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EPS8L2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3902282

EPS8L2 (epidermal growth factor receptor kinase substrate 8-like protein 2) bears significant similarity to eps8. Like eps8, EPS8L2 has been shown to interact with SOS-1, a guanine nucleotide exchange factor (GEF), and activate its Rac-GEF activity. EPS8L2… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
EPS8L2, Polyclonal Antibody
Full Product Name
Rabbit anti-EPS8L2 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
DFNB106; epidermal growth factor receptor kinase substrate 8-like protein 2; epidermal growth factor receptor pathway substrate 8-related protein 2; EPS8-like protein 2; EPS8R2; EPS8-related protein 2; Epidermal growth factor receptor kinase substrate 8-like protein 2; EPS8 like 2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
200ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
80,621 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (706120..727727). Location: 11p15.5
OMIM
614988
3D Structure
ModBase 3D Structure for Q9H6S3
Predicted Reactivity
Orangutan
Immunogen
Between 665 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-EPS8L2 antibody

Applications Tested/Suitable for anti-EPS8L2 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-EPS8L2 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-EPS8L2 antibody

Detection of human EPS8L2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPS8L2 antibody A301-84 2 A (lot A301-84 2 A-2) used for IP at 6 ug per reaction. EPS8L2 was also immunoprecipitated by a previous lot of this antibody (lot MBS3902282-1) and rabbit anti-EPS8L2 antibody MBS3902281 For blotting immunoprecipitated EPS8L2, MBS3902281 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-EPS8L2 Polyclonal Antibody – Cat# MBS3902282

Western Blot (WB) of anti-EPS8L2 antibody

Detection of human EPS8L2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EPS8L2 antibody MBS3902282 (lot MBS3902282-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-EPS8L2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3902282

Other Notes

Small volumes of anti-EPS8L2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EPS8L2 purchase

MBS3902282 is an antibody targeting EPS8L2. Also known as Rabbit anti-EPS8L2 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EPS8L2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
epidermal growth factor receptor kinase substrate 8-like protein 2
NCBI Official Synonym Full Names
EPS8-like 2
NCBI Official Symbol
EPS8L2
NCBI Official Synonym Symbols
EPS8R2
UniProt Protein Name
Epidermal growth factor receptor kinase substrate 8-like protein 2
UniProt Synonym Protein Names
Epidermal growth factor receptor pathway substrate 8-related protein 2
UniProt Gene Name
EPS8L2
UniProt Synonym Gene Names
EPS8R2; EPS8-like protein 2
UniProt Entry Name
ES8L2_HUMAN
NCBI Protein Information
epidermal growth factor receptor kinase substrate 8-like protein 2; EPS8-like protein 2; EPS8-related protein 2; epidermal growth factor receptor pathway substrate 8-related protein 2

NCBI Summary for EPS8L2

This gene encodes a member of the EPS8 gene family. The encoded protein, like other members of the family, is thought to link growth factor stimulation to actin organization, generating functional redundancy in the pathways that regulate actin cytoskeletal remodeling. [provided by RefSeq, Dec 2008]

UniProt Comments for EPS8L2

EPS8L2: Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton. Belongs to the EPS8 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, Rac/Rho; Actin-binding

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: protein complex; cytoplasm; plasma membrane; vesicle

Molecular Function: Rho guanyl-nucleotide exchange factor activity; Rac guanyl-nucleotide exchange factor activity; actin binding

Biological Process: regulation of Rho protein signal transduction; Rho protein signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EPS8L2 antibody

 Reference Product  PubMed Publications
 SOS1 antibody  >1 publications with EPS8L2 and SOS1
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