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EAPII Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6294141

This gene encodes a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B… More details

$1060 / 0.2 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
EAPII, Polyclonal Antibody
Full Product Name
EAPII, CT (TDP2, EAP2, TTRAP, Tyrosyl-DNA phosphodiesterase 2, 5'-tyrosyl-DNA phosphodiesterase, ETS1-associated protein 2, ETS1-associated protein II, TRAF and TNF receptor-associated protein) (Azide free) (HRP)
Product Gene Name
anti-EAPII antibody
Product Synonym Gene Name
TDP2
Product Synonym Names
TDP2; EAP2; TTRAP; Tyrosyl-DNA phosphodiesterase 2; 5'-tyrosyl-DNA phosphodiesterase; ETS1-associated protein 2; ETS1-associated protein II; TRAF and TNF receptor-associated protein
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
43.3 kDa (385aa) confirmed by MALDI-TOF
OMIM
605764
3D Structure
ModBase 3D Structure for O95551
Immunogen
EAPII antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the C-terminal region of human EAPII.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-EAPII antibody

Antibodies; Enzymes; Phosphodiesterase
Applications Tested/Suitable for anti-EAPII antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-EAPII antibody
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-EAPII antibody

EAPII Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the EAPII antibody detected the EAPII protein (arrow).
Rabbit Anti-EAPII Polyclonal Antibody – Cat# MBS6294141

Immunohistochemistry (IHC) of anti-EAPII antibody

EAPII Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EAPII Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-EAPII Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6294141

Immunofluorescence (IF) of anti-EAPII antibody

Confocal immunofluorescent analysis of EAPII Antibody (C-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-EAPII Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6294141

Flow Cytometry (FC/FACS) of anti-EAPII antibody

EAPII Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-EAPII Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6294141

Product References and Citations for anti-EAPII antibody

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). Wang, B.Y., et al. Mol. Biol. Rep. 37(6):2809-2816(2010). Iijima, M., et al. Neurology 73(17):1348-1352(2009). Cortes Ledesma, F., et al. Nature 461(7264):674-678(2009). Zhang, J.Q., et al. Biochem. Biophys. Res. Commun. 387(2):256-260(2009).

Other Notes

Small volumes of anti-EAPII antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EAPII purchase

MBS6294141 is an antibody targeting EAPII. Also known as EAPII, CT (TDP2, EAP2, TTRAP, Tyrosyl-DNA phosphodiesterase 2, 5'-tyrosyl-DNA phosphodiesterase, ETS1-associated protein 2, ETS1-associated protein II, TRAF and TNF receptor-associated protein) (Azide free) (HRP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EAPII. It may not necessarily be applicable to this product.

NCBI GI #
23510348
NCBI GeneID
51567
NCBI Accession #
NP_057698
UniProt Primary Accession #
O95551
UniProt Secondary Accession #
Q2TBE2; Q5JYM0; Q7Z6U5; Q9NUK5; Q9NYY9; B4DKL8; B4DQ95
UniProt Related Accession #
O95551
GenBank Nucleotide #
NM_016614.2
NCBI Official Full Name
tyrosyl-DNA phosphodiesterase 2
NCBI Official Synonym Full Names
tyrosyl-DNA phosphodiesterase 2
NCBI Official Symbol
TDP2
NCBI Official Synonym Symbols
EAP2; AD022; EAPII; TTRAP; hTDP2; dJ30M3.3
UniProt Protein Name
Tyrosyl-DNA phosphodiesterase 2
UniProt Synonym Protein Names
5'-tyrosyl-DNA phosphodiesterase; 5'-Tyr-DNA phosphodiesterase; ETS1-associated protein 2; ETS1-associated protein II; EAPII; TRAF and TNF receptor-associated protein; Tyrosyl-RNA phosphodiesterase; VPg unlinkase
UniProt Gene Name
TDP2
UniProt Synonym Gene Names
EAP2; TTRAP; Tyr-DNA phosphodiesterase 2; hTDP2; 5'-Tyr-DNA phosphodiesterase; EAPII
UniProt Entry Name
TYDP2_HUMAN
NCBI Protein Information
tyrosyl-DNA phosphodiesterase 2

UniProt Comments for EAPII

TTRAP: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'- phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has preference for single-stranded DNA or duplex DNA with a 4 base pair overhang as substrate. Has also 3'- tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. Constitutes the major if not only 5'- tyrosyl-DNA phosphodiesterase in cells. Also acts as a 5'-tyrosyl- RNA phosphodiesterase following picornavirus infection: its activity is hijacked by picornavirus and acts by specifically cleaving the protein-RNA covalent linkage generated during the viral genomic RNA replication steps of a picornavirus infection, without impairing the integrity of viral RNA. Also acts as an adapter by participating to the specific activation of MAP3K7/TAK1 in response to TGF-beta: associates with components of the TGF- beta receptor-TRAF6-TAK1 signaling module and promotes their ubiquitination dependent complex formation. Involved in non- canonical TGF-beta induced signaling routes. May also act as a negative regulator of ETS1 and may inhibit NF-kappa-B activation. Acts as a regulator of ribosome biogenesis following stress. Belongs to the CCR4/nocturin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Inhibitor; EC 3.1.4.-

Chromosomal Location of Human Ortholog: 6p22.3-p22.1

Cellular Component: nucleoplasm; PML body; intercellular bridge; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; nuclease activity; manganese ion binding; magnesium ion binding; transcription corepressor activity; single-stranded DNA binding

Biological Process: cell surface receptor linked signal transduction; double-strand break repair
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Research Articles on EAPII

1. TDP2 suppresses chromosome rearrangements induced by TOP2 and reduces TOP2-induced chromosome translocations that arise during gene transcription.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-EAPII antibody

 Products by Pathway  Pathway Diagram
 CD40/CD40L Signaling Pathway antibodies  CD40/CD40L Signaling Pathway Diagram
 DNA Double-Strand Break Repair Pathway antibodies  DNA Double-Strand Break Repair Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Nonhomologous End-Joining (NHEJ) Pathway antibodies  Nonhomologous End-Joining (NHEJ) Pathway Diagram

Diseases associated with anti-EAPII antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >6 publications with EAPII and Necrosis
 Lung Neoplasms Antibodies  >3 publications with EAPII and Lung Neoplasms
 Congenital Abnormalities Antibodies  >2 publications with EAPII and Congenital Abnormalities
 Neoplasm Metastasis Antibodies  >1 publications with EAPII and Neoplasm Metastasis
 Ovarian Neoplasms Antibodies  >1 publications with EAPII and Ovarian Neoplasms
 Chromosome Aberrations Antibodies  >1 publications with EAPII and Chromosome Aberrations
 Breast Neoplasms Antibodies  >1 publications with EAPII and Breast Neoplasms
 Inflammation Antibodies  >1 publications with EAPII and Inflammation
 Liver Neoplasms Antibodies  >1 publications with EAPII and Liver Neoplasms

Organs/Tissues associated with anti-EAPII antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >6 publications with EAPII and Brain
 Nerve Antibodies  >5 publications with EAPII and Nerve
 Lung Antibodies  >3 publications with EAPII and Lung
 Bone Antibodies  >2 publications with EAPII and Bone
 Mouth Antibodies  >1 publications with EAPII and Mouth
 Embryonic Tissue Antibodies  >1 publications with EAPII and Embryonic Tissue
 Liver Antibodies  >1 publications with EAPII and Liver
 Blood Antibodies  >1 publications with EAPII and Blood
 Bone Marrow Antibodies  >1 publications with EAPII and Bone Marrow
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