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CNOT8 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6467843

MBS6467843 is an antibody targeting CNOT8. Also known as CNOT8, CT (CCR4-NOT Transcription Complex Subunit 8, CCR4-associated Factor 8, CAF1-like Protein, CALIFp, CAF2, POP2, CALIF) (FITC). Reactive in Human. Host: Rabbit. More details

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Product Overview

Online Datasheet in PDF
Product Name
CNOT8, Polyclonal Antibody
Full Product Name
CNOT8, CT (CCR4-NOT Transcription Complex Subunit 8, CCR4-associated Factor 8, CAF1-like Protein, CALIFp, CAF2, POP2, CALIF) (FITC)
Product Gene Name
anti-CNOT8 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Purity/Purification
Purified by Immunoaffinity chromatography.
Specificity
Recognizes human CNOT8.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
35.9 kDa (315aa), confirmed by MALDI-TOF
OMIM
603731
3D Structure
ModBase 3D Structure for Q9UFF9
Immunogen
Synthetic peptide selected from the C-terminal region of human CNOT8 (KLH).
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-CNOT8 antibody

Antibodies; Transcription Factors
Applications Tested/Suitable for anti-CNOT8 antibody
Western Blot (WB), FLISA
Application Notes for anti-CNOT8 antibody
WB: 1:100-1:500
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-CNOT8 antibody

CNOT8 Antibody (C-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the CNOT8 antibody detected the CNOT8 protein (arrow).
Rabbit Anti-CNOT8 Polyclonal Antibody – Cat# MBS6467843

Immunofluorescence (IF) of anti-CNOT8 antibody

Fluorescent confocal image of Hela cell stained with CNOT8 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CNOT8 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CNOT8 immunoreactivity is localized to Cytoplasm significantly and Nucleus weakly.
Rabbit Anti-CNOT8 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6467843

Immunohistochemistry (IHC) of anti-CNOT8 antibody

Formalin-fixed and paraffin-embedded human colon carcinoma with CNOT8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-CNOT8 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6467843

Flow Cytometry (FC/FACS) of anti-CNOT8 antibody

Flow cytometric analysis of CEM cells using CNOT8 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CNOT8 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6467843

Product References and Citations for anti-CNOT8 antibody

1. Morel, A.P., J. Cell. Sci. 116(PT 14), 2929-2936 (2003). 2. Prevot, D., J. Biol. Chem. 276(13), 9640-9648 (2001).

Other Notes

Small volumes of anti-CNOT8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CNOT8. It may not necessarily be applicable to this product.

NCBI GI #
31542315
NCBI GeneID
9337
NCBI Accession #
NP_004770
UniProt Primary Accession #
Q9UFF9
UniProt Secondary Accession #
O95709; Q7Z521; Q9H6Y1; B0AZS3; B2RAR8; B7Z8R1; D3DQI8
UniProt Related Accession #
Q9UFF9
GenBank Nucleotide #
NM_004779.5
NCBI Official Full Name
CCR4-NOT transcription complex subunit 8 isoform 1
NCBI Official Synonym Full Names
CCR4-NOT transcription complex subunit 8
NCBI Official Symbol
CNOT8
NCBI Official Synonym Symbols
CAF1; POP2; CALIF; Caf1b; hCAF1
UniProt Protein Name
CCR4-NOT transcription complex subunit 8
UniProt Synonym Protein Names
CAF1-like protein; CALIFp; CAF2; CCR4-associated factor 8; Caf1b
Protein Family
CCR4-NOT transcription complex
UniProt Gene Name
CNOT8
UniProt Synonym Gene Names
CALIF; POP2; CALIFp
UniProt Entry Name
CNOT8_HUMAN
NCBI Protein Information
CCR4-NOT transcription complex subunit 8

UniProt Comments for CNOT8

CNOT8: Ubiquitous transcription factor required for a diverse set of processes. The CCR4-NOT complex functions as general transcription regulation complex. Belongs to the CAF1 family.

Protein type: EC 3.1.13.4

Chromosomal Location of Human Ortholog: 5q31-q33

Cellular Component: intracellular; cytosol; nucleus; CCR4-NOT complex

Molecular Function: protein binding; 3'-5'-exoribonuclease activity; poly(A)-specific ribonuclease activity; RNA binding; metal ion binding; transcription factor activity

Biological Process: regulation of translation; negative regulation of cell proliferation; poly(A) tail shortening; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of cell proliferation; gene expression; miRNA-mediated gene silencing; mRNA catabolic process, deadenylation-dependent decay
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Research Articles on CNOT8

1. These results indicate that Caf1 and Ccr4 cooperate in mRNA deadenylation and suggest that the enzyme activities of Caf1 and Ccr4 are regulated via allosteric interactions within the nuclease module.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CNOT8 antibody

 Reference Product  PubMed Publications
 BTG1 antibody  >7 publications with CNOT8 and BTG1
 CNOT1 antibody  >7 publications with CNOT8 and CNOT1
 BTG2 antibody  >6 publications with CNOT8 and BTG2
 CNOT6L antibody  >5 publications with CNOT8 and CNOT6L
 CNOT6 antibody  >5 publications with CNOT8 and CNOT6
 CNOT3 antibody  >3 publications with CNOT8 and CNOT3
 CNOT10 antibody  >1 publications with CNOT8 and CNOT10
 CNOT4 antibody  >1 publications with CNOT8 and CNOT4
 CNOT2 antibody  >1 publications with CNOT8 and CNOT2

Pathways associated with anti-CNOT8 antibody

 Products by Pathway  Pathway Diagram
 Deadenylation Of MRNA Pathway antibodies  Deadenylation Of MRNA Pathway Diagram
 Deadenylation-dependent MRNA Decay Pathway antibodies  Deadenylation-dependent MRNA Decay Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 RNA Degradation Pathway antibodies  RNA Degradation Pathway Diagram
 RNA Degradation Pathway antibodies  RNA Degradation Pathway Diagram
 TP53 Regulates Transcription Of Cell Cycle Genes Pathway antibodies  TP53 Regulates Transcription Of Cell Cycle Genes Pathway Diagram
 TP53 Regulates Transcription Of Additional Cell Cycle Genes Whose Exact Role In The P53 Pathway Remain Uncertain antibodies  TP53 Regulates Transcription Of Additional Cell Cycle Genes Whose Exact Role In The P53 Pathway Remain Uncertain Diagram
 Transcriptional Regulation By TP53 Pathway antibodies  Transcriptional Regulation By TP53 Pathway Diagram

Diseases associated with anti-CNOT8 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2 publications with CNOT8 and Inflammation
 Necrosis Antibodies  >2 publications with CNOT8 and Necrosis
 Genital Diseases, Male Antibodies  >1 publications with CNOT8 and Genital Diseases, Male

Organs/Tissues associated with anti-CNOT8 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with CNOT8 and Brain
 Nerve Antibodies  >1 publications with CNOT8 and Nerve
 Kidney Antibodies  >1 publications with CNOT8 and Kidney
 Eye Antibodies  >1 publications with CNOT8 and Eye
 Vascular Antibodies  >1 publications with CNOT8 and Vascular
 Muscle Antibodies  >1 publications with CNOT8 and Muscle
 Blood Antibodies  >1 publications with CNOT8 and Blood
 Testis Antibodies  >1 publications with CNOT8 and Testis
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