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CD274 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6468292

MBS6468292 is an antibody targeting CD274. Also known as CD274 (B7-h1 Protein, B7-H1, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1, PDCD1LG1, PDL1) (APC). Reactive in Human. Host: Rabbit. More details

$1095 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD274, Polyclonal Antibody
Full Product Name
CD274 (B7-h1 Protein, B7-H1, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1, PDCD1LG1, PDL1) (APC)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by affinity chromatography.
Specificity
Recognizes human CD274. Species Crossreactivity: mouse and rat.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Synthetic peptide corresponding to a 17aa sequence from near the center of human CD274.
Conjugate
APC
Note
Preservative Free

Product Categories/Family for anti-CD274 antibody

Applications Tested/Suitable for anti-CD274 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-CD274 antibody
IHC-P: 2.5ug/ml. Requires antigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate pH 6.0 is recommended.
WB: 0.5-1ug/ml. Detects a band of ~32kD in Raji cell lysate.
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-CD274 antibody

Western Blot analysis of whole cell lysate from Raji, human Burkitt's lymphoma derived cells using C2549-22C at 0.5ug/ml (A) and 1ug/ml (B).
Rabbit Anti-CD274 Polyclonal Antibody – Cat# MBS6468292

Immunohistochemistry (IHC) of anti-CD274 antibody

Immunohistochemical staining of human heart tissue using C2549-22C.
Rabbit Anti-CD274 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6468292

Product References and Citations for anti-CD274 antibody

1. Adriana J., et al. (2006) Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 67:568-78 2. Holling TM, et al. (2004) Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 65:282-90 3. Ishida Y, et al. (1992) Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 11:3887-95.

Other Notes

Small volumes of anti-CD274 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD274. It may not necessarily be applicable to this product.

Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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