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CD20 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS462026

CD20, a non-glycosylated protein, belongs to a member of the membrane-spanning 4A gene family. The molecular weight is at a range of ~34-37kDa depending on the degree of the protein phosphorylation. The CD20 antigen is present on human pre B lymphocytes and B… More details

$355 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD20, Polyclonal Antibody
Full Product Name
Rabbit anti CD20 antibody
Product Gene Name
Product Synonym Names
Rabbit anti-CD20; MS4A1; CD20; CD20 antigen; CD20 receptor; B-Lymphocyte cell-surface antigen B1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
Purity/Purification
The Rabbit IgG is purified by Epitope Affinity Purification.
Specificity
This antibody recognizes ~34 kDa of human CD20. The other species are not tested
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C - 8 degree C for three months without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Molecular Weight
34.0
3D Structure
ModBase 3D Structure for P11836
Antigen Preparation
A synthetic peptide corresponding to C-terminus of human CD20 protein.
Positive Control
Kidney Tissue
Cellular Location
Cell Membrane
Applications Tested/Suitable for anti-CD20 antibody
ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry-paraffin (IHC-p)
Application Notes for anti-CD20 antibody
WB: 0.1-1 ug/ml

EIA: 0.01-0.1 ug/ml
IP: 2-5 ug/ml
IHC: 2-10 ug/ml
Optimal dilutions should be determined by researchers for the specific applications.

Western Blot (WB) of anti-CD20 antibody

Western Blot: The whole lysates derived from human spleen were immunoblotted by Rabbit anti-CD20 (Cat# MBS462026) at 1:1000. Observed a major immunoreactive band at molecular weight ~34 kDa.
Rabbit Anti-CD20 Polyclonal Antibody – Cat# MBS462026

Immunohistochemistry (IHC) of anti-CD20 antibody

Immunohistochemistry: Human tonsil tissue (FFPE) was stained with Anti-CD20 antibody, (Cat# MBS462026) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Rabbit Anti-CD20 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS462026

Product References and Citations for anti-CD20 antibody

Smith MR, Rituximab (monoclonal anti-CD20 antibody): mechanisms of action and resistance. Oncogene, 2003 Oct 20;22 (47):7359-68.

Other Notes

Small volumes of anti-CD20 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD20 purchase

MBS462026 is an antibody targeting CD20. Also known as Rabbit anti CD20 antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CD20. It may not necessarily be applicable to this product.

UniProt Primary Accession #
Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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