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EIF4A2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6295064

Eukaryotic initiation factor 4A plays an important role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins. Two highly homologous forms of functional EIF4A genes, Eif4a1 and Eif4a2, have been isolated in mice; yeast cells… More details

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Product Overview

Online Datasheet in PDF
Product Name
EIF4A2, Polyclonal Antibody
Full Product Name
EIF4A2, CT (EIF4A2, DDX2B, EIF4F, Eukaryotic initiation factor 4A-II, ATP-dependent RNA helicase eIF4A-2) (FITC)
Product Gene Name
anti-EIF4A2 antibody
Product Synonym Names
EIF4A2; DDX2B; EIF4F; Eukaryotic initiation factor 4A-II; ATP-dependent RNA helicase eIF4A-2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
407
OMIM
601102
3D Structure
ModBase 3D Structure for Q14240
Immunogen
EIF4A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 339~369 amino acids from the C-terminal region of human EIF4A2.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-EIF4A2 antibody

Antibodies; Translation Initiation Factors
Applications Tested/Suitable for anti-EIF4A2 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-EIF4A2 antibody
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-EIF4A2 antibody

Western blot analysis of EIF4A2 Antibody (C-term) in K562 cell line lysates (35ug/lane). EIF4A2 (arrow) was detected using the purified Pab.
Rabbit Anti-EIF4A2 Polyclonal Antibody – Cat# MBS6295064

Immunohistochemistry (IHC) of anti-EIF4A2 antibody

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF4A2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-EIF4A2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6295064

Flow Cytometry (FC/FACS) of anti-EIF4A2 antibody

EIF4A2 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-EIF4A2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6295064

Western Blot (WB) of anti-EIF4A2 antibody

Western blot analysis of EIF4A2 (arrow) using rabbit polyclonal EIF4A2 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4A2 gene.
Rabbit Anti-EIF4A2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6295064

Product References and Citations for anti-EIF4A2 antibody

Martins-de-Souza, D., et.al., J Neural Transm 116 (3), 275-289 (2009). Lin, C.J., et.al., Cancer Res. 68 (13), 5326-5334 (2008).

Other Notes

Small volumes of anti-EIF4A2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EIF4A2 purchase

MBS6295064 is an antibody targeting EIF4A2. Also known as EIF4A2, CT (EIF4A2, DDX2B, EIF4F, Eukaryotic initiation factor 4A-II, ATP-dependent RNA helicase eIF4A-2) (FITC). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EIF4A2. It may not necessarily be applicable to this product.

NCBI GI #
45645183
NCBI GeneID
1974
NCBI Accession #
Q14240.2
UniProt Primary Accession #
Q14240
UniProt Secondary Accession #
Q53XJ6; Q96B90; Q96EA8; D3DNU9
UniProt Related Accession #
Q14240
NCBI Official Full Name
Eukaryotic initiation factor 4A-II
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4A2
NCBI Official Symbol
EIF4A2
NCBI Official Synonym Symbols
DDX2B; EIF4A; EIF4F; BM-010; eIF4A-II; eIF-4A-II
UniProt Protein Name
Eukaryotic initiation factor 4A-II
UniProt Synonym Protein Names
ATP-dependent RNA helicase eIF4A-2
Protein Family
Eukaryotic initiation factor
UniProt Gene Name
EIF4A2
UniProt Synonym Gene Names
DDX2B; EIF4F; eIF-4A-II; eIF4A-II
UniProt Entry Name
IF4A2_HUMAN
NCBI Protein Information
eukaryotic initiation factor 4A-II; ATP-dependent RNA helicase eIF4A-2

UniProt Comments for EIF4A2

EIF4A2: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. Belongs to the DEAD box helicase family. eIF4A subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Translation; Translation initiation; Helicase; EC 3.6.4.13

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: perinuclear region of cytoplasm; eukaryotic translation initiation factor 4F complex; cytosol

Molecular Function: protein binding; translation initiation factor activity; ATPase activity; helicase activity; ATP binding

Biological Process: poly(A) tail shortening; cellular protein metabolic process; translation; viral reproduction; cytokine and chemokine mediated signaling pathway; translational initiation; gene expression; regulation of translational initiation; mRNA catabolic process, deadenylation-dependent decay
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Research Articles on EIF4A2

1. expression is an independent prognostic factor for patients with non-small-cell lung cancer for both overall survival and disease-free survival

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EIF4A2 antibody

 Reference Product  PubMed Publications
 EIF4G1 antibody  >1 publications with EIF4A2 and EIF4G1

Pathways associated with anti-EIF4A2 antibody

 Products by Pathway  Pathway Diagram
 Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway antibodies  Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway Diagram
 Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies  Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
 Cap-dependent Translation Initiation Pathway antibodies  Cap-dependent Translation Initiation Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Deadenylation Of MRNA Pathway antibodies  Deadenylation Of MRNA Pathway Diagram
 Deadenylation-dependent MRNA Decay Pathway antibodies  Deadenylation-dependent MRNA Decay Pathway Diagram
 Eukaryotic Translation Initiation Pathway antibodies  Eukaryotic Translation Initiation Pathway Diagram
 GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway antibodies  GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 ISG15 Antiviral Mechanism Pathway antibodies  ISG15 Antiviral Mechanism Pathway Diagram

Diseases associated with anti-EIF4A2 antibody

 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >1 publications with EIF4A2 and Lung Diseases
 Lymphoma Antibodies  >1 publications with EIF4A2 and Lymphoma
 Fibrosis Antibodies  >1 publications with EIF4A2 and Fibrosis
 Fatty Liver Antibodies  >1 publications with EIF4A2 and Fatty Liver
 Lung Neoplasms Antibodies  >1 publications with EIF4A2 and Lung Neoplasms
 Liver Diseases Antibodies  >1 publications with EIF4A2 and Liver Diseases
 Prenatal Exposure Delayed Effects Antibodies  >1 publications with EIF4A2 and Prenatal Exposure Delayed Effects

Organs/Tissues associated with anti-EIF4A2 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >3 publications with EIF4A2 and Liver
 Muscle Antibodies  >3 publications with EIF4A2 and Muscle
 Brain Antibodies  >3 publications with EIF4A2 and Brain
 Blood Antibodies  >2 publications with EIF4A2 and Blood
 Ovary Antibodies  >1 publications with EIF4A2 and Ovary
 Pancreas Antibodies  >1 publications with EIF4A2 and Pancreas
 Lung Antibodies  >1 publications with EIF4A2 and Lung
 Embryonic Tissue Antibodies  >1 publications with EIF4A2 and Embryonic Tissue
 Kidney Antibodies  >1 publications with EIF4A2 and Kidney
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