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CPSF7 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6288471

CPSF7 is probable a component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. Binds to cleavage and polyadenylation RNA substrates. More details

$1060 / 0.2 mL +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CPSF7, Polyclonal Antibody
Full Product Name
CPSF7, CT (CPSF7, Cleavage and polyadenylation specificity factor subunit 7, Cleavage and polyadenylation specificity factor 59kD subunit, Pre-mRNA cleavage factor Im 59kD subunit) (PE)
Product Gene Name
anti-CPSF7 antibody
Product Synonym Names
CPSF7; Cleavage and polyadenylation specificity factor subunit 7; Cleavage and polyadenylation specificity factor 59kD subunit; Pre-mRNA cleavage factor Im 59kD subunit
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
56,375 Da
3D Structure
ModBase 3D Structure for Q8N684
Immunogen
CPSF7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 434-463 amino acids from the C-terminal region of human CPSF7.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-CPSF7 antibody

Antibodies; Proteins
Applications Tested/Suitable for anti-CPSF7 antibody
Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
Application Notes for anti-CPSF7 antibody
FC/FACS: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-CPSF7 antibody

CPSF7 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CPSF7 antibody detected the CPSF7 protein (arrow).
Rabbit Anti-CPSF7 Polyclonal Antibody – Cat# MBS6288471

Flow Cytometry (FC/FACS) of anti-CPSF7 antibody

CPSF7 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CPSF7 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6288471

Product References and Citations for anti-CPSF7 antibody

Olsen, J.V., et al. Cell 127(3):635-648(2006). Lim, J., et al. Cell 125(4):801-814(2006). Brill, L.M., et al. Anal. Chem. 76(10):2763-2772(2004). Zhou, Z., et al. Nature 419(6903):182-185(2002). Ruegsegger, U., et al. J. Biol. Chem. 271(11):6107-6113(1996).

Other Notes

Small volumes of anti-CPSF7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CPSF7 purchase

MBS6288471 is an antibody targeting CPSF7. Also known as CPSF7, CT (CPSF7, Cleavage and polyadenylation specificity factor subunit 7, Cleavage and polyadenylation specificity factor 59kD subunit, Pre-mRNA cleavage factor Im 59kD subunit) (PE). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CPSF7. It may not necessarily be applicable to this product.

NCBI GI #
209862881
NCBI GeneID
79869
NCBI Accession #
NP_001129512.1
NCBI Related Accession #
NP_001136037.1; NP_079087.3
UniProt Primary Accession #
Q8N684
UniProt Secondary Accession #
Q7Z3H9; Q9H025; Q9H9V1; B3KU04; C9K0Q4
UniProt Related Accession #
Q8N684
GenBank Nucleotide #
NM_001136040.2
NCBI Official Full Name
cleavage and polyadenylation specificity factor subunit 7 isoform 2
NCBI Official Synonym Full Names
cleavage and polyadenylation specific factor 7
NCBI Official Symbol
CPSF7
NCBI Official Synonym Symbols
CFIm59
UniProt Protein Name
Cleavage and polyadenylation specificity factor subunit 7
UniProt Synonym Protein Names
Cleavage and polyadenylation specificity factor 59 kDa subunit; CFIm59; CPSF 59 kDa subunit; Pre-mRNA cleavage factor Im 59 kDa subunit
Protein Family
Cleavage and polyadenylation specificity factor
UniProt Gene Name
CPSF7
UniProt Synonym Gene Names
CFIm59; CPSF 59 kDa subunit
UniProt Entry Name
CPSF7_HUMAN
NCBI Protein Information
cleavage and polyadenylation specificity factor subunit 7

NCBI Summary for CPSF7

Cleavage factor Im (CFIm) is one of six factors necessary for correct cleavage and polyadenylation of pre-mRNAs. CFIm is composed of three different subunits of 25, 59, and 68 kDa, and it functions as a heterotetramer, with a dimer of the 25 kDa subunit binding to two of the 59 or 68 kDa subunits. The protein encoded by this gene represents the 59 kDa subunit, which can interact with the splicing factor U2 snRNP Auxiliary Factor (U2AF) 65 to link the splicing and polyadenylation complexes. [provided by RefSeq, Oct 2016]
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UniProt Comments for CPSF7

CPSF7: probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. Binds to cleavage and polyadenylation RNA substrates. Probable component of the cleavage factor Im (CFIm) complex, composed at least of NUDT21/CPSF5 and CPSF6 or CPSF7. Two alternatively-spliced isoforms have been described.

Protein type: RNA splicing; RNA processing; RNA-binding

Chromosomal Location of Human Ortholog: 11q12.2

Cellular Component: nucleoplasm; membrane; nucleus; mRNA cleavage factor complex

Molecular Function: protein binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription; protein tetramerization
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Research Articles on CPSF7

1. Data provide evidence that CFIm exists as a heterotetramer of 25-kD, 59-kD and 68-kD subunits of CFIm: CFIm25, CFIm59 and CFIm68.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CPSF7 antibody

 Products by Pathway  Pathway Diagram
 Cleavage Of Growing Transcript In The Termination Region Pathway antibodies  Cleavage Of Growing Transcript In The Termination Region Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 Processing Of Capped Intronless Pre-mRNA Pathway antibodies  Processing Of Capped Intronless Pre-mRNA Pathway Diagram
 Processing Of Intronless Pre-mRNAs Pathway antibodies  Processing Of Intronless Pre-mRNAs Pathway Diagram
 RNA Polymerase II Transcription Pathway antibodies  RNA Polymerase II Transcription Pathway Diagram
 RNA Polymerase II Transcription Termination Pathway antibodies  RNA Polymerase II Transcription Termination Pathway Diagram
 MRNA 3'-end Processing Pathway antibodies  MRNA 3'-end Processing Pathway Diagram
 MRNA Splicing Pathway antibodies  MRNA Splicing Pathway Diagram
 MRNA Splicing - Major Pathway antibodies  MRNA Splicing - Major Pathway Diagram
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