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DFF35, 45 (DFFA) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS617288

Human DFF45 and its mouse homologue ICAD function in normal cells as chaperones for caspase-activated deoxyribonuclease (DFF40 or CAD) during its synthesis (1). The association of DFF45 (or its isoform DFF35) with DFF40 inhibits the DNAse activity of the… More details

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Product Overview

Online Datasheet in PDF
Product Name
DFF35, 45 (DFFA), Polyclonal Antibody
Full Product Name
DFF35, 45 (ICAD, DNA Fragmentation Factor, Inhibitor of Caspase Activated Deoxyribonuclease)
Product Gene Name
Product Synonym Names
Anti -DFF35, 45 (ICAD, DNA Fragmentation Factor, Inhibitor of Caspase Activated Deoxyribonuclease)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Detects endogenous levels of total full length DFF45 and/or full length DFF35. This antibody does not crossreact with related proteins.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
36,522 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (10520588..10532613, complement). Location: 1p36.3-p36.2
OMIM
601882
3D Structure
ModBase 3D Structure for O00273
Immunogen
Synthetic peptide (KLH coupled) corresponding to residues around Asp224 of human DFF45.

Product Categories/Family for anti-DFFA antibody

Applications Tested/Suitable for anti-DFFA antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-DFFA antibody
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:50

Western Blot (WB) of anti-DFFA antibody

Western Blot analysis of Jurkat cells, untreated (control) or etoposide-treated (25 uM), using DFF45/DFF35 Antibody.
Rabbit Anti-DFFA Polyclonal Antibody – Cat# MBS617288

Other Notes

Small volumes of anti-DFFA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DFFA purchase

MBS617288 is an antibody targeting DFFA. Also known as DFF35, 45 (ICAD, DNA Fragmentation Factor, Inhibitor of Caspase Activated Deoxyribonuclease). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DFFA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
DNA fragmentation factor DFF35
NCBI Official Synonym Full Names
DNA fragmentation factor, 45kDa, alpha polypeptide
NCBI Official Symbol
DFFA
NCBI Official Synonym Symbols
DFF1; ICAD; DFF-45
UniProt Protein Name
DNA fragmentation factor subunit alpha
UniProt Synonym Protein Names
DNA fragmentation factor 45 kDa subunit; DFF-45; Inhibitor of CAD
UniProt Gene Name
DFFA
UniProt Synonym Gene Names
DFF1; DFF45
UniProt Entry Name
DFFA_HUMAN
NCBI Protein Information
DNA fragmentation factor subunit alpha; DFF45; inhibitor of CAD; OTTHUMP00000001903; OTTHUMP00000001904; DNA fragmentation factor 45 kDa subunit

NCBI Summary for DFFA

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]
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UniProt Comments for DFFA

DFFA: Inhibitor of the caspase-activated DNase (DFF40). Heterodimer of DFFA and DFFB. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase

Chromosomal Location of Human Ortholog: 1p36.3-p36.2

Cellular Component: nucleoplasm; nuclear chromatin; cytoplasm; lipid particle; nucleus; cytosol

Molecular Function: protein binding

Biological Process: apoptosis; positive regulation of apoptosis; DNA fragmentation during apoptosis; cell structure disassembly during apoptosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DFFA antibody

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