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eIF6 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3903339

Eukaryotic translation initiation factor 6 (eIF6) is insoluble and found both in the nucleus and in the cytoplasm. eIF6 can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits.eIF6 binds to the… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
eIF6, Polyclonal Antibody
Full Product Name
Rabbit anti-eIF6 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
eukaryotic translation initiation factor 3A; p27(BBP); p27 beta-4 integrin-binding protein; p27BBP; eukaryotic translation initiation factor 6; EIF3A; CAB; B4 integrin interactor; B(2)GCN homolog; b(2)gcn; ITGB4BP; eIF-6; Eukaryotic translation initiation factor 6; eukaryotic translation initiation factor 6
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
26,599 Da
Chromosome Location
Chromosome: 20; NC_000020.10 (33866709..33872619, complement). Location: 20q12
OMIM
602912
3D Structure
ModBase 3D Structure for P56537
Immunogen
Between 75 and 125
Conjugation
Unconjugated

Product Categories/Family for anti-eIF6 antibody

Applications Tested/Suitable for anti-eIF6 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-eIF6 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-eIF6 antibody

Detection of human eIF6 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF6 (Cat. No. MBS3903339 Lot1, left image and Cat. No. MBS3903340 Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Rabbit Anti-eIF6 Polyclonal Antibody – Cat# MBS3903339

Immunohistochemistry (IHC) of anti-eIF6 antibody

Detection of mouse eIF6 by immunohistochemistry. Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-eIF6 (Cat. No. MBS3903339 Lot1, left image and Cat. No. MBS3903340 Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-eIF6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3903339

Western Blot (WB) of anti-eIF6 antibody

Detection of human eIF6 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-eIF6 antibody MBS3903339 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. eIF6 was also immunoprecipitated by rabbit anti-eIF6 antibody MBS3903340, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Rabbit Anti-eIF6 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3903339

Other Notes

Small volumes of anti-eIF6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for eIF6 purchase

MBS3903339 is an antibody targeting eIF6. Also known as Rabbit anti-eIF6 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for eIF6. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
eukaryotic translation initiation factor 6 isoform a
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 6
NCBI Official Symbol
EIF6
NCBI Official Synonym Symbols
CAB; EIF3A; eIF-6; p27BBP; ITGB4BP; b(2)gcn; p27(BBP)
UniProt Protein Name
Eukaryotic translation initiation factor 6
UniProt Synonym Protein Names
B(2)GCN homolog; B4 integrin interactor; CAB; p27(BBP)
UniProt Gene Name
EIF6
UniProt Entry Name
IF6_HUMAN
NCBI Protein Information
eukaryotic translation initiation factor 6; B4 integrin interactor; p27 beta-4 integrin-binding protein; eukaryotic translation initiation factor 3A

UniProt Comments for eIF6

EIF6: eukaryotic translation initiation factor 6. Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex.

Protein type: Translation; Translation initiation; Nucleolus

Chromosomal Location of Human Ortholog: 20q12

Cellular Component: nucleoplasm; cytoplasm; nucleolus; lamin filament; nucleus

Molecular Function: protein binding; translation initiation factor activity; ribosome binding; ribosomal large subunit binding

Biological Process: translational initiation; ribosomal large subunit biogenesis and assembly; mature ribosome assembly; ribosome export from nucleus
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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