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CLDN2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6286462

MBS6286462 is an antibody targeting CLDN2. Also known as CLDN2, CT (Y195) (CLDN2, Claudin-2, SP82) (FITC). Reactive in Human. Host: Rabbit. More details

$1120 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CLDN2, Polyclonal Antibody
Full Product Name
CLDN2, CT (Y195) (CLDN2, Claudin-2, SP82) (FITC)
Product Gene Name
Product Synonym Names
CLDN2; Claudin-2; SP82
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein Isothiocyanate (FITC).
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Specificity
Recognizes human CLDN2.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
24,549 Da
OMIM
300520
3D Structure
ModBase 3D Structure for P57739
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 174-201 in the C-terminal region of human CLDN2.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-CLDN2 antibody

Applications Tested/Suitable for anti-CLDN2 antibody
Immunohistochemistry (IHC) Paraffin, FLISA, Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes for anti-CLDN2 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-CLDN2 antibody

Western Blot analysis of CLDN2 (arrow) usingMBS641972. Lane 1: 293 cell lysates (2ug/lane) Lane 2: transiently transfected with the CLDN2 gene.
Rabbit Anti-CLDN2 Polyclonal Antibody – Cat# MBS6286462

Immunohistochemistry (IHC) of anti-CLDN2 antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue using MBS641972 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS641972 for immunohistochemistry.
Rabbit Anti-CLDN2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6286462

Flow Cytometry (FC/FACS) of anti-CLDN2 antibody

Flow Cytometry analysis of 293 cells using MBS641972 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CLDN2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6286462

Product References and Citations for anti-CLDN2 antibody

1. Smith, A.J., et al. J. Acquir. Immune Defic. Syndr. 55(3):306-315(2010). 2. Kojima, F., et al. Oncol. Rep. 23(4):927-931(2010). 3. Szakal, D.N., et al. Virchows Arch. 456(3):245-250(2010). 4. Buchert, M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(6):2628-2633(2010). 5. Mankertz, J., et al. Cell Tissue Res. 336(1):67-77(2009).

Other Notes

Small volumes of anti-CLDN2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CLDN2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
claudin-2
NCBI Official Synonym Full Names
claudin 2
NCBI Official Symbol
CLDN2
UniProt Protein Name
Claudin-2
UniProt Synonym Protein Names
SP82
Protein Family
UniProt Gene Name
CLDN2
UniProt Entry Name
CLD2_HUMAN
NCBI Protein Information
claudin-2

NCBI Summary for CLDN2

This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.[provided by RefSeq, Jan 2010]
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UniProt Comments for CLDN2

Claudin-2: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Belongs to the claudin family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cytoskeletal

Chromosomal Location of Human Ortholog: Xq22.3-q23

Cellular Component: tight junction; plasma membrane; integral to membrane

Molecular Function: identical protein binding; structural molecule activity

Biological Process: calcium-independent cell-cell adhesion
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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