Rabbit Anti-CHCHD4 Polyclonal Antibody – Cat# MBS3905908

Detection of human CHCHD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CHCHD4 antibody (MBS3905908 lot 1) used for IP at 6 ug per reaction. CHCHD4 was also immunoprecipitated by rabbit anti-CHCHD4 antibody MBS3904976. For blotting immunoprecipitated CHCHD4, MBS3905909 was used at 0.4 mg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

CHCHD4 Antibody – Rabbit Polyclonal

Name: CHCHD4 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS3905908
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3905908

CHCHD4 functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17, COX19 and MICU1. Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space… More details

$250 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CHCHD4, Polyclonal Antibody

Full Product Name

Rabbit anti-CHCHD4 Antibody, Affinity Purified

Product Gene Name

anti-CHCHD4 antibody

Product Synonym Names

translocase of inner mitochondrial membrane 40 homolog; TIMM40; coiled-coil-helix-coiled-coil-helix domain-containing protein 4; mitochondrial intermembrane space import and assembly protein 40; MIA40; Mitochondrial intermembrane space import and assembly protein 40; coiled-coil-helix-coiled-coil-helix domain containing 4

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Purity/Purification

Antigen Affinity Purified

Concentration

1000ug/ml (lot specific)

Preparation and Storage

Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

OMIM

611077

3D Structure

ModBase 3D Structure for Q8N4Q1

Immunogen

Between 92 and C-terminus

Conjugation

Unconjugated

Product Categories/Family for anti-CHCHD4 antibody

Primary Antibodies

Applications Tested/Suitable for anti-CHCHD4 antibody

Immunoprecipitation (IP)

Application Notes for anti-CHCHD4 antibody

IP: 6ug/mg lysate

Other Notes

Small volumes of anti-CHCHD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHCHD4 purchase

MBS3905908 is an antibody targeting CHCHD4. Also known as Rabbit anti-CHCHD4 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CHCHD4. It may not necessarily be applicable to this product.

NCBI GeneID

131474

NCBI Accession #

NP_653237.1

UniProt Primary Accession #

Q8N4Q1

UniProt Protein Name

Mitochondrial intermembrane space import and assembly protein 40

UniProt Synonym Protein Names

Coiled-coil-helix-coiled-coil-helix domain-containing protein 4

Protein Family

Mitochondrial intermembrane space import and assembly protein

UniProt Gene Name

CHCHD4

UniProt Synonym Gene Names

MIA40

UniProt Entry Name

MIA40_HUMAN

UniProt Comments for CHCHD4

CHCHD4: Required for the import and folding of small cysteine- containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Probably acts by forming a redox cycle with GFER/ERV1 that involves a disulfide relay system. Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS (Probable). 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 3p25.1

Cellular Component: mitochondrion; mitochondrial intermembrane space

Molecular Function: protein disulfide oxidoreductase activity

Biological Process: 'de novo' posttranslational protein folding; protein maturation via protein folding; cellular protein metabolic process; protein import into mitochondrial intermembrane space; protein targeting to mitochondrion

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.