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CERS4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9216892

MBS9216892 is an antibody targeting CERS4. Also known as CERS4 Antibody (N-Term). Reactive in Human. Host: Rabbit. More details

$240 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CERS4, Polyclonal Antibody
Full Product Name
CERS4 Antibody (N-Term)
Product Gene Name
anti-CERS4 antibody
Product Synonym Names
Purified Rabbit Polyclonal Antibody (Pab); Ceramide synthase 4; CerS4; LAG1 longevity assurance homolog 4; CERS4; LASS4
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Specificity
This CERS4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 21-51 amino acids from human CERS4.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
CERS4 peptide (MBS9230571) is used for blocking the activity of CERS4 antibody (MBS9216892)
Molecular Weight
46399
OMIM
615334
3D Structure
ModBase 3D Structure for Q9HA82
Function
Ceramide synthase that catalyzes formation of ceramide from sphinganine and acyl-CoA substrates, with high selectivity toward long and very-long chains (C18:0-C22:0) as acyl donor.
Cellular Location
Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9D6J1}; Multi-pass membrane protein

Product Categories/Family for anti-CERS4 antibody

Crown Antibodies; Cancer; Metabolism; Signal Transduction
Applications Tested/Suitable for anti-CERS4 antibody
Western Blot (WB)
Application Notes for anti-CERS4 antibody
WB~~1:2000
FC~~1:25

Western Blot (WB) of anti-CERS4 antibody

All lanes : Anti-CERS4 Antibody (N-Term) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HT-1080 whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: LNCaP whole cell lysate
Lane 5: PC-3 whole cell lysate
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 46 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-CERS4 Polyclonal Antibody – Cat# MBS9216892

Flow Cytometry (FC) of anti-CERS4 antibody

Overlay histogram showing Hela cells stained with MBS9216892 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9216892, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Rabbit Anti-CERS4 Polyclonal Antibody, Flow Cytometry (FC) – Cat# MBS9216892

Product References and Citations for anti-CERS4 antibody

Ota T.,et al.Nat. Genet. 36:40-45(2004). Grimwood J.,et al.Nature 428:529-535(2004). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

Other Notes

Small volumes of anti-CERS4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CERS4. It may not necessarily be applicable to this product.

NCBI GI #
205360977
NCBI GeneID
79603
NCBI Accession #
NP_078828.2
UniProt Primary Accession #
Q9HA82
UniProt Secondary Accession #
D6W665
UniProt Related Accession #
Q9HA82
GenBank Nucleotide #
NM_024552.2
NCBI Official Full Name
ceramide synthase 4
NCBI Official Synonym Full Names
ceramide synthase 4
NCBI Official Symbol
CERS4
NCBI Official Synonym Symbols
Trh1; LASS4
UniProt Protein Name
Ceramide synthase 4
UniProt Synonym Protein Names
LAG1 longevity assurance homolog 4
Protein Family
Ceramide synthase
UniProt Gene Name
CERS4
UniProt Synonym Gene Names
LASS4; CerS4
UniProt Entry Name
CERS4_HUMAN
NCBI Protein Information
ceramide synthase 4

UniProt Comments for CERS4

Lass4: May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing different fatty acid donors (N-linked stearoyl- (C18) or arachidoyl- (C20) ceramides) in a fumonisin B1-independent manner.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; DNA-binding

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: endoplasmic reticulum membrane; integral to membrane; nuclear membrane

Molecular Function: DNA binding; sphingosine N-acyltransferase activity

Biological Process: ceramide biosynthetic process; sphingolipid biosynthetic process; sphingolipid metabolic process
Show more

Research Articles on CERS4

1. mRNA level of CerS4 is higher in cancerous cell lines and decreases following the induction of apoptosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CERS4 antibody

 Reference Product  PubMed Publications
 CERK antibody  >1 publications with CERS4 and CERK
 SPHK2 antibody  >1 publications with CERS4 and SPHK2
 SPHK1 antibody  >1 publications with CERS4 and SPHK1

Pathways associated with anti-CERS4 antibody

 Products by Pathway  Pathway Diagram
 Ceramide Biosynthesis Pathway antibodies  Ceramide Biosynthesis Pathway Diagram
 Ceramide Biosynthesis Pathway antibodies  Ceramide Biosynthesis Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid De Novo Biosynthesis Pathway antibodies  Sphingolipid De Novo Biosynthesis Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram

Diseases associated with anti-CERS4 antibody

 Disease Name  Pubmed Publications
 Edema Antibodies  >1 publications with CERS4 and Edema

Organs/Tissues associated with anti-CERS4 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >2 publications with CERS4 and Lung
 Liver Antibodies  >2 publications with CERS4 and Liver
 Skin Antibodies  >2 publications with CERS4 and Skin
 Brain Antibodies  >2 publications with CERS4 and Brain
 Bone Antibodies  >1 publications with CERS4 and Bone
 Nerve Antibodies  >1 publications with CERS4 and Nerve
 Muscle Antibodies  >1 publications with CERS4 and Muscle
 Pancreas Antibodies  >1 publications with CERS4 and Pancreas
 Kidney Antibodies  >1 publications with CERS4 and Kidney
 Blood Antibodies  >1 publications with CERS4 and Blood
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