaryocytopoiesis in W/WV mice. and: Abnormal megakaryocytopoiesis in Sl/Sld mice. Blood 42, 857-864 and 865-871. 10. Nishimune Y, Haneji T and Kitamura Y (1980) The effects of Steel mutation on testicular germ cell differentiation. J Cell Physiol 105, 137-141. 11. Morrison-Graham K and Weston JA (1989) Mouse mutants provide new insights into the role of extracellular matrix in cell migration and differentiation. Trends Genet 5, 116-121. 12. Harrison DE and Russell ES (1972) The response of W/WV and Sl/Sld anemic mice to haematopoietic stimuli. Br J Haematol 22, 155-168. 13. Kitamura Y, Go S and Hatanaka K (1978) Decrease of mast cells in W/WV mice and their increase by bone marrow transplantation. Blood 52, 447-452. 14. Jarboe DJ and Huff TF (1989) W/WV mice do not make mast cell-committed progenitors and Sl/Sld fibroblasts do not support development of normal mast cell committed progenitors. J Immunol 7, 2418-2423. 15. Williams DE, Eisenman J, Baird A, Rauch C, van Ness K, March CJ, Park LS, Martin U, Mochizuki DY, Boswell HS, Burgess GS, Cosman D and Lyman SD (1990) Identification of a ligand for the c-kit proto-oncogene. Cell 63, 167-174. 16. Zsebo KM, Wypych J, McNiece IK, Lu HS, Smith KA, Karkare S, Sachdev RK; Yuschenkoff VN, Birkett NC, Williams LR, Satyagal VN, Tung W, Bosselman RA, Mendiaz EA and Langley KE (1990) Identification, purification and biological characterization of stem cell factor from Buffalo rat liver conditioned medium. Cell 63, 195-201. 17. Martin FH, Suggs SV, Langley KE, Lu HS, Ting J, Okino KH, Morris FH, McNiece IK, Jacobsen RW, Mendiaz EA, Birkett NC, Smith KA, Johnson MJ, Parker VP, Flores JC; Patel AC, Fisher EF, Erjavec HO, Wypych J, Sachdev RK, Pope JA, Leslie I, Wen D, Lin CH, Cupples RL and Zsebo KM (1990) Primary structure and functional expression of rat and human stem cell factor. Cell 63, 203-211. 18. Zsebo KM, Williams DA, Geissler EN, Broudy VC, Martin FH, Atkins HL, Hsu R-Y, Birkett NC, Okino KH, Murdock DC, Jacobsen RW, Langley KE, Smith KA, Takeishi T, Cattanach BM, Galli SJ and Suggs SV (1990) Stem cell factor is encoded at the Sl locus of the mouse and is the ligand for the c-kit tyrosine kinase receptor. Cell 63, 213-224. 19. Huang E, Nocka K, Beier D, Chu T-Y, Buck J, Lahm H-W, Wellner D, Leder P and Besmer P (1990) The hematopoietic growth factor KL is encoded by the Sl locus and is the ligand of the c-kit receptor, the gene product of the W locus. Cell 63, 225-233. 20. Anderson DM, Lyman SD, Baird A, Wignall JM, Eisenman J, Rauch C, March CJ, Boswell HS, Gimpel SD, Cosman D and Williams DE (1990) Molecular cloning of mast cell growth factor, a hematopoietin that is active in both membrane bound and soluble forms. Cell 63, 235-243. 21. Copeland NG, Gilbert DJ, Cho BC, Donovan PJ, Jenkins NA, Cosman D, Anderson DM, Lyman SD and Williams DE (1990) Mast cell growth factor maps near the Sl locus on mouse chromosome 10 and is deleted in a number of Steel alleles. Cell 63, 175-183. 22. Flanagan JG, Chan DC and Leder P (1991) Transmembrane form of the kit ligand growth factor is determined by alternative splicing and is missing in the Sld mutant. Cell 64, 1025-1035. 23. Toksoz D, Zsebo KM, Smith KA, Hu S, Brankow D, Suggs SV, Martin FH and Wiliams DA (1992) Support of human hematopoiesis in long-term bone marrow culture by murine stromal cells selectively expressing the membrane-bound and secreted forms of the human homolog of the steel gene product, stem cell factor. Proc Natl Acad Sci USA 89, 7350-7354. 24. Nocka K, Majumder S, Chabot B, Ray P, Cervon M, Bernstein A and Besmer P (1989) Expression of c-kit gene products in known cellular targets of W mutations in normal and W mutant mice, evidence for an impaired c-kit kinase in mutant mice. Genes Dev 3, 816-826.

Other Notes

Small volumes of anti-CD117 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD117. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
mast/stem cell growth factor receptor Kit isoform 1
NCBI Official Synonym Full Names
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
NCBI Official Symbol
KIT
NCBI Official Synonym Symbols
PBT; SCFR; C-Kit; CD117
UniProt Protein Name
Mast/stem cell growth factor receptor Kit
UniProt Synonym Protein Names
Piebald trait protein; PBT; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
UniProt Gene Name
KIT
UniProt Synonym Gene Names
SCFR; SCFR; PBT
UniProt Entry Name
KIT_HUMAN
NCBI Protein Information
mast/stem cell growth factor receptor Kit; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like

NCBI Summary for CD117

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for CD117

Kit: a receptor tyrosine kinase and a member of the subfamily that includes PDGF, CSF-1 and FLT-3/flk-2 receptors. Receptor for stem cell factor. Plays a critical role in hematopoietic stem cell, mast cell, melanocyte and germ cell development. Ligand binding induces autophosphorylation, dimerization and activation, leading to the recruitment and phosphorylation of downstream SH2-containing signaling components including PLC-gamma, PI3 kinase p85, SHP2 and CrkL, linking c-Kit to various cell signaling pathways. Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders, while mutations that constitutively activate c-Kit can lead to hyperplasia and tumorigenesis. Activating mutations cause >90% of gastrointestinal stromal tumors (GIST); successfully treated with inhibitors Gleevec (imatinib, Glivec) and Sutent (Sutinib, SU11248). Activating mutations also induce mastocytosis. Autocrine/paracrine stimulation may drive some lung and other tumors. Loss of expression associated with melanoma progression. Familial loss of function mutations cause piebaldism, with defects in hair and skin pigmentation due to lack of melanocytes.

Protein type: Membrane protein, integral; Kinase, protein; Protein kinase, TK; Oncoprotein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; PDGFR family

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: extracellular space; internal side of plasma membrane; mast cell granule; acrosome; plasma membrane; integral to membrane; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; protein homodimerization activity; protease binding; metal ion binding; protein-tyrosine kinase activity; cytokine binding; stem cell factor receptor activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: nerve growth factor receptor signaling pathway; activation of MAPK activity; somatic stem cell maintenance; germ cell programmed cell death; positive regulation of JAK-STAT cascade; lymphoid progenitor cell differentiation; positive regulation of long-term neuronal synaptic plasticity; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of cell shape; epithelial cell proliferation; germ cell migration; erythrocyte differentiation; somatic stem cell division; T cell differentiation; fibroblast growth factor receptor signaling pathway; embryonic hemopoiesis; mast cell chemotaxis; stem cell differentiation; detection of mechanical stimulus involved in sensory perception of sound; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of tyrosine phosphorylation of Stat1 protein; immature B cell differentiation; positive regulation of transcription factor activity; regulation of pigmentation during development; glycosphingolipid metabolic process; mast cell cytokine production; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; signal transduction; myeloid progenitor cell differentiation; ovarian follicle development; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; melanocyte differentiation; negative regulation of programmed cell death; visual learning; hemopoiesis; inflammatory response; positive regulation of Notch signaling pathway; epidermal growth factor receptor signaling pathway; lamellipodium biogenesis; phosphoinositide-mediated signaling; dendritic cell cytokine production; cytokine and chemokine mediated signaling pathway; male gonad development; stem cell maintenance; positive regulation of phosphoinositide 3-kinase activity; mast cell degranulation; regulation of cell proliferation; positive regulation of pseudopodium formation; gut development; positive regulation of tyrosine phosphorylation of Stat5 protein; pigmentation; actin cytoskeleton reorganization; innate immune response; spermatogenesis; spermatid development; positive regulation of cell migration

Disease: Gastrointestinal Stromal Tumor; Mast Cell Disease; Piebald Trait; Testicular Germ Cell Tumor
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000