Rabbit Anti-EDEM3 Polyclonal Antibody – Cat# MBS6294339

EDEM3 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the EDEM3 antibody detected the EDEM3 protein (arrow).

EDEM3 Antibody – Rabbit Polyclonal

Name: EDEM3 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6294339
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6294339

Quality control in the endoplasmic reticulum (ER) ensures that only properly folded proteins are retained in the cell through recognition and degradation of misfolded or unassembled proteins. EDEM3 belongs to a group of proteins that accelerate degradation of… More details

$1060 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

EDEM3, Polyclonal Antibody

Full Product Name

EDEM3, CT (EDEM3, C1orf22, ER degradation-enhancing alpha-mannosidase-like 3) (Azide free) (HRP)

Product Gene Name

anti-EDEM3 antibody

Product Synonym Names

EDEM3; C1orf22; ER degradation-enhancing alpha-mannosidase-like 3

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

Purity/Purification

Purified by Protein A Affinity Chromatography.

Preparation and Storage

Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

104,664 Da

Chromosome Location

Chromosome: 1; NC_000001.10 (184659625..184724041, complement). Location: 1q25

OMIM

610214

3D Structure

ModBase 3D Structure for Q9BZQ6

Immunogen

EDEM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 773-802 amino acids from the C-terminal region of human EDEM3.

Conjugate

HRP

Note

Preservative Free

Product Categories/Family for anti-EDEM3 antibody

Antibodies; Enzymes; Glycosidase

Applications Tested/Suitable for anti-EDEM3 antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-EDEM3 antibody

WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Product References and Citations for anti-EDEM3 antibody

Hirao, K., et al. J. Biol. Chem. 281(14):9650-9658(2006). Mast, S.W., et al. Glycobiology 15(4):421-436(2005). Olivari, S., et al. J. Biol. Chem. 280(4):2424-2428(2005). Sood, R., et al. Genomics 73(2):211-222(2001).

Other Notes

Small volumes of anti-EDEM3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EDEM3 purchase

MBS6294339 is an antibody targeting EDEM3. Also known as EDEM3, CT (EDEM3, C1orf22, ER degradation-enhancing alpha-mannosidase-like 3) (Azide free) (HRP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EDEM3. It may not necessarily be applicable to this product.

NCBI GI #

163310725

NCBI GeneID

80267

NCBI Accession #

NP_079467.3

UniProt Primary Accession #

Q9BZQ6

UniProt Secondary Accession #

Q0VGM5; Q5TEZ0; Q9HCW1; Q9UFV7; B2RCH6

UniProt Related Accession #

Q9BZQ6

GenBank Nucleotide #

NM_025191.3

NCBI Official Full Name

ER degradation-enhancing alpha-mannosidase-like protein 3

NCBI Official Synonym Full Names

ER degradation enhancer, mannosidase alpha-like 3

NCBI Official Symbol

EDEM3

NCBI Official Synonym Symbols

C1orf22

UniProt Protein Name

ER degradation-enhancing alpha-mannosidase-like protein 3

UniProt Synonym Protein Names

Alpha-1,2-mannosidase EDEM3

Protein Family

ER degradation-enhancing alpha-mannosidase-like protein

UniProt Gene Name

EDEM3

UniProt Synonym Gene Names

C1orf22

UniProt Entry Name

EDEM3_HUMAN

NCBI Protein Information

ER degradation-enhancing alpha-mannosidase-like protein 3; alpha-1,2-mannosidase EDEM3; ER degradation-enhancing alpha-mannosidase-like 3; ER degradation-enhancing -mannosidase-like protein 3

UniProt Comments for EDEM3

Function: Involved in endoplasmic reticulum-associated degradation (ERAD). Accelerates the glycoprotein ERAD by proteasomes. This process depends on mannose-trimming from the N-glycans. Seems to have alpha 1,2-mannosidase activity

By similarity.

Catalytic activity: Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Subcellular location: Endoplasmic reticulum lumen

By similarity.

Domain: Contains a protease-associated domain (PA) of unknown function.

Sequence similarities: Belongs to the glycosyl hydrolase 47 family.Contains 1 PA (protease associated) domain.

Sequence caution: The sequence AAG60613.1 differs from that shown. Reason: Erroneous initiation. The sequence BAG37573.1 differs from that shown. Reason: Erroneous initiation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EDEM3 antibody

Reference Product	PubMed Publications
SEL1L antibody	>2 publications with EDEM3 and SEL1L
OS9 antibody	>1 publications with EDEM3 and OS9

Pathways associated with anti-EDEM3 antibody

Products by Pathway	Pathway Diagram
Asparagine N-linked Glycosylation Pathway antibodies	Asparagine N-linked Glycosylation Pathway Diagram
Calnexin/calreticulin Cycle Pathway antibodies	Calnexin/calreticulin Cycle Pathway Diagram
ER Quality Control Compartment (ERQC) Pathway antibodies	ER Quality Control Compartment (ERQC) Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
N-glycan Trimming In The ER And Calnexin/Calreticulin Cycle Pathway antibodies	N-glycan Trimming In The ER And Calnexin/Calreticulin Cycle Pathway Diagram
Post-translational Protein Modification Pathway antibodies	Post-translational Protein Modification Pathway Diagram
Protein Processing In Endoplasmic Reticulum Pathway antibodies	Protein Processing In Endoplasmic Reticulum Pathway Diagram
Protein Processing In Endoplasmic Reticulum Pathway antibodies	Protein Processing In Endoplasmic Reticulum Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By Wnt Pathway antibodies	Signaling By Wnt Pathway Diagram

Diseases associated with anti-EDEM3 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>1 publications with EDEM3 and Inflammation
Fetal Death Antibodies	>1 publications with EDEM3 and Fetal Death
Brain Diseases Antibodies	>1 publications with EDEM3 and Brain Diseases
Liver Diseases Antibodies	>1 publications with EDEM3 and Liver Diseases

Organs/Tissues associated with anti-EDEM3 antibody

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>1 publications with EDEM3 and Bone
Brain Antibodies	>1 publications with EDEM3 and Brain