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ELK1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8119404

Background: E twenty-six (ETS)-like transcription factor 1, also known as Elk1 or Member of ETS oncogene family (ELK1), is a member of the ETS oncogene superfamily which is characterized by a common protein domain that regulates DNA binding to target… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ELK1, Polyclonal Antibody
Full Product Name
Anti-ELK1 Antibody, Rabbit Polyclonal
Product Gene Name
Product Synonym Names
ELK1 Antibody, Rabbit PAb, Antigen Affinity Purified; ELK1, member of ETS oncogene family
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. PBS, pH7.0 with 0.03% Proclin300
Purity/Purification
Protein A & Antigen Affinity
Specificity
Human ELK1
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Immunogen
E Coli-derived Human ELK1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human ELK1 fragment, and purified by antigen affinity chromatography.

Product Categories/Family for anti-ELK1 antibody

Applications Tested/Suitable for anti-ELK1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-ELK1 antibody
WB: 1:500-1:2000
IP: 5-10uL/mg of lysate

Western Blot (WB) of anti-ELK1 antibody

Anti-ELK1 rabbit polyclonal antibody at 1:500 dilution
Lane A: HeLa Whole Cell Lysate
Lane B: HT29 Whole Cell Lysate
Lane C: SW480 Whole Cell Lysate
Lane D: K562 Whole Cell Lysate
Lysates/proteins at 30 ug per lane.
Secondary
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:45 kDa
Observed band size:58 kDa
Rabbit Anti-ELK1 Polyclonal Antibody – Cat# MBS8119404

Immunoprecipitation (IP) of anti-ELK1 antibody

ELK1 was immunoprecipitated using:
Lane A:0.5 mg K562 Whole Cell Lysate
4 uL anti-ELK1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.
Primary antibody:
Anti-ELK1 rabbit polyclonal antibody,at 1:100 dilution
Secondary antibody:
Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size :60 kDa
Rabbit Anti-ELK1 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS8119404

Product References and Citations for anti-ELK1 antibody

Shin SY, et al. (2011) The ETS family transcription factor ELK-1 regulates induction of the cell cycle-regulatory gene p21(Waf1/Cip1) and the BAX gene in sodium arsenite-exposed human keratinocyte HaCaT cells. J Biol Chem. 286 (30): 26860-72.Taniue K, et al. (2011) A member of the ETS family, EHF, and the ATPase RUVBL1 inhibit p53-mediated apoptosis. EMBO Rep. 12 (7): 682-9.

Other Notes

Small volumes of anti-ELK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ELK1 purchase

MBS8119404 is an antibody targeting ELK1. Also known as Anti-ELK1 Antibody, Rabbit Polyclonal. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ELK1. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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