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DEK Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6291663

DEK is a protein with one SAP domain. This protein binds to cruciform and superhelical DNA and induces positive supercoils into closed circular DNA, and is also involved in splice site selection during mRNA processing. Chromosomal aberrations involving this… More details

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Product Overview

Online Datasheet in PDF
Product Name
DEK, Polyclonal Antibody
Full Product Name
DEK, CT (DEK, Protein DEK) (APC)
Product Gene Name
anti-DEK antibody
Product Synonym Names
DEK; Protein DEK
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
Predicted: 43 kDa
OMIM
125264
3D Structure
ModBase 3D Structure for P35659
Immunogen
DEK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343~372 amino acids from the C-terminal region of human DEK.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-DEK antibody

Antibodies; Proteins
Applications Tested/Suitable for anti-DEK antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-DEK antibody
FC/FACS: 1:10-50
FLISA: 1:1,000
IF: 1:10-50
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-DEK antibody

Western blot analysis of DEK Antibody (C-term) in WiDr cell line lysates (35ug/lane). DEK (arrow) was detected using the purified Pab.
Rabbit Anti-DEK Polyclonal Antibody – Cat# MBS6291663

Immunohistochemistry (IHC) of anti-DEK antibody

DEK Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DEK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-DEK Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6291663

Flow Cytometry (FC/FACS) of anti-DEK antibody

DEK Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-DEK Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6291663

Immunofluorescence (IF) of anti-DEK antibody

Confocal immunofluorescent analysis of DEK Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-DEK Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6291663

Product References and Citations for anti-DEK antibody

Sawatsubashi, S., et.al., Genes Dev. 24 (2), 159-170 (2010). Khodadoust, M.S., et.al., Cancer Res. 69 (16), 6405-6413 (2009).

Other Notes

Small volumes of anti-DEK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DEK purchase

MBS6291663 is an antibody targeting DEK. Also known as DEK, CT (DEK, Protein DEK) (APC). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DEK. It may not necessarily be applicable to this product.

NCBI GI #
544150
NCBI GeneID
7913
NCBI Accession #
P35659.1
UniProt Primary Accession #
P35659
UniProt Secondary Accession #
Q5TGV4; Q5TGV5; B2R6K6; B4DN37
UniProt Related Accession #
P35659
NCBI Official Full Name
Protein DEK
NCBI Official Synonym Full Names
DEK proto-oncogene
NCBI Official Symbol
DEK
NCBI Official Synonym Symbols
D6S231E
UniProt Protein Name
Protein DEK
Protein Family
Protein
UniProt Gene Name
DEK
UniProt Entry Name
DEK_HUMAN
NCBI Protein Information
protein DEK

NCBI Summary for DEK

This gene encodes a protein with one SAP domain. This protein binds to cruciform and superhelical DNA and induces positive supercoils into closed circular DNA, and is also involved in splice site selection during mRNA processing. Chromosomal aberrations involving this region, increased expression of this gene, and the presence of antibodies against this protein are all associated with various diseases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
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UniProt Comments for DEK

DEK: a ubiquitous protein with a SAP domain, which is found in a variety of nuclear proteins involved in transcription, DNA repair, RNA processing or apoptotic chromatin degradation. This protein binds to cruciform and superhelical DNA and induces positive supercoils into closed circular DNA and is also involved in splice site selection during mRNA processing. A chromosomal aberration involving DEK is found in a subset of acute myeloid leukemias (AML).

Protein type: Histone-binding; Oncoprotein; DNA-binding; DNA replication; RNA splicing

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: contractile fiber; nucleoplasm; nucleus

Molecular Function: DNA binding; histone binding

Biological Process: chromatin modification; positive regulation of gene expression, epigenetic; regulation of transcription from RNA polymerase II promoter; signal transduction; transcription from RNA polymerase II promoter; viral genome replication
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Research Articles on DEK

1. Results find that DEK binds to transcription start sites with a dual role in activation and repression of highly and ubiquitously expressed genes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DEK antibody

 Reference Product  PubMed Publications
 SRRM1 antibody  >4 publications with DEK and SRRM1
 RELA antibody  >3 publications with DEK and RELA
 U2AF2 antibody  >2 publications with DEK and U2AF2
 PRDX6 antibody  >1 publications with DEK and PRDX6
 TP63 antibody  >1 publications with DEK and TP63

Pathways associated with anti-DEK antibody

 Products by Pathway  Pathway Diagram
 B-WICH Complex Positively Regulates RRNA Expression Pathway antibodies  B-WICH Complex Positively Regulates RRNA Expression Pathway Diagram
 Epigenetic Regulation Of Gene Expression Pathway antibodies  Epigenetic Regulation Of Gene Expression Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Positive Epigenetic Regulation Of RRNA Expression Pathway antibodies  Positive Epigenetic Regulation Of RRNA Expression Pathway Diagram

Diseases associated with anti-DEK antibody

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >13 publications with DEK and Adenocarcinoma
 Carcinoma, Hepatocellular Antibodies  >9 publications with DEK and Carcinoma, Hepatocellular
 Breast Neoplasms Antibodies  >7 publications with DEK and Breast Neoplasms
 Lung Diseases Antibodies  >7 publications with DEK and Lung Diseases
 Cell Transformation, Neoplastic Antibodies  >6 publications with DEK and Cell Transformation, Neoplastic
 Lung Neoplasms Antibodies  >5 publications with DEK and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >5 publications with DEK and Neoplasms, Experimental
 Neoplasm Metastasis Antibodies  >5 publications with DEK and Neoplasm Metastasis
 Inflammation Antibodies  >5 publications with DEK and Inflammation
 Neoplasm Invasiveness Antibodies  >5 publications with DEK and Neoplasm Invasiveness

Organs/Tissues associated with anti-DEK antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >51 publications with DEK and Blood
 Bone Antibodies  >34 publications with DEK and Bone
 Bone Marrow Antibodies  >31 publications with DEK and Bone Marrow
 Liver Antibodies  >9 publications with DEK and Liver
 Bladder Antibodies  >9 publications with DEK and Bladder
 Eye Antibodies  >9 publications with DEK and Eye
 Lung Antibodies  >8 publications with DEK and Lung
 Skin Antibodies  >8 publications with DEK and Skin
 Brain Antibodies  >8 publications with DEK and Brain
 Lymph Node Antibodies  >6 publications with DEK and Lymph Node
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