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DAP Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9208502

Negative regulator of autophagy. Involved in mediating interferon-gamma-induced cell death. More details

$240 / 0.08 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
DAP, Polyclonal Antibody
Full Product Name
DAP Antibody (N-term)
Product Gene Name
Product Synonym Names
Death-associated protein 1; DAP-1; DAP; DAP1
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Concentration
Vial Concentration: 0.37 (lot specific)
Specificity
This DAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-38 amino acids from the N-terminal region of human DAP.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
DAP peptide (MBS9229104) is used for blocking the activity of DAP antibody (MBS9208502)
Molecular Weight
11165
Immunogen Sequence Positions
10-38
OMIM
600954
3D Structure
ModBase 3D Structure for P51397
Antigen Type
Synthetic Peptide
Antigen Source
Human

Product Categories/Family for anti-DAP antibody

Applications Tested/Suitable for anti-DAP antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-DAP antibody
WB~~1:1000

Western Blot (WB) of anti-DAP antibody

DAP Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the DAP antibody detected the DAP protein (arrow).
Rabbit Anti-DAP Polyclonal Antibody – Cat# MBS9208502

Product References and Citations for anti-DAP antibody

Gauci S., et al. Anal. Chem. 81:4493-4501(2009).
Koren I., et al. Curr. Biol. 20:1093-1098(2010).
Deiss L.P., et al. Genes Dev. 9:15-30(1995).
Ebert L., et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Zougman A., et al. J. Proteome Res. 5:925-934(2006).

Other Notes

Small volumes of anti-DAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DAP purchase

MBS9208502 is an antibody targeting DAP. Also known as DAP Antibody (N-term). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DAP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
death-associated protein 1 isoform 2
NCBI Official Synonym Full Names
death-associated protein
NCBI Official Symbol
DAP
UniProt Protein Name
Death-associated protein 1
UniProt Gene Name
DAP
UniProt Synonym Gene Names
DAP1; DAP-1
UniProt Entry Name
DAP1_HUMAN
NCBI Protein Information
death-associated protein 1

NCBI Summary for DAP

This gene encodes a basic, proline-rich, 15-kD protein. The protein acts as a positive mediator of programmed cell death that is induced by interferon-gamma. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

UniProt Comments for DAP

DAP: a 15 kDa, proline rich protein that possesses one SH3 binding motif. Negatively regulates autophagy and is involved in mediating interferon-gamma-induced cell death. Localizes in the cytoplasm, but the detailed mechanism of its proapoptotic function is unclear.

Protein type: Autophagy

Chromosomal Location of Human Ortholog: 5p15.2

Biological Process: caspase activation; negative regulation of autophagy; inhibition of NF-kappaB transcription factor; cellular response to amino acid starvation; apoptosis; autophagy; negative regulation of transcription, DNA-dependent
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-DAP antibody

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