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CD50 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9703638

Intercellular adhesion molecule 3 encoded by ICAM3 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CD50, Polyclonal Antibody
Full Product Name
CD50 Polyclonal Antibody
Product Gene Name
Product Synonym Names
Rabbit Anti-CD50 Polyclonal Antibody; ICAM3; Intercellular adhesion molecule 3; ICAM-3; CDw50; ICAM-R; CD50
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid; PBS Containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml (lot specific)
Specificity
CD50 Polyclonal Antibody detects endogenous levels of CD50 protein.
Preparation and Storage
Stable for one year at -20 degree C from date of shipment.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
OMIM
146631
3D Structure
ModBase 3D Structure for P32942
Immunogen
Synthesized peptide derived from the internal region of human CD50
Applications Tested/Suitable for anti-CD50 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
Application Notes for anti-CD50 antibody
WB: 1:500-1:2000
IHC-P: 1:100-1:300
ELISA: 1:20000

Western Blot (WB) of anti-CD50 antibody

Fig.1. Western Blot analysis of K562 cells using CD50 Polyclonal Antibody. Secondary antibody was diluted at 1:20000.
Rabbit Anti-CD50 Polyclonal Antibody – Cat# MBS9703638

Immunohistochemistry (IHC) of anti-CD50 antibody

Fig.2. Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100.
Rabbit Anti-CD50 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9703638

Immunohistochemistry (IHC) of anti-CD50 antibody

Fig.3. Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100.
Rabbit Anti-CD50 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9703638

Other Notes

Small volumes of anti-CD50 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD50 purchase

MBS9703638 is an antibody targeting CD50. Also known as CD50 Polyclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CD50. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
intercellular adhesion molecule 3 isoform 1
NCBI Official Synonym Full Names
intercellular adhesion molecule 3
NCBI Official Symbol
ICAM3
NCBI Official Synonym Symbols
CD50; CDW50; ICAM-R
UniProt Protein Name
Intercellular adhesion molecule 3
UniProt Synonym Protein Names
CDw50; ICAM-R
UniProt Gene Name
ICAM3
UniProt Synonym Gene Names
ICAM-3
UniProt Entry Name
ICAM3_HUMAN
NCBI Protein Information
intercellular adhesion molecule 3

NCBI Summary for CD50

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016]
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UniProt Comments for CD50

ICAM3: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. Belongs to the immunoglobulin superfamily. ICAM family.

Protein type: Cell adhesion; Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: integrin binding; protein binding; receptor binding

Biological Process: cell-cell adhesion; extracellular matrix organization and biogenesis; regulation of immune response; cell adhesion
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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