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eNOS Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS624591

Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling and angiogenesis (1,2). eNOS is regulated by phosphorylation… More details

$830 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
eNOS, Polyclonal Antibody
Full Product Name
eNOS, phosphorylated (Thr495) (Endothelial Nitric Oxide Synthase, NOS 3, NOS III)
Product Gene Name
Product Synonym Names
Anti -eNOS, phosphorylated (Thr495) (Endothelial Nitric Oxide Synthase, NOS 3, NOS III)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity/Purification
Affinity Purified
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes endogenous levels of eNOS only when phosphorylated at threonine 495. Species Crossreactivity: Bovine and porcine. Reactivity is expected with human, mouse and rat due to 100% sequence homology with peptide used for immunogen.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
5,517 Da
3D Structure
ModBase 3D Structure for A6H544
Immunogen
Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Thr495 of human eNOS.

Product Categories/Family for anti-eNOS antibody

Applications Tested/Suitable for anti-eNOS antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-eNOS antibody
Suitable for use in Immunoprecipitation and Western Blot.
Dilution: Immunoprecipitation: 1:100
Western Blot: 1:1000

Western Blot (WB) of anti-eNOS antibody

Western blot analysis of cell extracts from porcine aortic endothelial cells (PAECs) 3 days postconfluence treated with 0.3mM H2O2 for 30 minutes or calf intestinal alkaline phosphatase (CIP), using MBS624591 (upper) or control eNOS antibody (lower).
Rabbit Anti-eNOS Polyclonal Antibody – Cat# MBS624591

Other Notes

Small volumes of anti-eNOS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for eNOS purchase

MBS624591 is an antibody targeting eNOS. Also known as eNOS, phosphorylated (Thr495) (Endothelial Nitric Oxide Synthase, NOS 3, NOS III). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for eNOS. It may not necessarily be applicable to this product.

NCBI GI #
UniProt Primary Accession #
NCBI Official Full Name
eNOS protein
UniProt Protein Name
ENOS protein
Protein Family
UniProt Gene Name
eNOS
UniProt Entry Name
A6H544_CALJA

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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