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CD166 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6282828

CD166 is cell adhesion molecule that binds to CD6. The protein is involved in neurite extension by neurons via heterophilic and homophilic interactions. It may play a role in the binding of T-and B-cells to activated leukocytes, as well as in interactions… More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD166, Polyclonal Antibody
Full Product Name
CD166, NT (ALCAM, MEMD, CD166 antigen, Activated leukocyte cell adhesion molecule, CD166) (AP)
Product Gene Name
Product Synonym Gene Name
ALCAM
Product Synonym Names
ALCAM; MEMD; CD166 antigen; Activated leukocyte cell adhesion molecule; CD166
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
65,102 Da
Chromosome Location
Chromosome: 3; NC_000003.11 (105085557..105295757). Location: 3q13.1
OMIM
601662
3D Structure
ModBase 3D Structure for Q13740
Immunogen
CD166 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65~95 amino acids from the N-terminal region of human ALCAM.
Conjugate
AP
Note
Preservative Free

Product Categories/Family for anti-CD166 antibody

Applications Tested/Suitable for anti-CD166 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-CD166 antibody
FC/FACS: 1:10-50
ELISA: 1:1,000
IHC: 1:50-100
WB: 1:50-100
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-CD166 antibody

Western blot analysis of CD166 antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). CD166 (arrow) was detected using the purified Pab.
Rabbit Anti-CD166 Polyclonal Antibody – Cat# MBS6282828

Immunohistochemistry (IHC) of anti-CD166 antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with CD166 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-CD166 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6282828

Flow Cytometry (FC/FACS) of anti-CD166 antibody

CD166 Antibody (N-term) flow cytometric analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CD166 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6282828

Product References and Citations for anti-CD166 antibody

Kahlert, C., Br. J. Cancer 101 (3), 457-464 (2009). Kulasingam, V., Int. J. Cancer 125 (1), 9-14 (2009).

Other Notes

Small volumes of anti-CD166 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD166 purchase

MBS6282828 is an antibody targeting CD166. Also known as CD166, NT (ALCAM, MEMD, CD166 antigen, Activated leukocyte cell adhesion molecule, CD166) (AP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CD166. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
alcam
NCBI Official Synonym Full Names
activated leukocyte cell adhesion molecule
NCBI Official Symbol
ALCAM
NCBI Official Synonym Symbols
MEMD; CD166
UniProt Protein Name
CD166 antigen
UniProt Synonym Protein Names
Activated leukocyte cell adhesion molecule
Protein Family
UniProt Gene Name
ALCAM
UniProt Synonym Gene Names
MEMD
UniProt Entry Name
CD166_HUMAN
NCBI Protein Information
CD166 antigen

NCBI Summary for CD166

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]
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UniProt Comments for CD166

ALCAM: Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 3q13.1

Cellular Component: focal adhesion; cell soma; axon; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: axon guidance; motor axon guidance; signal transduction; cell adhesion
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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