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CENPH Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6285097

Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CENPH, Polyclonal Antibody
Full Product Name
CENPH, ID (CENPH, ICEN35, Centromere protein H, Interphase centromere complex protein 35) (Biotin)
Product Gene Name
anti-CENPH antibody
Product Synonym Names
CENPH; ICEN35; Centromere protein H; Interphase centromere complex protein 35
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
28,481 Da
Chromosome Location
Chromosome: 5; NC_000005.9 (68485375..68506184). Location: 5p15.2
OMIM
605607
3D Structure
ModBase 3D Structure for Q9H3R5
Immunogen
CENPH antibody is generated from rabbits immunized with a KLH conjugated Synthetic peptide selected from the center region of human CENPH.
Conjugate
Biotin
Note
Preservative Free

Product Categories/Family for anti-CENPH antibody

Antibodies; Centromeres
Applications Tested/Suitable for anti-CENPH antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-CENPH antibody
FC/FACS: 1:10-50
ELISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-CENPH antibody

Western blot analysis of CENPH Antibody (Center) in Hela cell line lysates (35ug/lane). CENPH (arrow) was detected using the purified Pab.
Rabbit Anti-CENPH Polyclonal Antibody – Cat# MBS6285097

Immunohistochemistry (IHC) of anti-CENPH antibody

Formalin-fixed and paraffin-embedded human testis tissue reacted with CENPH Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-CENPH Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6285097

Flow Cytometry (FC/FACS) of anti-CENPH antibody

CENPH Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CENPH Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6285097

Other Notes

Small volumes of anti-CENPH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CENPH purchase

MBS6285097 is an antibody targeting CENPH. Also known as CENPH, ID (CENPH, ICEN35, Centromere protein H, Interphase centromere complex protein 35) (Biotin). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CENPH. It may not necessarily be applicable to this product.

NCBI GI #
12597655
NCBI GeneID
64946
NCBI Accession #
NP_075060.1
UniProt Primary Accession #
Q9H3R5
UniProt Secondary Accession #
A8K3Y1
UniProt Related Accession #
Q9H3R5
GenBank Nucleotide #
NM_022909.3
NCBI Official Full Name
centromere protein H
NCBI Official Synonym Full Names
centromere protein H
NCBI Official Symbol
CENPH
UniProt Protein Name
Centromere protein H
UniProt Synonym Protein Names
Interphase centromere complex protein 35
Protein Family
Centromere protein
UniProt Gene Name
CENPH
UniProt Synonym Gene Names
ICEN35; CENP-H
UniProt Entry Name
CENPH_HUMAN
NCBI Protein Information
centromere protein H; CENP-H; kinetochore protein CENP-H; interphase centromere complex protein 35

UniProt Comments for CENPH

CENPH: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate. Belongs to the centromere protein H family.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: nucleoplasm; kinetochore; nucleus; cytosol

Molecular Function: kinetochore binding; protein binding

Biological Process: nucleosome assembly; kinetochore assembly; DNA replication-independent nucleosome assembly at centromere; mitotic cell cycle; kinetochore organization and biogenesis
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Research Articles on CENPH

1. High expression of CENP-H in gastric cancer indicates poor prognosis and Survivin may mediate its procancer role.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CENPH antibody

 Reference Product  PubMed Publications
 NDC80 antibody  >6 publications with CENPH and NDC80
 CENPA antibody  >1 publications with CENPH and CENPA
 BIRC5 antibody  >1 publications with CENPH and BIRC5
 CENPK antibody  >1 publications with CENPH and CENPK
 NUF2 antibody  >1 publications with CENPH and NUF2

Pathways associated with anti-CENPH antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway antibodies  Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Mitotic Anaphase Pathway antibodies  Mitotic Anaphase Pathway Diagram
 Mitotic Metaphase And Anaphase Pathway antibodies  Mitotic Metaphase And Anaphase Pathway Diagram
 Mitotic Prometaphase Pathway antibodies  Mitotic Prometaphase Pathway Diagram
 Nucleosome Assembly Pathway antibodies  Nucleosome Assembly Pathway Diagram
 Resolution Of Sister Chromatid Cohesion Pathway antibodies  Resolution Of Sister Chromatid Cohesion Pathway Diagram

Diseases associated with anti-CENPH antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >14 publications with CENPH and Neoplasms
 Neoplasm Metastasis Antibodies  >2 publications with CENPH and Neoplasm Metastasis
 Carcinoma, Squamous Cell Antibodies  >2 publications with CENPH and Carcinoma, Squamous Cell
 Liver Diseases Antibodies  >2 publications with CENPH and Liver Diseases
 Immune System Diseases Antibodies  >1 publications with CENPH and Immune System Diseases
 Lung Neoplasms Antibodies  >1 publications with CENPH and Lung Neoplasms
 Liver Neoplasms Antibodies  >1 publications with CENPH and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >1 publications with CENPH and Carcinoma, Hepatocellular
 Liver Cirrhosis Antibodies  >1 publications with CENPH and Liver Cirrhosis
 Neoplasm Invasiveness Antibodies  >1 publications with CENPH and Neoplasm Invasiveness

Organs/Tissues associated with anti-CENPH antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3 publications with CENPH and Blood
 Embryonic Tissue Antibodies  >2 publications with CENPH and Embryonic Tissue
 Bone Antibodies  >1 publications with CENPH and Bone
 Lymph Node Antibodies  >1 publications with CENPH and Lymph Node
 Lymph Antibodies  >1 publications with CENPH and Lymph
 Bone Marrow Antibodies  >1 publications with CENPH and Bone Marrow
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