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EEF2K Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9402133

This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
EEF2K, Polyclonal Antibody
Full Product Name
EEF2K antibody
Product Gene Name
anti-EEF2K antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
Purity/Purification
Purified by antigen-affinity chromatography.
Preparation and Storage
Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
Molecular Weight
82,144 Da
OMIM
606968
Immunogen Type
Recombinant protein
Immunogen Description
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 238 of EEF2K
Target Name
EEF2K

Product Categories/Family for anti-EEF2K antibody

Total protein Ab
Applications Tested/Suitable for anti-EEF2K antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-EEF2K antibody
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:50-1:500
Immunofluorescence: 1:100-1:200

Testing Data of anti-EEF2K antibody

Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 7.5% SDS PAGE Primary antibody diluted at 1: 5000
Rabbit Anti-EEF2K Polyclonal Antibody – Cat# MBS9402133

Immunohistochemistry (IHC) of anti-EEF2K antibody

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using EEF2K antibody at 1: 500 dilution.
Rabbit Anti-EEF2K Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9402133

Immunofluorescence (IF) of anti-EEF2K antibody

Immunofluorescence analysis of paraformaldehyde-fixed A431, using EEF2K antibody at 1: 200 dilution.
Rabbit Anti-EEF2K Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9402133

Other Notes

Small volumes of anti-EEF2K antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EEF2K purchase

MBS9402133 is an antibody targeting EEF2K. Also known as EEF2K antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EEF2K. It may not necessarily be applicable to this product.

NCBI GI #
9558749
NCBI GeneID
29904
NCBI Accession #
NP_037434
UniProt Secondary Accession #
Q8N588
UniProt Related Accession #
O00418
GenBank Nucleotide #
NM_013302.3
NCBI Official Full Name
eukaryotic elongation factor 2 kinase
NCBI Official Synonym Full Names
eukaryotic elongation factor 2 kinase
NCBI Official Symbol
EEF2K
NCBI Official Synonym Symbols
eEF-2K; HSU93850
UniProt Protein Name
Eukaryotic elongation factor 2 kinase
UniProt Synonym Protein Names
Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
Protein Family
Eukaryotic elongation factor 2 kinase
UniProt Gene Name
EEF2K
UniProt Synonym Gene Names
eEF-2 kinase; eEF-2K
UniProt Entry Name
EF2K_HUMAN
NCBI Protein Information
eukaryotic elongation factor 2 kinase

UniProt Comments for EEF2K

EEF2K: an atypical protein kinase of the Alpha family. Dependent on Ca2 ions and calmodulin. May play a key role linking cellular energy status and the inhibition of protein synthesis, a major consumer of metabolic energy. Phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation eEF2K is phosphorylated and activated by AMPK in response to elevated AMP levels, which increase during cellular stress and starvation. Regulated in response to a range of stimuli including TNF-alpha. Overexpressed in breast cancer cell lines and tumors. Activated in rat glioblastoma, and growth was inhibited by the drug Rottlerin. Activity induced in ischemic heart muscle

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, atypical; Translation; EC 2.7.11.20; ATYPICAL group; Alpha family; eEF2K subfamily

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: cytoplasm; cytosol

Molecular Function: calmodulin binding; elongation factor-2 kinase activity; translation factor activity, nucleic acid binding; calcium ion binding; ATP binding; protein kinase activity

Biological Process: translational elongation; regulation of protein amino acid autophosphorylation; protein amino acid autophosphorylation; insulin receptor signaling pathway
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Research Articles on EEF2K

1. Results show that eEF2K is rapidly activated in response to acidosis in cells, an effect that is followed by its downregulation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EEF2K antibody

 Reference Product  PubMed Publications
 RPS6KA1 antibody  >3 publications with EEF2K and RPS6KA1
 UBC antibody  >3 publications with EEF2K and UBC

Pathways associated with anti-EEF2K antibody

 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 IGF1R Signaling Cascade Pathway antibodies  IGF1R Signaling Cascade Pathway Diagram
 IRS-mediated Signalling Pathway antibodies  IRS-mediated Signalling Pathway Diagram
 IRS-related Events Triggered By IGF1R Pathway antibodies  IRS-related Events Triggered By IGF1R Pathway Diagram
 Insulin Receptor Signalling Cascade Pathway antibodies  Insulin Receptor Signalling Cascade Pathway Diagram
 Oxytocin Signaling Pathway antibodies  Oxytocin Signaling Pathway Diagram
 Oxytocin Signaling Pathway antibodies  Oxytocin Signaling Pathway Diagram
 PI3K Cascade Pathway antibodies  PI3K Cascade Pathway Diagram

Diseases associated with anti-EEF2K antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >7 publications with EEF2K and Nervous System Diseases
 Hypertrophy Antibodies  >7 publications with EEF2K and Hypertrophy
 Atrophy Antibodies  >5 publications with EEF2K and Atrophy
 Cardiovascular Diseases Antibodies  >5 publications with EEF2K and Cardiovascular Diseases
 Necrosis Antibodies  >5 publications with EEF2K and Necrosis
 Hypertension Antibodies  >4 publications with EEF2K and Hypertension
 Disease Models, Animal Antibodies  >4 publications with EEF2K and Disease Models, Animal
 Depressive Disorder Antibodies  >3 publications with EEF2K and Depressive Disorder
 Inflammation Antibodies  >3 publications with EEF2K and Inflammation
 Neoplasms, Experimental Antibodies  >2 publications with EEF2K and Neoplasms, Experimental

Organs/Tissues associated with anti-EEF2K antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >29 publications with EEF2K and Brain
 Muscle Antibodies  >24 publications with EEF2K and Muscle
 Heart Antibodies  >13 publications with EEF2K and Heart
 Blood Antibodies  >11 publications with EEF2K and Blood
 Liver Antibodies  >7 publications with EEF2K and Liver
 Kidney Antibodies  >5 publications with EEF2K and Kidney
 Lung Antibodies  >4 publications with EEF2K and Lung
 Pancreas Antibodies  >4 publications with EEF2K and Pancreas
 Mammary Gland Antibodies  >1 publications with EEF2K and Mammary Gland
 Skin Antibodies  >1 publications with EEF2K and Skin
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