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DNMT3A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6293110

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3a is a DNA… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Dnmt3a, Polyclonal Antibody
Full Product Name
Dnmt3a, NT (R46) (DNMT3A, DNA (cytosine-5)-methyltransferase 3A, DNA methyltransferase HsaIIIA) (MaxLight 550)
Product Gene Name
Product Synonym Names
DNMT3A; DNA (cytosine-5)-methyltransferase 3A; DNA methyltransferase HsaIIIA
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
102kDa
Chromosome Location
Chromosome: 2; NC_000002.11 (25451421..25565459, complement). Location: 2p23
OMIM
602769
3D Structure
ModBase 3D Structure for Q9Y6K1
Immunogen
Dnmt3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39~68 amino acids from the N-terminal region of human Dnmt3a.
Conjugate
MaxLight550
Note
Preservative Free
Applications Tested/Suitable for anti-Dnmt3a antibody
Western Blot (WB), FLISA, Immunohistochemistry (IHC)
Application Notes for anti-Dnmt3a antibody
WB: 1:50-100
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-Dnmt3a antibody

Western blot analysis of anti-Dnmt3a Antibody (N-term R46) in Jurkat cell line lysates (35ug/lane). Dnmt3a(arrow) was detected using the purified Pab.
Rabbit Anti-DNMT3A Polyclonal Antibody – Cat# MBS6293110

Immunohistochemistry (IHC) of anti-Dnmt3a antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Dnmt3a antibody (N-term R46), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-DNMT3A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6293110

Product References and Citations for anti-Dnmt3a antibody

Xie, S., et al., Gene 236(1):87-95 (1999). Robertson, K.D., et al., Nucleic Acids Res. 27(11):2291-2298 (1999).

Other Notes

Small volumes of anti-Dnmt3a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Dnmt3a purchase

MBS6293110 is an antibody targeting Dnmt3a. Also known as Dnmt3a, NT (R46) (DNMT3A, DNA (cytosine-5)-methyltransferase 3A, DNA methyltransferase HsaIIIA) (MaxLight 550). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Dnmt3a. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA (cytosine-5)-methyltransferase 3A isoform a
NCBI Official Synonym Full Names
DNA (cytosine-5-)-methyltransferase 3 alpha
NCBI Official Symbol
DNMT3A
NCBI Official Synonym Symbols
DNMT3A2; M.HsaIIIA
UniProt Protein Name
DNA (cytosine-5)-methyltransferase 3A
UniProt Synonym Protein Names
DNA methyltransferase HsaIIIA
Protein Family
UniProt Gene Name
DNMT3A
UniProt Synonym Gene Names
Dnmt3a; DNA MTase HsaIIIA; M.HsaIIIA
UniProt Entry Name
DNM3A_HUMAN
NCBI Protein Information
DNA (cytosine-5)-methyltransferase 3A; DNA (cytosine-5)-methyltransferase 3A; DNA MTase HsaIIIA; DNA cytosine methyltransferase 3A2

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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