• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

DOCK2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9608792

Function: Involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. Activates RAC1 and RAC2, but not CDC42, by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. May… More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DOCK2, Polyclonal Antibody
Full Product Name
DOCK2 Antibody
Product Gene Name
Product Synonym Names
Dedicator of cyto kinesis 2; Dedicator of cytokinesis 2; Dedicator of cytokinesis protein 2; DOCK 2; FLJ46592; KIAA0209
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
DOCK2 antibody detects endogenous levels of total DOCK2
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
DOCK2 peptide (MBS9627504) is used for blocking the activity of DOCK2 antibody (MBS9608792)
Molecular Weight
Observed: 212 kDa
Predicted: 212 kDa
3D Structure
ModBase 3D Structure for Q92608
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human DOCK2
Subcellular Location
Intracytoplasmic Membrane. Peripheral Membrane Protein. Note=Colocalizes with F-actin.
Tissue Specificity
Specifically expressed in hematopoietic cells. Highly expressed in peripheral blood leukocytes, and expressed at intermediate level in thymus and spleen. Expressed at very low level in the small intestine and colon.
Predicted Cross Reactivity
Pig, Bovine, Horse, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (86%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-DOCK2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-DOCK2 antibody
WB: 1:1000-3000
IF/ICC: 1:200-1:500

Western Blot (WB) of anti-DOCK2 antibody

Western blot analysis of DOCK2 using HepG2 whole cell lysates
Rabbit Anti-DOCK2 Polyclonal Antibody – Cat# MBS9608792

Immunofluorescene (IF) of anti-DOCK2 antibody

MBS9608792 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Rabbit Anti-DOCK2 Polyclonal Antibody, Immunofluorescene (IF) – Cat# MBS9608792

Other Notes

Small volumes of anti-DOCK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DOCK2 purchase

MBS9608792 is an antibody targeting DOCK2. Also known as DOCK2 Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DOCK2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
dedicator of cytokinesis protein 2
NCBI Official Synonym Full Names
dedicator of cytokinesis 2
NCBI Official Symbol
DOCK2
NCBI Official Synonym Symbols
IMD40
UniProt Protein Name
Dedicator of cytokinesis protein 2
UniProt Gene Name
DOCK2
UniProt Synonym Gene Names
KIAA0209
NCBI Protein Information
dedicator of cytokinesis protein 2

NCBI Summary for DOCK2

The protein encoded by this gene belongs to the CDM protein family. It is specifically expressed in hematopoietic cells and is predominantly expressed in peripheral blood leukocytes. The protein is involved in remodeling of the actin cytoskeleton required for lymphocyte migration in response to chemokine signaling. It activates members of the Rho family of GTPases, for example RAC1 and RAC2, by acting as a guanine nucleotide exchange factor (GEF) to exchange bound GDP for free GTP. Mutations in this gene result in immunodeficiency 40 (IMD40), a combined form of immunodeficiency that affects T cell number and function, also with variable defects in B cell and NK cell function. [provided by RefSeq, May 2018]
Show more

UniProt Comments for DOCK2

Involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. Activates RAC1 and RAC2, but not CDC42, by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. May also participate in IL2 transcriptional activation via the activation of RAC2.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000