Rabbit Anti-DARC Polyclonal Antibody – Cat# MBS6471889

IHC staining of mouse brain usingMBS619803.

DARC Antibody – Rabbit Polyclonal

Name: DARC Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6471889
Price: 1095 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6471889

MBS6471889 is an antibody targeting DARC. Also known as DARC (CT) (Duffy Antigen/Chemokine Receptor, Duffy Antigen/Chemokine Receptor, Fy Glycoprotein D, GpFy, Cell Adhesion Molecule 3, CADM3, Plasmodium Vivax Receptor,CCBP1, CD234, CD234 antigen, Dfy, GpFy,… More details

$1095 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DARC, Polyclonal Antibody

Full Product Name

DARC (CT) (Duffy Antigen/Chemokine Receptor, Duffy Antigen/Chemokine Receptor, Fy Glycoprotein D, GpFy, Cell Adhesion Molecule 3, CADM3, Plasmodium Vivax Receptor,CCBP1, CD234, CD234 antigen, Dfy, GpFy, Plasmodium vivax receptor) (APC)

Product Gene Name

anti-DARC antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).

Purity/Purification

Purified by Immunoaffinity chromatography from whole serum.

Specificity

Recognizes human DARC. Detects bands of ~23kD and 36kD in mouse brain tissue lysate. Species Crossreactivity: mouse and rat.

Preparation and Storage

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Molecular Weight

35,553 Da

Chromosome Location

Chromosome: 1; NC_000001.10 (159173803..159176290). Location: 1q21-q22

OMIM

110700

3D Structure

ModBase 3D Structure for Q16570

Immunogen

Synthetic peptide corresponding to a 16aa sequence near the carboxy terminus of human DARC. Swiss/Uniprot Accession: Q16570.

Conjugate

APC

Note

Preservative Free

Product Categories/Family for anti-DARC antibody

Antibodies; Receptors

Applications Tested/Suitable for anti-DARC antibody

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Application Notes for anti-DARC antibody

IHC-P: Paraffin. Requires antigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate buffer pH 6.0 is recommended.
Applications are based on unconjugated antibody.

Product References and Citations for anti-DARC antibody

1. Gardner, L., et al. (2004). The human Duffy antigen binds selected inflammatory but not homeostatic chemokines. Biochem. Biophys. Res. Commun. 321:306-12. 2. Chaudhuri, A., et al. (1993) Cloning of glycoprotein D cDNA, which encodes the major subunit of the Duffy blood group system and the receptor for the Plasmodium vivax malaria parasite. PNAS 90:10793-7. 3. Gil, M.L., et al (1992) A member of the tetra spans transmembrane protein superfamily is recognized by a monoclonal antibody raised against an HLA class I-deficient, lymphokine-activated killer-susceptible, B lymphocyte line. Cloning and functional studies. J. Immunol. 2826-33. J. Immunol. 148(9):2826-33.

Other Notes

Small volumes of anti-DARC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DARC. It may not necessarily be applicable to this product.

NCBI GI #

171846251

NCBI GeneID

2532

NCBI Accession #

NP_001116423.1

UniProt Primary Accession #

Q16570

UniProt Secondary Accession #

O75898; Q16300; Q8WWE3; Q9UJP0; Q9UKZ5; Q9UKZ6; Q9UQE1; A8YPG5

UniProt Related Accession #

Q16570

GenBank Nucleotide #

NM_001122951.2

NCBI Official Full Name

Duffy antigen/chemokine receptor isoform a

NCBI Official Synonym Full Names

Duffy blood group, chemokine receptor

NCBI Official Symbol

DARC

NCBI Official Synonym Symbols

FY; Dfy; GPD; GpFy; CCBP1; CD234; WBCQ1

UniProt Protein Name

Duffy antigen/chemokine receptor

UniProt Synonym Protein Names

Fy glycoprotein; GpFy; Glycoprotein D; Plasmodium vivax receptor

Protein Family

Darcynin

UniProt Gene Name

DARC

UniProt Synonym Gene Names

FY; GPD; GpFy

UniProt Entry Name

DUFFY_HUMAN

NCBI Protein Information

Duffy antigen/chemokine receptor; glycoprotein D; Fy glycoprotein; Duffy blood group antigen; plasmodium vivax receptor

NCBI Summary for DARC

The protein encoded by this gene is a glycosylated membrane protein and a non-specific receptor for several chemokines. The encoded protein is the receptor for the human malarial parasites Plasmodium vivax and Plasmodium knowlesi. Polymorphisms in this gene are the basis of the Duffy blood group system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for DARC

DARC: Non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi. Belongs to the G-protein coupled receptor Duffy family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, Duffy family; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1q21-q22

Cellular Component: recycling endosome; early endosome; integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; transmembrane receptor activity; C-C chemokine binding; receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; defense response; inflammatory response; regulation of chemokine production

Disease: White Blood Cell Count Quantitative Trait Locus 1; Blood Group, Duffy System; Malaria, Susceptibility To

Research Articles on DARC

1. Although lower, the risk of Duffy-negatives to experience a P. vivax blood stage infection was not significantly different to that of Duffy-positives.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DARC antibody

Reference Product	PubMed Publications
CCL2 antibody	>19 publications with DARC and CCL2
CCL5 antibody	>13 publications with DARC and CCL5
CXCL1 antibody	>11 publications with DARC and CXCL1
CXCL5 antibody	>5 publications with DARC and CXCL5
IL8 antibody	>3 publications with DARC and IL8
CCL7 antibody	>1 publications with DARC and CCL7

Pathways associated with anti-DARC antibody

Products by Pathway	Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
GPCRs, Other Pathway antibodies	GPCRs, Other Pathway Diagram
Malaria Pathway antibodies	Malaria Pathway Diagram
Malaria Pathway antibodies	Malaria Pathway Diagram
Peptide GPCRs Pathway antibodies	Peptide GPCRs Pathway Diagram
Peptide Ligand-binding Receptors Pathway antibodies	Peptide Ligand-binding Receptors Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By GPCR Pathway antibodies	Signaling By GPCR Pathway Diagram

Diseases associated with anti-DARC antibody

Disease Name	Pubmed Publications
Malaria Antibodies	>68 publications with DARC and Malaria
Inflammation Antibodies	>39 publications with DARC and Inflammation
Neoplasms Antibodies	>28 publications with DARC and Neoplasms
Death Antibodies	>21 publications with DARC and Death
Necrosis Antibodies	>10 publications with DARC and Necrosis
Lung Neoplasms Antibodies	>5 publications with DARC and Lung Neoplasms
Liver Diseases Antibodies	>5 publications with DARC and Liver Diseases
Hemolysis Antibodies	>4 publications with DARC and Hemolysis
Respiratory Hypersensitivity Antibodies	>4 publications with DARC and Respiratory Hypersensitivity
Arthritis, Rheumatoid Antibodies	>3 publications with DARC and Arthritis, Rheumatoid

Organs/Tissues associated with anti-DARC antibody

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>38 publications with DARC and Vascular
Heart Antibodies	>24 publications with DARC and Heart
Lung Antibodies	>22 publications with DARC and Lung
Kidney Antibodies	>18 publications with DARC and Kidney
Brain Antibodies	>10 publications with DARC and Brain
Eye Antibodies	>7 publications with DARC and Eye
Bone Antibodies	>5 publications with DARC and Bone
Pharynx Antibodies	>3 publications with DARC and Pharynx
Placenta Antibodies	>2 publications with DARC and Placenta
Uterus Antibodies	>1 publications with DARC and Uterus