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CD62L Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6468125

MBS6468125 is an antibody targeting CD62L. Also known as CD62L, CT (L-selectin, Lymph Node Homing Receptor, Leukocyte Adhesion Molecule 1, LAM-1, Leukocyte Surface Antigen Leu-8, TQ1, gp90-MEL, Leukocyte-endothelial Cell Adhesion Molecule 1, LECAM1, CD62… More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD62L, Polyclonal Antibody
Full Product Name
CD62L, CT (L-selectin, Lymph Node Homing Receptor, Leukocyte Adhesion Molecule 1, LAM-1, Leukocyte Surface Antigen Leu-8, TQ1, gp90-MEL, Leukocyte-endothelial Cell Adhesion Molecule 1, LECAM1, CD62 Antigen-like Family Member L, SELL, LYAM1, LNHR) (PE)
Product Gene Name
anti-CD62L antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Immunoaffinity chromatography.
Specificity
Recognizes human SELL.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
42.187
OMIM
153240
3D Structure
ModBase 3D Structure for P14151
Immunogen
Synthetic peptide selected from the C-terminal region of human SELL (KLH).
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-CD62L antibody

Antibodies; CD Markers
Applications Tested/Suitable for anti-CD62L antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-CD62L antibody
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-CD62L antibody

Western blot analysis of SELL Antibody (C-term) in Jurkat cell line lysates (35ug/lane).SELL (arrow) was detected using the purified Pab.
Rabbit Anti-CD62L Polyclonal Antibody – Cat# MBS6468125

Immunohistochemistry (IHC) of anti-CD62L antibody

Formalin-fixed and paraffin-embedded human lymph tissue with SELL Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-CD62L Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6468125

Flow Cytometry (FC/FACS) of anti-CD62L antibody

Flow cytometric analysis of jurkat cells using SELL Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CD62L Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6468125

Product References and Citations for anti-CD62L antibody

1. Zebrowska, A., et.al., Pol J Pathol 60(1), 26-34 (2009).

Other Notes

Small volumes of anti-CD62L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD62L. It may not necessarily be applicable to this product.

NCBI GI #
4506875
NCBI Accession #
NP_000646.1
UniProt Primary Accession #
P14151
GenBank Nucleotide #
NP_000646.1
NCBI Official Full Name
selectin L
UniProt Protein Name
L-selectin
UniProt Synonym Protein Names
CD62 antigen-like family member L; Leukocyte adhesion molecule 1; LAM-1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LECAM1; Lymph node homing receptor; TQ1; gp90-MEL
Protein Family
L-selectin
UniProt Gene Name
SELL
UniProt Synonym Gene Names
LNHR; LYAM1; LAM-1; LECAM1
UniProt Entry Name
LYAM1_HUMAN

UniProt Comments for CD62L

SELL: Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. Belongs to the selectin/LECAM family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23-q25

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: heparin binding; protein binding; protease binding; cell adhesion molecule binding; carbohydrate binding; glycosphingolipid binding

Biological Process: regulation of immune response; cell adhesion; blood coagulation; leukocyte migration; response to ATP

Disease: Iga Nephropathy, Susceptibility To, 1
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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