Rabbit Anti-CLCNKA Polyclonal Antibody – Cat# MBS6286394

CLCNKA Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the CLCNKA antibody detected the CLCNKA protein (arrow).

CLCNKA Antibody – Rabbit Polyclonal

Name: CLCNKA Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6286394
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6286394

$1060 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CLCNKA, Polyclonal Antibody

Full Product Name

CLCNKA, NT (CLCNKA, Chloride channel protein ClC-Ka, ClC-K1) (APC)

Product Gene Name

anti-CLCNKA antibody

Product Synonym Names

CLCNKA; Chloride channel protein ClC-Ka; ClC-K1

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

Purity/Purification

Purified by Protein A Affinity Chromatography.

Preparation and Storage

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Molecular Weight

75,285 Da

Chromosome Location

Chromosome: 1; NC_000001.10 (16348486..16360545). Location: 1p36

OMIM

602024

3D Structure

ModBase 3D Structure for P51800

Immunogen

Synthetic peptide corresponding to a portion of amino acids within aa20-47 at the N-terminalfrom human CLCNKA conjugated to KLH. Cellular Location: Membrane; Multi-pass membrane protein. Tissue Location: Expressed predominantly in kidney.

Conjugate

APC

Note

Preservative Free

Special Handling

Light sensitive

Product Categories/Family for anti-CLCNKA antibody

Antibodies; Ion Channel

Applications Tested/Suitable for anti-CLCNKA antibody

Western Blot (WB), FLISA

Application Notes for anti-CLCNKA antibody

WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Product References and Citations for anti-CLCNKA antibody

Bailey, S.D., et al. Diabetes Care (2010). In press: Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Kramer, B.K., et al. Nat Clin Pract Nephrol 4(1):38-46(2008). Martinez, G.Q., et al. PLoS ONE 3 (7), E2746 (2008): Sile, S., et al. Hum. Hered. 65(1):33-46(2008).

Other Notes

Small volumes of anti-CLCNKA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CLCNKA purchase

MBS6286394 is an antibody targeting CLCNKA. Also known as CLCNKA, NT (CLCNKA, Chloride channel protein ClC-Ka, ClC-K1) (APC). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CLCNKA. It may not necessarily be applicable to this product.

NCBI GI #

112382222

NCBI GeneID

1187

NCBI Accession #

NP_001036169.1

NCBI Related Accession #

NP_004061.3

UniProt Primary Accession #

P51800

UniProt Secondary Accession #

Q5T5P8; Q5T5Q4; Q7Z6D1; Q86VT1; B4DPD3; E7EPH6

UniProt Related Accession #

P51800

GenBank Nucleotide #

NM_001042704.1

NCBI Official Full Name

chloride channel protein ClC-Ka isoform 2

NCBI Official Synonym Full Names

chloride channel, voltage-sensitive Ka

NCBI Official Symbol

CLCNKA

NCBI Official Synonym Symbols

CLCK1; ClC-K1; hClC-Ka

UniProt Protein Name

Chloride channel protein ClC-Ka

UniProt Synonym Protein Names

ClC-K1

Protein Family

Chloride channel protein

UniProt Gene Name

CLCNKA

UniProt Synonym Gene Names

Chloride channel Ka

UniProt Entry Name

CLCKA_HUMAN

NCBI Protein Information

chloride channel protein ClC-Ka; chloride channel Ka; chloride channel, kidney, A

NCBI Summary for CLCNKA

This gene is a member of the CLC family of voltage-gated chloride channels. The encoded protein is predicted to have 12 transmembrane domains, and requires a beta subunit called barttin to form a functional channel. It is thought to function in salt reabsorption in the kidney and potassium recycling in the inner ear. The gene is highly similar to CLCNKB, which is located 10 kb downstream from this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for CLCNKA

CLCNKA: Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms. Defects in CLCNKA are a cause of Bartter syndrome type 4B (BS4B). A digenic, recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. Bartter syndrome type 4B is associated with sensorineural deafness. Belongs to the chloride channel (TC 2.A.49) family. CLCNKA subfamily.

Protein type: Membrane protein, integral; Transporter; Membrane protein, multi-pass; Transporter, ion channel

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: metal ion binding; voltage-gated chloride channel activity

Biological Process: transport; excretion; transmembrane transport

Disease: Bartter Syndrome, Type 4b

Research Articles on CLCNKA

1. The variant CLCNKA risk allele, telegraphed by linked variants in the adjacent HSPB7 gene, uncovers a previously overlooked genetic mechanism affecting the cardio-renal axis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CLCNKA antibody

Products by Pathway	Pathway Diagram
Ion Channel Transport Pathway antibodies	Ion Channel Transport Pathway Diagram
Stimuli-sensing Channels Pathway antibodies	Stimuli-sensing Channels Pathway Diagram
Transmembrane Transport Of Small Molecules Pathway antibodies	Transmembrane Transport Of Small Molecules Pathway Diagram

Diseases associated with anti-CLCNKA antibody

Disease Name	Pubmed Publications
Hypertension Antibodies	>7 publications with CLCNKA and Hypertension
Ear Diseases Antibodies	>4 publications with CLCNKA and Ear Diseases
Bone Diseases Antibodies	>1 publications with CLCNKA and Bone Diseases
Atrophy Antibodies	>1 publications with CLCNKA and Atrophy
Liver Diseases Antibodies	>1 publications with CLCNKA and Liver Diseases

Organs/Tissues associated with anti-CLCNKA antibody

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>57 publications with CLCNKA and Kidney
Lung Antibodies	>1 publications with CLCNKA and Lung