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DOT1L Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3907786

DOT1L is a histone methyltransferase that methylates nucleosomal histone H3 on lysine 79. In contrast to other lysine histone methyltransferase, DOT1L does not contain a SET domain, a domain shown to be necessary for methyltransferase activity. More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
DOT1L, Polyclonal Antibody
Full Product Name
Rabbit anti-DOT1L IHC Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
H3-K79-HMTase; histone H3-K79 methyltransferase; lysine N-methyltransferase 4; KMT4; histone methyltransferase DOT1L; DOT1; DOT1-like protein; histone-lysine N-methyltransferase, H3 lysine-79 specific; DOT1-like histone methyltransferase; DOT1 like histone H3K79 methyltransferase; DOT1-like, histone H3 methyltransferase; Histone-lysine N-methyltransferase, H3 lysine-79 specific; DOT1 like histone lysine methyltransferase
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
100ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
184853 MW
OMIM
AF509504 mRNA
3D Structure
ModBase 3D Structure for Q8TEK3
Predicted Reactivity
Orangutan, Monkey, Northern white-cheeked gibbon, White-tufted-ear marmoset
Immunogen
Between 1000 and 1050
Conjugation
Unconjugated

Product Categories/Family for anti-DOT1L antibody

Applications Tested/Suitable for anti-DOT1L antibody
Immunohistochemistry (IHC)
Application Notes for anti-DOT1L antibody
IHC: 1:100-1:500

Immunohistochemistry (IHC) of anti-DOT1L antibody

Detection of human DOT1L by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-DOT1L (Cat. No. MBS3907786) used at a dilution of 1:100. Detection: DAB
Rabbit Anti-DOT1L Polyclonal Antibody – Cat# MBS3907786

Immunohistochemistry (IHC) of anti-DOT1L antibody

Detection of human DOT1L by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DOT1L (Cat. No. MBS3907786) used at a dilution of 1:100. Detection: DAB
Rabbit Anti-DOT1L Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3907786

Other Notes

Small volumes of anti-DOT1L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DOT1L purchase

MBS3907786 is an antibody targeting DOT1L. Also known as Rabbit anti-DOT1L IHC Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DOT1L. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
histone-lysine N-methyltransferase, H3 lysine-79 specific
NCBI Official Synonym Full Names
DOT1 like histone lysine methyltransferase
NCBI Official Symbol
DOT1L
NCBI Official Synonym Symbols
DOT1; KMT4
UniProt Protein Name
Histone-lysine N-methyltransferase, H3 lysine-79 specific
UniProt Synonym Protein Names
DOT1-like protein; Histone H3-K79 methyltransferase; H3-K79-HMTase; Lysine N-methyltransferase 4
UniProt Gene Name
DOT1L
UniProt Synonym Gene Names
KIAA1814; KMT4; H3-K79-HMTase
NCBI Protein Information
histone-lysine N-methyltransferase, H3 lysine-79 specific

NCBI Summary for DOT1L

The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011]

UniProt Comments for DOT1L

Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DOT1L antibody

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