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CPN2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6467129

MBS6467129 is an antibody targeting CPN2. Also known as CPN2, NT (Carboxypeptidase N Subunit 2, Carboxypeptidase N Polypeptide 2, Carboxypeptidase N 83kD Chain, Carboxypeptidase N Regulatory Subunit, Carboxypeptidase N Large Subunit, ACBP) (PE). Reactive in… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CPN2, Polyclonal Antibody
Full Product Name
CPN2, NT (Carboxypeptidase N Subunit 2, Carboxypeptidase N Polypeptide 2, Carboxypeptidase N 83kD Chain, Carboxypeptidase N Regulatory Subunit, Carboxypeptidase N Large Subunit, ACBP) (PE)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human CPN2.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
60kDa
OMIM
603104
3D Structure
ModBase 3D Structure for P22792
Immunogen
Synthetic peptide selected from the N-terminal region of human CPN2 (KLH).
Conjugate
PE
Note
Preservative Free

Product Categories/Family for anti-CPN2 antibody

Applications Tested/Suitable for anti-CPN2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-CPN2 antibody
WB: 1:50-1:100
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-CPN2 antibody

Western blot analysis of CPN2 antibody (N-term) in jurkat cell line lysates (35ug/lane). CPN2 (arrow) was detected using the purified Pab.
Rabbit Anti-CPN2 Polyclonal Antibody – Cat# MBS6467129

Immunohistochemistry (IHC) of anti-CPN2 antibody

CPN2 Antibody (N-term) (RB18703) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CPN2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-CPN2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6467129

Flow Cytometry (FC/FACS) of anti-CPN2 antibody

CPN2 Antibody (N-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CPN2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6467129

Product References and Citations for anti-CPN2 antibody

1. Liu, T., Qian, W.J. J. Proteome Res. 4(6), 2070-2080 (2005). 2. Riley, D.A., Tan, F. Genomics 50(1), 105-108 (1998). 3. Skidgel, R.A. Biochem. Biophys. Res. Commun. 154(3), 1323-1329 (1988).

Other Notes

Small volumes of anti-CPN2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CPN2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
carboxypeptidase N subunit 2
NCBI Official Synonym Full Names
carboxypeptidase N subunit 2
NCBI Official Symbol
CPN2
NCBI Official Synonym Symbols
ACBP
UniProt Protein Name
Carboxypeptidase N subunit 2
UniProt Synonym Protein Names
Carboxypeptidase N 83 kDa chain; Carboxypeptidase N large subunit; Carboxypeptidase N polypeptide 2; Carboxypeptidase N regulatory subunit
Protein Family
UniProt Gene Name
CPN2
UniProt Synonym Gene Names
ACBP
UniProt Entry Name
CPN2_HUMAN
NCBI Protein Information
carboxypeptidase N subunit 2

UniProt Comments for CPN2

CPN2: The 83 kDa subunit binds and stabilizes the catalytic subunit at 37 degrees Celsius and keeps it in circulation. Under some circumstances it may be an allosteric modifier of the catalytic subunit.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: extracellular region

Molecular Function: enzyme regulator activity

Biological Process: protein stabilization; regulation of catalytic activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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