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EPS8L3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6474674

MBS6474674 is an antibody targeting EPS8L3. Also known as EPS8L3, NT (Epidermal Growth Factor Receptor Kinase Substrate 8-like Protein 3, Epidermal Growth Factor Receptor Pathway Substrate 8-related Protein 3, EPS8-like Protein 3, EPS8R3) (AP). Reactive in… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
EPS8L3, Polyclonal Antibody
Full Product Name
EPS8L3, NT (Epidermal Growth Factor Receptor Kinase Substrate 8-like Protein 3, Epidermal Growth Factor Receptor Pathway Substrate 8-related Protein 3, EPS8-like Protein 3, EPS8R3) (AP)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Immunoaffinity chromatography.
Specificity
Recognizes human EPS8L3.
Preparation and Storage
May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
66.861
OMIM
614989
3D Structure
ModBase 3D Structure for Q8TE67
Immunogen
Synthetic peptide selected from the N-terminal region of human EPS8L3 (KLH).
Conjugate
AP
Note
Preservative Free
Applications Tested/Suitable for anti-EPS8L3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-EPS8L3 antibody
WB: 1:100-1:500
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-EPS8L3 antibody

Western blot analysis of EPS8L3 Antibody (N-term) in 293 cell line lysates (35ug/lane). EPS8L3 (arrow) was detected using the purified Pab.
Rabbit Anti-EPS8L3 Polyclonal Antibody – Cat# MBS6474674

Immunohistochemistry (IHC) of anti-EPS8L3 antibody

EPS8L3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EPS8L3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-EPS8L3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6474674

Flow Cytometry (FC/FACS) of anti-EPS8L3 antibody

EPS8L3 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-EPS8L3 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6474674

Product References and Citations for anti-EPS8L3 antibody

1. Tocchetti, A., et.al., Genomics 81(2), 234-244 (2003).

Other Notes

Small volumes of anti-EPS8L3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EPS8L3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Primary Accession #
GenBank Nucleotide #
NCBI Official Full Name
epidermal growth factor receptor kinase substrate 8-like protein 3 isoform b
UniProt Protein Name
Epidermal growth factor receptor kinase substrate 8-like protein 3
UniProt Synonym Protein Names
Epidermal growth factor receptor pathway substrate 8-related protein 3
UniProt Gene Name
EPS8L3
UniProt Synonym Gene Names
EPS8R3; EPS8-like protein 3
UniProt Entry Name
ES8L3_HUMAN

UniProt Comments for EPS8L3

EPS8L3: a protein that is related to epidermal growth factor receptor pathway substrate 8 (EPS8), a substrate for the epidermal growth factor receptor. The function of this protein is unknown. Alternatively spliced transcript variants encoding different isoforms exist. [provided by RefSeq, Jul 2008]

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: cytoplasm
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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