Rabbit Anti-DPPA3 Polyclonal Antibody – Cat# MBS9211508

Anti-DPPA3 Antibody (C-term)at 1:1000 dilution + 293T/17 whole cell lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 18 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

DPPA3 Antibody – Rabbit Polyclonal

Name: DPPA3 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS9211508
Price: 240 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9211508

Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent… More details

$240 / 0.08 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DPPA3, Polyclonal Antibody

Full Product Name

DPPA3 Antibody (C-term)

Product Gene Name

anti-DPPA3 antibody

Product Synonym Names

Developmental pluripotency-associated protein 3; Stella-related protein; DPPA3; STELLAR

Clonality

Polyclonal

Isotype

Rabbit Ig

Host

Rabbit

Species Reactivity

Human

Form/Format

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Purity/Purification

Purified Rabbit Polyclonal Antibody (Pab)

Concentration

Vial Concentration: 0.28 (lot specific)

Specificity

This DPPA3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 145-179 amino acids from the C-terminal region of human DPPA3.

Preparation and Storage

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Antibody/Peptide Pairs

DPPA3 peptide (MBS9230057) is used for blocking the activity of DPPA3 antibody (MBS9211508)

Molecular Weight

17851

OMIM

608408

3D Structure

ModBase 3D Structure for Q6W0C5

Antigen Source

HUMAN

Product Categories/Family for anti-DPPA3 antibody

Immunology

Applications Tested/Suitable for anti-DPPA3 antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-DPPA3 antibody

WB~~1:1000

Product References and Citations for anti-DPPA3 antibody

Payer B.,et al.Curr. Biol. 13:2110-2117(2003).
Clark A.T.,et al.Stem Cells 22:169-179(2004).
Julaton V.T.,et al.Hum. Mol. Genet. 20:2238-2250(2011).
Bowles J.,et al.Cytogenet. Genome Res. 101:261-265(2003).

Other Notes

Small volumes of anti-DPPA3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DPPA3 purchase

MBS9211508 is an antibody targeting DPPA3. Also known as DPPA3 Antibody (C-term). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DPPA3. It may not necessarily be applicable to this product.

NCBI GI #

40548326

NCBI GeneID

359787

NCBI Accession #

NP_954980.1

UniProt Primary Accession #

Q6W0C5

UniProt Secondary Accession #

Q0P5U3; Q6JZS6

UniProt Related Accession #

Q6W0C5

GenBank Nucleotide #

NM_199286.3

NCBI Official Full Name

developmental pluripotency-associated protein 3

NCBI Official Synonym Full Names

developmental pluripotency associated 3

NCBI Official Symbol

DPPA3

NCBI Official Synonym Symbols

STELLA

UniProt Protein Name

Developmental pluripotency-associated protein 3

UniProt Synonym Protein Names

Stella-related protein

Protein Family

Developmental pluripotency-associated protein

UniProt Gene Name

DPPA3

UniProt Synonym Gene Names

STELLAR

UniProt Entry Name

DPPA3_HUMAN

NCBI Protein Information

developmental pluripotency-associated protein 3

NCBI Summary for DPPA3

This gene encodes a protein that in mice may function as a maternal factor during the preimplantation stage of development. In mice, this gene may play a role in transcriptional repression, cell division, and maintenance of cell pluripotentiality. In humans, related intronless loci are located on chromosomes 14 and X. [provided by RefSeq, Jul 2008]

UniProt Comments for DPPA3

DPPA3: Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis.

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: male pronucleus; cytoplasm; female pronucleus; nucleus

Molecular Function: methylated histone residue binding

Biological Process: chromatin modification; embryonic cleavage

Research Articles on DPPA3

1. a role for STELLA in facilitating germline and endodermal differentiation of human ES cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DPPA3 antibody

Reference Product	PubMed Publications
NANOG antibody	>28 publications with DPPA3 and NANOG
GDF3 antibody	>5 publications with DPPA3 and GDF3

Diseases associated with anti-DPPA3 antibody

Disease Name	Pubmed Publications
Neoplasms, Germ Cell and Embryonal Antibodies	>5 publications with DPPA3 and Neoplasms, Germ Cell and Embryonal
Hyperplasia Antibodies	>1 publications with DPPA3 and Hyperplasia
Fibrosis Antibodies	>1 publications with DPPA3 and Fibrosis
Breast Neoplasms Antibodies	>1 publications with DPPA3 and Breast Neoplasms
Pain Antibodies	>1 publications with DPPA3 and Pain
Hematologic Diseases Antibodies	>1 publications with DPPA3 and Hematologic Diseases