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CYP8B1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6471378

MBS6471378 is an antibody targeting CYP8B1. Also known as CYP8B1, CT (Sterol 12-alpha-hydroxylase, 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase, CYPVIIIB1, Cytochrome P450 8B1, Sterol 12-alpha-hydroxylase, 7-alpha-hydroxycholest-4-en-3-one… More details

$1060 / 0.1 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CYP8B1, Polyclonal Antibody
Full Product Name
CYP8B1, CT (Sterol 12-alpha-hydroxylase, 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase, CYPVIIIB1, Cytochrome P450 8B1, Sterol 12-alpha-hydroxylase, 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase, CYP12) (MaxLight 490)
Product Gene Name
anti-CYP8B1 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human CYP8B1.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
58kDa
OMIM
602172
3D Structure
ModBase 3D Structure for Q9UNU6
Immunogen
Synthetic peptide selected from the C-terminal region of human CYP8B1 (KLH).
Conjugate
MaxLight490
Note
Preservative Free

Product Categories/Family for anti-CYP8B1 antibody

Antibodies; Enzymes; Cytochrome
Applications Tested/Suitable for anti-CYP8B1 antibody
Western Blot (WB), FLISA
Application Notes for anti-CYP8B1 antibody
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-CYP8B1 antibody

Western blot analysis of CYP8B1 Antibody (C-term) in K562 cell line lysates (35ug/lane). CYP8B1 (arrow) was detected using the purified Pab.
Rabbit Anti-CYP8B1 Polyclonal Antibody – Cat# MBS6471378

Immunohistochemistry (IHC) of anti-CYP8B1 antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CYP8B1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-CYP8B1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6471378

Flow Cytometry (FC/FACS) of anti-CYP8B1 antibody

CYP8B1 Antibody (C-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CYP8B1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6471378

Product References and Citations for anti-CYP8B1 antibody

1. Zhang, M. et.al., J. Biol. Chem. 276(45), 41690-41699 (2001). 2. Wang, J., et.al., Histochem. Cell Biol. 123(4-5), 441-446 (2005).

Other Notes

Small volumes of anti-CYP8B1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CYP8B1. It may not necessarily be applicable to this product.

NCBI GI #
166362724
NCBI GeneID
1582
NCBI Accession #
NP_004382
UniProt Primary Accession #
Q9UNU6
UniProt Related Accession #
Q9UNU6
GenBank Nucleotide #
NM_004391
NCBI Official Full Name
7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase
NCBI Official Synonym Full Names
cytochrome P450 family 8 subfamily B member 1
NCBI Official Symbol
CYP8B1
NCBI Official Synonym Symbols
CP8B; CYP12
UniProt Protein Name
7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase
UniProt Synonym Protein Names
7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; CYPVIIIB1; Cytochrome P450 8B1; Sterol 12-alpha-hydroxylase
Protein Family
5-beta-cholestane-3-alpha,7-alpha-diol 12-alpha-hydroxylase
UniProt Gene Name
CYP8B1
UniProt Synonym Gene Names
CYP12
UniProt Entry Name
CP8B1_HUMAN
NCBI Protein Information
7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase

NCBI Summary for CYP8B1

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless. [provided by RefSeq, Jul 2008]
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UniProt Comments for CYP8B1

CYP8B1: Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha- hydroxylated C27 steroids. Belongs to the cytochrome P450 family.

Protein type: Membrane protein, integral; Oxidoreductase; EC 1.14.13.95; Lipid Metabolism - primary bile acid biosynthesis

Chromosomal Location of Human Ortholog: 3p22.1

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: sterol 12-alpha-hydroxylase activity; iron ion binding; 7alpha-hydroxycholest-4-en-3-one 12alpha-hydroxylase activity; heme binding; oxygen binding

Biological Process: bile acid biosynthetic process; bile acid metabolic process; xenobiotic metabolic process; lipid metabolic process; sterol metabolic process
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Research Articles on CYP8B1

1. RORalpha is a key regulator of diurnal rhythm and fasting induction of CYP8B1, which regulates bile acid composition and serum and liver cholesterol levels.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CYP8B1 antibody

 Products by Pathway  Pathway Diagram
 Bile Acid And Bile Salt Metabolism Pathway antibodies  Bile Acid And Bile Salt Metabolism Pathway Diagram
 Bile Acid Biosynthesis, Cholesterol => Cholate Pathway antibodies  Bile Acid Biosynthesis, Cholesterol => Cholate Pathway Diagram
 Bile Acid Biosynthesis, Cholesterol => Cholate Pathway antibodies  Bile Acid Biosynthesis, Cholesterol => Cholate Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Cytochrome P450 - Arranged By Substrate Type Pathway antibodies  Cytochrome P450 - Arranged By Substrate Type Pathway Diagram
 Endogenous Sterols Pathway antibodies  Endogenous Sterols Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Nuclear Receptors In Lipid Metabolism And Toxicity Pathway antibodies  Nuclear Receptors In Lipid Metabolism And Toxicity Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram

Diseases associated with anti-CYP8B1 antibody

 Disease Name  Pubmed Publications
 Cholestasis Antibodies  >22 publications with CYP8B1 and Cholestasis
 Liver Diseases Antibodies  >20 publications with CYP8B1 and Liver Diseases
 Disease Models, Animal Antibodies  >11 publications with CYP8B1 and Disease Models, Animal
 Drug Toxicity Antibodies  >9 publications with CYP8B1 and Drug Toxicity
 Fatty Liver Antibodies  >8 publications with CYP8B1 and Fatty Liver
 Liver Neoplasms Antibodies  >7 publications with CYP8B1 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >6 publications with CYP8B1 and Carcinoma, Hepatocellular
 Atherosclerosis Antibodies  >5 publications with CYP8B1 and Atherosclerosis
 Inflammation Antibodies  >4 publications with CYP8B1 and Inflammation
 Necrosis Antibodies  >4 publications with CYP8B1 and Necrosis

Organs/Tissues associated with anti-CYP8B1 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >124 publications with CYP8B1 and Liver
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