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eIF2B5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3902924

eIF2B5 is one of five subunits of the eukaryotic translation initiation factor 2B (eIF2B). eIF2B5 functions as a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in the EIF2B5 gene and the… More details

$250 / 0.01 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
eIF2B5, Polyclonal Antibody
Full Product Name
Rabbit anti-eIF2B5 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
CACH; LVWM; eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa; EIF2Bepsilon; eIF-2B GDP-GTP exchange factor subunit epsilon; CLE; translation initiation factor eIF-2B subunit epsilon; EIF-2B; Translation initiation factor eIF-2B subunit epsilon; eukaryotic translation initiation factor 2B subunit epsilon
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
80,380 Da
OMIM
603896
3D Structure
ModBase 3D Structure for Q13144
Immunogen
Between 400 and 450
Conjugation
Unconjugated

Product Categories/Family for anti-eIF2B5 antibody

Applications Tested/Suitable for anti-eIF2B5 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-eIF2B5 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-eIF2B5 antibody

Detection of human eIF2B5 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF2B5 (Cat. No. MBS3902924) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-eIF2B5 Polyclonal Antibody – Cat# MBS3902924

Western Blot (WB) of anti-eIF2B5 antibody

Detection of human eIF2B5 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-eIF2B5 antibody MBS3902924 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. eIF2B5 was also immunoprecipitated by rabbit anti-eIF2B5 antibody MBS3902925, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Rabbit Anti-eIF2B5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3902924

Other Notes

Small volumes of anti-eIF2B5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for eIF2B5 purchase

MBS3902924 is an antibody targeting eIF2B5. Also known as Rabbit anti-eIF2B5 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for eIF2B5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
translation initiation factor eIF-2B subunit epsilon
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa
NCBI Official Symbol
EIF2B5
NCBI Official Synonym Symbols
CLE; CACH; LVWM; EIF-2B; EIF2Bepsilon
UniProt Protein Name
Translation initiation factor eIF-2B subunit epsilon
UniProt Synonym Protein Names
eIF-2B GDP-GTP exchange factor subunit epsilon
UniProt Gene Name
EIF2B5
UniProt Synonym Gene Names
EIF2BE
UniProt Entry Name
EI2BE_HUMAN
NCBI Protein Information
translation initiation factor eIF-2B subunit epsilon; eIF-2B GDP-GTP exchange factor subunit epsilon

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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