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FASN Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6475694

MBS6475694 is an antibody targeting FASN. Also known as FASN, ID (FAS, Fatty Acid Synthase) (MaxLight 650). Reactive in Human. Host: Rabbit. More details

$1060 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
FASN, Polyclonal Antibody
Full Product Name
FASN, ID (FAS, Fatty Acid Synthase) (MaxLight 650)
Product Gene Name
anti-FASN antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human FASN.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
273,427 Da
OMIM
600212
3D Structure
ModBase 3D Structure for P49327
Immunogen
Synthetic peptide selected from the center region of human FASN (KLH).
Conjugate
MaxLight650
Note
Preservative Free

Product Categories/Family for anti-FASN antibody

Antibodies; Enzymes; Synthase
Applications Tested/Suitable for anti-FASN antibody
Western Blot (WB), FLISA
Application Notes for anti-FASN antibody
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Immunofluorescence (IF) of anti-FASN antibody

Fluorescent confocal image of HepG2 cells stained with FASN (Center) antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP7449c FASN (Center) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the FASN mainly to the mainly to the cytoplasm, supported by Human Protein Atlas Data.
Rabbit Anti-FASN Polyclonal Antibody – Cat# MBS6475694

Western Blot (WB) of anti-FASN antibody

Western blot analysis of FASN Antibody (Center) in Ramos cell line lysates (35ug/lane).FASN (arrow) was detected using the purified Pab.
Rabbit Anti-FASN Polyclonal Antibody, Western Blot (WB) – Cat# MBS6475694

Western Blot (WB) of anti-FASN antibody

Western blot analysis of FASN (arrow) using rabbit polyclonal FASN Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FASN gene (Lane 2) (Origene Technologies).
Rabbit Anti-FASN Polyclonal Antibody, Western Blot (WB) – Cat# MBS6475694

Immunofluorescence (IF) of anti-FASN antibody

Confocal immunofluorescent analysis of FASN Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-FASN Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6475694

Immunofluorescence (IF) of anti-FASN antibody

Confocal immunofluorescent analysis of FASN Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-FASN Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6475694

Product References and Citations for anti-FASN antibody

1. Jayakumar A., Tai M.-H.Proc. Natl. Acad. Sci. USA 92:8695-8699(1995). 2. Kuhajda F.P., Jenner K.Proc. Natl. Acad. Sci. USA 91:6379-6383(1994). 3. Semenkovich C.F., Coleman T.J. Lipid Res. 36:1507-1521(1995).

Other Notes

Small volumes of anti-FASN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FASN. It may not necessarily be applicable to this product.

NCBI GI #
41872631
NCBI GeneID
2194
NCBI Accession #
NP_004095
UniProt Primary Accession #
P49327
UniProt Secondary Accession #
Q13479; Q16702; Q4LE83; Q6P4U5; Q6SS02; Q969R1; Q96C68; Q96IT0
UniProt Related Accession #
P49327
GenBank Nucleotide #
NM_004104
NCBI Official Full Name
fatty acid synthase
NCBI Official Synonym Full Names
fatty acid synthase
NCBI Official Symbol
FASN
NCBI Official Synonym Symbols
FAS; OA-519; SDR27X1
UniProt Protein Name
Fatty acid synthase
Protein Family
Fatty acid synthase
UniProt Gene Name
FASN
UniProt Synonym Gene Names
FAS
UniProt Entry Name
FAS_HUMAN
NCBI Protein Information
fatty acid synthase; short chain dehydrogenase/reductase family 27X, member 1

UniProt Comments for FASN

FASN: a multifunctional enzyme. Catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Protein type: Oxidoreductase; EC 4.2.1.59; EC 2.3.1.39; EC 2.3.1.38; EC 1.3.1.39; Hydrolase; EC 2.3.1.85; EC 1.1.1.100; EC 3.1.2.14; EC 2.3.1.41; Lipid Metabolism - fatty acid biosynthesis; Lyase; Acetyltransferase

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: Golgi apparatus; membrane; mitochondrion; glycogen granule; cytoplasm; plasma membrane; melanosome; cytosol

Molecular Function: fatty-acid synthase activity; protein homodimerization activity; myristoyl-[acyl-carrier-protein] hydrolase activity; 3-oxoacyl-[acyl-carrier-protein] synthase activity; zinc ion binding; enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity; enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity; [acyl-carrier-protein] S-acetyltransferase activity; drug binding; 3-oxoacyl-[acyl-carrier-protein] reductase activity; protein binding; 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; oleoyl-[acyl-carrier-protein] hydrolase activity; [acyl-carrier-protein] S-malonyltransferase activity; palmitoyl-[acyl-carrier-protein] hydrolase activity; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity

Biological Process: osteoblast differentiation; vitamin metabolic process; acetyl-CoA metabolic process; pantothenate metabolic process; positive regulation of cellular metabolic process; energy reserve metabolic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; fatty acid metabolic process; fatty acid biosynthetic process; water-soluble vitamin metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-FASN antibody

 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activation Of Gene Expression By SREBF (SREBP) Pathway antibodies  Activation Of Gene Expression By SREBF (SREBP) Pathway Diagram
 ChREBP Activates Metabolic Gene Expression Pathway antibodies  ChREBP Activates Metabolic Gene Expression Pathway Diagram
 Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective BTD Causes Biotidinase Deficiency Pathway antibodies  Defective BTD Causes Biotidinase Deficiency Pathway Diagram
 Defective CD320 Causes Methylmalonic Aciduria Pathway antibodies  Defective CD320 Causes Methylmalonic Aciduria Pathway Diagram
 Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective GIF Causes Intrinsic Factor Deficiency Pathway antibodies  Defective GIF Causes Intrinsic Factor Deficiency Pathway Diagram

Diseases associated with anti-FASN antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >158 publications with FASN and Neoplasms
 Fatty Liver Antibodies  >81 publications with FASN and Fatty Liver
 Obesity Antibodies  >69 publications with FASN and Obesity
 Liver Diseases Antibodies  >36 publications with FASN and Liver Diseases
 Breast Neoplasms Antibodies  >36 publications with FASN and Breast Neoplasms
 Insulin Resistance Antibodies  >31 publications with FASN and Insulin Resistance
 Inflammation Antibodies  >25 publications with FASN and Inflammation
 Weight Loss Antibodies  >16 publications with FASN and Weight Loss
 Drug Toxicity Antibodies  >15 publications with FASN and Drug Toxicity
 Hepatitis Antibodies  >12 publications with FASN and Hepatitis

Organs/Tissues associated with anti-FASN antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >98 publications with FASN and Liver
 Adipose Tissue Antibodies  >73 publications with FASN and Adipose Tissue
 Blood Antibodies  >72 publications with FASN and Blood
 Connective Tissue Antibodies  >45 publications with FASN and Connective Tissue
 Muscle Antibodies  >34 publications with FASN and Muscle
 Prostate Antibodies  >34 publications with FASN and Prostate
 Mammary Gland Antibodies  >21 publications with FASN and Mammary Gland
 Lung Antibodies  >13 publications with FASN and Lung
 Brain Antibodies  >12 publications with FASN and Brain
 Kidney Antibodies  >10 publications with FASN and Kidney
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