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DPP3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8109122

MBS8109122 is an antibody targeting DPP3. Also known as Anti-DPP3 Antibody, Rabbit Polyclonal. Reactive in Human. Host: Rabbit. More details

$370 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DPP3, Polyclonal Antibody
Full Product Name
Anti-DPP3 Antibody, Rabbit Polyclonal
Product Gene Name
anti-DPP3 antibody
Product Synonym Names
DPP3 Antibody, Rabbit PAb, Antigen Affinity Purified; dipeptidyl-peptidase 3; Anti-DPPIII Antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Purity/Purification
Protein A & Antigen Affinity
Specificity
Human DPP3
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunogen
E Coli-derived Human DPP3 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human DPP3 fragment, and purified by antigen affinity chromatography.
Applications Tested/Suitable for anti-DPP3 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes for anti-DPP3 antibody
WB: 1:500-1:2000
IHC-P: 1:200-1:1000
IP: 0.5-2uL/mg of lysate

Western Blot (WB) of anti-DPP3 antibody

Anti-DPP3 rabbit polyclonal antibody at 1:500 dilution
Lane A: HeLa Whole Cell Lysate
Lane B: 293T Whole Cell Lysate
Lane C: Jurkat Whole Cell Lysate
Lysates/proteins at 30 ug per lane.
Secondary
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:83 kDa
Observed band size:83 kDa
Rabbit Anti-DPP3 Polyclonal Antibody – Cat# MBS8109122

Immunohistochemistry (IHC) of anti-DPP3 antibody

Immunochemical staining of human DPP3 in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.
Rabbit Anti-DPP3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS8109122

Immunoprecipitation (IP) of anti-DPP3 antibody

DPP3 was immunoprecipitated using:
Lane A:0.5 mg Jurkat Whole Cell Lysate
4 uL anti-DPP3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.
Primary antibody:
Anti-DPP3 rabbit polyclonal antibody,at 1:100 dilution
Secondary antibody:
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size :72 kDa
Rabbit Anti-DPP3 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS8109122

Other Notes

Small volumes of anti-DPP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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