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CDKN2B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6284712

This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the… More details

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Product Overview

Online Datasheet in PDF
Product Name
CDKN2B, Polyclonal Antibody
Full Product Name
CDKN2B, NT (CDKN2B, MTS2, Cyclin-dependent kinase 4 inhibitor B, Multiple tumor suppressor 2, p14-INK4b, p15-INK4b) (Biotin)
Product Gene Name
anti-CDKN2B antibody
Product Synonym Names
CDKN2B; MTS2; Cyclin-dependent kinase 4 inhibitor B; Multiple tumor suppressor 2; p14-INK4b; p15-INK4b
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
14,722 Da
Chromosome Location
Chromosome: 9; NC_000009.11 (22002902..22009312, complement). Location: 9p21
OMIM
600431
3D Structure
ModBase 3D Structure for P42772
Immunogen
CDKN2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CDKN2B.
Conjugate
Biotin
Note
Preservative Free

Product Categories/Family for anti-CDKN2B antibody

Antibodies; Protein Kinase Inhibitors
Applications Tested/Suitable for anti-CDKN2B antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-CDKN2B antibody
FC/FACS: 1:10-50
ELISA: 1:1,000
IF: 1:10-50
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-CDKN2B antibody

Western blot analysis of CDKN2B (arrow) using rabbit polyclonal CDKN2B Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDKN2B gene.
Rabbit Anti-CDKN2B Polyclonal Antibody – Cat# MBS6284712

Immunohistochemistry (IHC) of anti-CDKN2B antibody

CDKN2B Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CDKN2B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-CDKN2B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6284712

Immunofluorescence (IF) of anti-CDKN2B antibody

Confocal immunofluorescent analysis of CDKN2B Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-CDKN2B Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6284712

Flow Cytometry (FC/FACS) of anti-CDKN2B antibody

CDKN2B Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CDKN2B Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6284712

Product References and Citations for anti-CDKN2B antibody

Camacho, C.V., et al. Carcinogenesis 31(10):1889-1896(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Pechlivanis, S., et al. Arterioscler. Thromb. Vasc. Biol. 30(9):1867-1872(2010). Heni, M., et al. Diabetes (2010). In press: Roder, C., et al. Childs Nerv Syst (2010).

Other Notes

Small volumes of anti-CDKN2B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDKN2B purchase

MBS6284712 is an antibody targeting CDKN2B. Also known as CDKN2B, NT (CDKN2B, MTS2, Cyclin-dependent kinase 4 inhibitor B, Multiple tumor suppressor 2, p14-INK4b, p15-INK4b) (Biotin). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CDKN2B. It may not necessarily be applicable to this product.

NCBI GI #
17981694
NCBI GeneID
1030
NCBI Accession #
NP_004927.2
UniProt Primary Accession #
P42772
UniProt Secondary Accession #
O15125; Q6FI09
UniProt Related Accession #
P42772; AAB32712
GenBank Nucleotide #
NM_004936.3
NCBI Official Full Name
cyclin-dependent kinase 4 inhibitor B isoform 1
NCBI Official Synonym Full Names
cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)
NCBI Official Symbol
CDKN2B
NCBI Official Synonym Symbols
P15; MTS2; TP15; CDK4I; INK4B; p15INK4b
UniProt Protein Name
Cyclin-dependent kinase 4 inhibitor B
UniProt Synonym Protein Names
Multiple tumor suppressor 2; MTS-2; p14-INK4b; p15-INK4b
Protein Family
Cyclin-dependent kinase 4 inhibitor
UniProt Gene Name
CDKN2B
UniProt Synonym Gene Names
MTS2; MTS-2; p15INK4B
UniProt Entry Name
CDN2B_HUMAN
NCBI Protein Information
cyclin-dependent kinase 4 inhibitor B; MTS-2; p14-INK4b; p14_INK4B; p15-INK4b; p15_INK4B; CDK4B inhibitor; p14_CDK inhibitor; p15 CDK inhibitor; CDK inhibitory protein; multiple tumor suppressor 2; cyclin-dependent kinases 4 and 6 binding protein

UniProt Comments for CDKN2B

p15-INK4B: a potent inhibitor that nteracts strongly with CDK4 and CDK6. Potential effector of TGF-beta induced cell cycle arrest.

Protein type: Cell cycle regulation; Inhibitor; Tumor suppressor; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; protein kinase binding

Biological Process: spleen development; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; megakaryocyte differentiation; mitotic cell cycle checkpoint; positive regulation of transforming growth factor beta receptor signaling pathway; cellular response to nutrient; liver development; response to organic cyclic substance; negative regulation of cell proliferation; transforming growth factor beta receptor signaling pathway; response to cytokine stimulus; cellular response to extracellular stimulus; positive regulation of epithelial cell differentiation; negative regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; gene expression; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; cell cycle arrest; G2/M transition of mitotic cell cycle; negative regulation of epithelial cell proliferation; aging
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Research Articles on CDKN2B

1. Single nucleotide polymorphisms in CDKN2B gene is associated with neoplasms

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CDKN2B antibody

 Reference Product  PubMed Publications
 CDK4 antibody  >117 publications with CDKN2B and CDK4
 ZBTB17 antibody  >7 publications with CDKN2B and ZBTB17

Pathways associated with anti-CDKN2B antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cyclin D Associated Events In G1 Pathway antibodies  Cyclin D Associated Events In G1 Pathway Diagram
 G1 Phase Pathway antibodies  G1 Phase Pathway Diagram
 G1 To S Cell Cycle Control Pathway antibodies  G1 To S Cell Cycle Control Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram

Diseases associated with anti-CDKN2B antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >622 publications with CDKN2B and Neoplasms
 Leukemia Antibodies  >205 publications with CDKN2B and Leukemia
 Immune System Diseases Antibodies  >160 publications with CDKN2B and Immune System Diseases
 Carcinoma Antibodies  >151 publications with CDKN2B and Carcinoma
 Lymphoma Antibodies  >149 publications with CDKN2B and Lymphoma
 Chromosome Aberrations Antibodies  >101 publications with CDKN2B and Chromosome Aberrations
 Leukemia, Myeloid, Acute Antibodies  >88 publications with CDKN2B and Leukemia, Myeloid, Acute
 Glioma Antibodies  >58 publications with CDKN2B and Glioma
 Carcinoma, Squamous Cell Antibodies  >48 publications with CDKN2B and Carcinoma, Squamous Cell
 Myelodysplastic Syndromes Antibodies  >44 publications with CDKN2B and Myelodysplastic Syndromes

Organs/Tissues associated with anti-CDKN2B antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >62 publications with CDKN2B and Brain
 Lung Antibodies  >50 publications with CDKN2B and Lung
 Liver Antibodies  >44 publications with CDKN2B and Liver
 Skin Antibodies  >34 publications with CDKN2B and Skin
 Muscle Antibodies  >22 publications with CDKN2B and Muscle
 Prostate Antibodies  >18 publications with CDKN2B and Prostate
 Bladder Antibodies  >18 publications with CDKN2B and Bladder
 Stomach Antibodies  >15 publications with CDKN2B and Stomach
 Embryonic Tissue Antibodies  >10 publications with CDKN2B and Embryonic Tissue
 Kidney Antibodies  >9 publications with CDKN2B and Kidney
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