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EPAS1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6295857

This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
EPAS1, Polyclonal Antibody
Full Product Name
EPAS1, NT (EPAS1, BHLHE73, HIF2A, MOP2, PASD2, Endothelial PAS domain-containing protein 1, Basic-helix-loop-helix-PAS protein MOP2, Class E basic helix-loop-helix protein 73, HIF-1-alpha-like factor, Hypoxia-inducible factor 2-alpha, Member of PAS protei
Product Gene Name
Product Synonym Names
EPAS1; BHLHE73; HIF2A; MOP2; PASD2; Endothelial PAS domain-containing protein 1; Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73; HIF-1-alpha-like factor; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; PAS domain-containing protein 2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
96kDa
Chromosome Location
Chromosome: 2; NC_000002.11 (46524541..46613842). Location: 2p21-p16
OMIM
603349
3D Structure
ModBase 3D Structure for Q99814
Immunogen
EPAS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-154 amino acids from the N-terminal region of human EPAS1.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-EPAS1 antibody

Applications Tested/Suitable for anti-EPAS1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-EPAS1 antibody
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-EPAS1 antibody

EPAS1 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the EPAS1 antibody detected the EPAS1 protein (arrow).
Rabbit Anti-EPAS1 Polyclonal Antibody – Cat# MBS6295857

Flow Cytometry (FC/FACS) of anti-EPAS1 antibody

EPAS1 Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-EPAS1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6295857

Product References and Citations for anti-EPAS1 antibody

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Yi, X., et al. Science 329(5987):75-78(2010). Bougatef, F., et al. PLoS ONE 5 (8), E12265 (2010): Hossein Ghaderian, S.M., et al. Pathology 42(5):446-453(2010). Mowat, F.M., et al. PLoS ONE 5 (6), E11103 (2010).

Other Notes

Small volumes of anti-EPAS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EPAS1 purchase

MBS6295857 is an antibody targeting EPAS1. Also known as EPAS1, NT (EPAS1, BHLHE73, HIF2A, MOP2, PASD2, Endothelial PAS domain-containing protein 1, Basic-helix-loop-helix-PAS protein MOP2, Class E basic helix-loop-helix protein 73, HIF-1-alpha-like factor, Hypoxia-inducible factor 2-alpha, Member of PAS protei. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EPAS1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
endothelial PAS domain-containing protein 1
NCBI Official Synonym Full Names
endothelial PAS domain protein 1
NCBI Official Symbol
EPAS1
NCBI Official Synonym Symbols
HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
UniProt Protein Name
Endothelial PAS domain-containing protein 1
UniProt Synonym Protein Names
Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73; bHLHe73; HIF-1-alpha-like factor; HLF; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha; Member of PAS protein 2; PAS domain-containing protein 2
UniProt Gene Name
EPAS1
UniProt Synonym Gene Names
BHLHE73; HIF2A; MOP2; PASD2; EPAS-1; bHLHe73; HLF; HIF-2-alpha; HIF2-alpha
UniProt Entry Name
EPAS1_HUMAN
NCBI Protein Information
endothelial PAS domain-containing protein 1; EPAS-1; HIF2-alpha; HIF-2-alpha; HIF-1alpha-like factor; HIF-1-alpha-like factor; member of PAS protein 2; PAS domain-containing protein 2; hypoxia-inducible factor 2 alpha; hypoxia-inducible factor 2-alpha; ba

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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