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FAT4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3905978

FAT4 is a member of the protocadherin family, and may play a role in regulating planar cell polarity (PCP). Studies in mice suggest that loss of PCP signaling may cause cystic kidney disease, and mutations in this gene have been associated with Van Maldergem… More details

$250 / 0.01 mg +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
FAT4, Polyclonal Antibody
Full Product Name
Rabbit anti-FAT4 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
FATJ; putative protein product of Nbla00548; protocadherin Fat 4; NBLA00548; HKLLS2; hFat4; FAT-J; VMLDS2; FAT tumor suppressor homolog 4; CDHR11; CDHF14; cadherin-related family member 11; cadherin family member 14; fat-like cadherin protein FAT-J; Protocadherin Fat 4; FAT atypical cadherin 4
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
612411
3D Structure
ModBase 3D Structure for Q6V0I7
Predicted Reactivity
Mouse
Immunogen
Between 4931 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-FAT4 antibody

Applications Tested/Suitable for anti-FAT4 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-FAT4 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-FAT4 antibody

Detection of human FAT4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FAT4 antibody (MBS3905978 lot 1) used for IP at 6 ug per reaction. FAT4 was also immunoprecipitated by rabbit anti-FAT4 antibody BL24449. For blotting immunoprecipitated FAT4, MBS3905978 was used at 0.4 mg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Rabbit Anti-FAT4 Polyclonal Antibody – Cat# MBS3905978

Western Blot (WB) of anti-FAT4 antibody

Detection of human FAT4 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and KM12 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FAT4 antibody (MBS3905978 lot 1) used for WB at 0.4 mg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Rabbit Anti-FAT4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3905978

Other Notes

Small volumes of anti-FAT4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FAT4 purchase

MBS3905978 is an antibody targeting FAT4. Also known as Rabbit anti-FAT4 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FAT4. It may not necessarily be applicable to this product.

NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Protein Name
Protocadherin Fat 4
UniProt Synonym Protein Names
Cadherin family member 14; FAT tumor suppressor homolog 4; Fat-like cadherin protein FAT-J
Protein Family
UniProt Gene Name
FAT4
UniProt Synonym Gene Names
CDHF14; FATJ; hFat4
UniProt Entry Name
FAT4_HUMAN

UniProt Comments for FAT4

FAT4: May function in the regulation of planar cell polarity. Cadherins are cell-cell interaction molecules. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q28.1

Cellular Component: apical part of cell; plasma membrane; integral to membrane

Molecular Function: protein binding; calcium ion binding

Biological Process: heart morphogenesis; heterophilic cell adhesion; inner ear receptor stereocilium organization and biogenesis; plasma membrane organization and biogenesis; neurogenesis; gut development; ureteric bud branching; cerebral cortex development; homophilic cell adhesion

Disease: Van Maldergem Syndrome 2; Hennekam Lymphangiectasia-lymphedema Syndrome 2
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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