• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CEBPB Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6468548

MBS6468548 is an antibody targeting CEBPB. Also known as CEBPB, CT (CCAAT/Enhancer-binding Protein beta, C/EBP beta, Liver Activator Protein, Nuclear factor NF-IL6, Transcription factor 5, PP9092, TCF5, LAP) (HRP). Reactive in Human. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CEBPB, Polyclonal Antibody
Full Product Name
CEBPB, CT (CCAAT/Enhancer-binding Protein beta, C/EBP beta, Liver Activator Protein, Nuclear factor NF-IL6, Transcription factor 5, PP9092, TCF5, LAP) (HRP)
Product Gene Name
anti-CEBPB antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human CEBPB.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Molecular Weight
15,938 Da
OMIM
189965
3D Structure
ModBase 3D Structure for P17676
Immunogen
Synthetic peptide selected from the C-terminal region of human CEBPB (KLH).
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-CEBPB antibody

Antibodies; Transcription Factors
Applications Tested/Suitable for anti-CEBPB antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-CEBPB antibody
WB: 1:50-1:100
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-CEBPB antibody

Western blot analysis of CEBPB (arrow) using rabbit polyclonal CEBPB Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CEBPB gene (Lane 2).
Rabbit Anti-CEBPB Polyclonal Antibody – Cat# MBS6468548

Immunohistochemistry (IHC) of anti-CEBPB antibody

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CEBPB Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-CEBPB Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6468548

Flow Cytometry (FC/FACS) of anti-CEBPB antibody

CEBPB Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CEBPB Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6468548

Product References and Citations for anti-CEBPB antibody

1. Buck, M., et.al., Mol. Cell 4(6), 1087-1092 (1999).

Other Notes

Small volumes of anti-CEBPB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CEBPB. It may not necessarily be applicable to this product.

NCBI GI #
28872796
NCBI GeneID
1051
NCBI Accession #
NP_005185
UniProt Primary Accession #
P17676
UniProt Secondary Accession #
Q96IH2; Q9H4Z5; A8K671
UniProt Related Accession #
P17676
GenBank Nucleotide #
NM_005194.3
NCBI Official Full Name
CCAAT/enhancer-binding protein beta isoform a
NCBI Official Synonym Full Names
CCAAT/enhancer binding protein beta
NCBI Official Symbol
CEBPB
NCBI Official Synonym Symbols
TCF5; IL6DBP; NF-IL6; C/EBP-beta
UniProt Protein Name
CCAAT/enhancer-binding protein beta
UniProt Synonym Protein Names
Liver activator protein; Nuclear factor NF-IL6; Transcription factor 5
Protein Family
CCAAT/enhancer-binding protein
UniProt Gene Name
CEBPB
UniProt Synonym Gene Names
LAP; TCF5; C/EBP beta; TCF-5
UniProt Entry Name
CEBPB_HUMAN
NCBI Protein Information
CCAAT/enhancer-binding protein beta

NCBI Summary for CEBPB

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. [provided by RefSeq, Oct 2013]
Show more

UniProt Comments for CEBPB

C/EBP-beta: a bZIP transcription factor which can form homodimers or heterodimers with the related proteins CEBP-alpha, -delta and -gamma. Involved in immune and inflammatory responses. Specifically binds to regulatory regions of genes encoding IL-6, other cytokines and several acute-phase proteins. There are two forms of C/EBPbeta, the 38kDa liver activating protein (LAP) and the 20kDa liver inhibitory protein (LIP) which may be products of alternative translation. LAP is a transcriptional activator while LIP may inhibit C/EBPbeta transcriptional activity. Phosphorylated and activated by ERK1/2.

Protein type: Motility/polarity/chemotaxis; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 20q13.1

Cellular Component: nucleoplasm; nuclear matrix; nuclear chromatin; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; chromatin binding; transcription factor activity; transcription factor binding; glucocorticoid receptor binding

Biological Process: transcription from RNA polymerase II promoter; embryonic placenta development; regulation of interleukin-6 biosynthetic process; mammary gland epithelial cell proliferation; response to lipopolysaccharide; neuron differentiation; positive regulation of osteoblast differentiation; regulation of transcription, DNA-dependent; brown fat cell differentiation; acute-phase response; positive regulation of fat cell differentiation; immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of neuron apoptosis; inflammatory response; negative regulation of transcription, DNA-dependent
Show more

Research Articles on CEBPB

1. C/EBPbeta overexpression or knockdown did not change the levels of IL-1beta-induced SOCS1. SOCS1 regulated the levels of C/EBPbeta mRNA by ubiquitination of C/EBPbeta as well as transcriptional regulation in human chondrocytes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CEBPB antibody

 Reference Product  PubMed Publications
 CEBPA antibody  >38 publications with CEBPB and CEBPA
 CEBPD antibody  >20 publications with CEBPB and CEBPD
 DDIT3 antibody  >7 publications with CEBPB and DDIT3
 MYC antibody  >6 publications with CEBPB and MYC
 SP1 antibody  >5 publications with CEBPB and SP1
 NFKB1 antibody  >5 publications with CEBPB and NFKB1
 CREB1 antibody  >4 publications with CEBPB and CREB1
 RUNX2 antibody  >4 publications with CEBPB and RUNX2
 EGR1 antibody  >4 publications with CEBPB and EGR1
 CEBPG antibody  >3 publications with CEBPB and CEBPG

Pathways associated with anti-CEBPB antibody

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Differentiation Of White And Brown Adipocyte Pathway antibodies  Differentiation Of White And Brown Adipocyte Pathway Diagram
 Diurnally Regulated Genes With Circadian Orthologs Pathway antibodies  Diurnally Regulated Genes With Circadian Orthologs Pathway Diagram
 FOXA1 Transcription Factor Network Pathway antibodies  FOXA1 Transcription Factor Network Pathway Diagram
 FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies  FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
 Folate-Alcohol And Cancer Pathway antibodies  Folate-Alcohol And Cancer Pathway Diagram

Diseases associated with anti-CEBPB antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >39 publications with CEBPB and Neoplasms
 Inflammation Antibodies  >18 publications with CEBPB and Inflammation
 Necrosis Antibodies  >8 publications with CEBPB and Necrosis
 Brain Diseases Antibodies  >7 publications with CEBPB and Brain Diseases
 Nervous System Diseases Antibodies  >7 publications with CEBPB and Nervous System Diseases
 Lung Diseases Antibodies  >6 publications with CEBPB and Lung Diseases
 Fatty Liver Antibodies  >5 publications with CEBPB and Fatty Liver
 Cardiovascular Diseases Antibodies  >4 publications with CEBPB and Cardiovascular Diseases
 Liver Diseases Antibodies  >4 publications with CEBPB and Liver Diseases
 Cell Transformation, Neoplastic Antibodies  >4 publications with CEBPB and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-CEBPB antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >28 publications with CEBPB and Blood
 Bone Antibodies  >22 publications with CEBPB and Bone
 Liver Antibodies  >21 publications with CEBPB and Liver
 Bone Marrow Antibodies  >16 publications with CEBPB and Bone Marrow
 Muscle Antibodies  >11 publications with CEBPB and Muscle
 Lung Antibodies  >11 publications with CEBPB and Lung
 Brain Antibodies  >10 publications with CEBPB and Brain
 Embryonic Tissue Antibodies  >5 publications with CEBPB and Embryonic Tissue
 Heart Antibodies  >5 publications with CEBPB and Heart
 Uterus Antibodies  >5 publications with CEBPB and Uterus
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000