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EPRS Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904194

EPRS (glutamyl-prolyl-tRNA synthetase) catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the… More details

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Product Overview

Online Datasheet in PDF
Product Name
EPRS, Polyclonal Antibody
Full Product Name
Rabbit anti-EPRS Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
PIG32; Glutamyl-tRNA synthetase; QPRS; QARS; prolyl-tRNA synthetase; Proline--tRNA ligase; proline-tRNA ligase; proliferation-inducing gene 32 protein; PARS; HLD15; glutamatyl-prolyl-tRNA synthetase; Glutamate--tRNA ligase; bifunctional aminoacyl-tRNA synthetase; glutamate tRNA ligase; glutaminyl-tRNA synthetase; GluRS; GLUPRORS; EARS; cell proliferation-inducing gene 32 protein; bifunctional glutamate/proline--tRNA ligase; proliferation-inducing protein 32; Bifunctional glutamate/proline--tRNA ligase; glutamyl-prolyl-tRNA synthetase
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
170,591 Da
OMIM
CR933648 mRNA
3D Structure
ModBase 3D Structure for P07814
Immunogen
Between 150 and 200
Conjugation
Unconjugated

Product Categories/Family for anti-EPRS antibody

Applications Tested/Suitable for anti-EPRS antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-EPRS antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-EPRS antibody

Detection of human EPRS by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-EPRS (Cat. No. MBS3904194 Lot1, left image, MBS3904195 Lot1, middle image and Cat. No. MBS3904196 Lot1, right image) used at a dilution of 1:1,000 (1ug/ml) Detection: DAB
Rabbit Anti-EPRS Polyclonal Antibody – Cat# MBS3904194

Immunoprecipitation (IP) of anti-EPRS antibody

Detection of human EPRS by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-EPRS antibody MBS3904194 (lot MBS3904194-1) used for IP at 6 ug/mg lysate. EPRS was also immunoprecipitated by rabbit anti-EPRS antibodies MBS3904195 and MBS3904196. For blotting immunoprecipitated EPRS, MBS3904194 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Rabbit Anti-EPRS Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3904194

Western Blot (WB) of anti-EPRS antibody

Detection of human EPRS by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-EPRS antibody MBS3904194 (lot MBS3904194-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-EPRS Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904194

Other Notes

Small volumes of anti-EPRS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EPRS purchase

MBS3904194 is an antibody targeting EPRS. Also known as Rabbit anti-EPRS Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EPRS. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
bifunctional glutamate/proline--tRNA ligase
NCBI Official Synonym Full Names
glutamyl-prolyl-tRNA synthetase
NCBI Official Symbol
EPRS
NCBI Official Synonym Symbols
EARS; PARS; QARS; QPRS; PIG32; GLUPRORS
UniProt Protein Name
Bifunctional glutamate/proline--tRNA ligase
UniProt Synonym Protein Names
Bifunctional aminoacyl-tRNA synthetase; Cell proliferation-inducing gene 32 protein; Glutamatyl-prolyl-tRNA synthetaseIncluding the following 2 domains:Glutamate--tRNA ligase (EC:6.1.1.17)Alternative name(s):Glutamyl-tRNA synthetase; GluRS
UniProt Gene Name
EPRS
UniProt Synonym Gene Names
GLNS; PARS; QARS; QPRS; GluRS
NCBI Protein Information
bifunctional glutamate/proline--tRNA ligase

NCBI Summary for EPRS

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined. [provided by RefSeq, Jul 2008]
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UniProt Comments for EPRS

Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the multisynthetase complex and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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