• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Fibronectin (FN1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS534416

MBS534416 is an antibody targeting FN1. Also known as Fibronectin antibody (biotin). Reactive in Human fibronectin. Host: Rabbit. Rabbit polyclonal Fibronectin antibody (biotin) conjugated More details

$1700 / 0.1 mg +2 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Fibronectin (FN1), Polyclonal Antibody
Full Product Name
Fibronectin antibody (biotin)
Product Gene Name
anti-FN1 antibody
Product Synonym Names
Polyclonal Fibronectin; Anti-Fibronectin; LETS; CIG; Cold insoluble globulin; Transformation sensitive protein; Migration stimulating factor; MSF
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human fibronectin
Form/Format
Liquid (sterile filtered) in 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 10 mg/ml BSA and 0.01% NaN3
Purity/Purification
Fibronectin antibody (biotin) was purified by affinity chromatography.
Specificity
Human fibronectin
Preparation and Storage
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Molecular Weight
13,341 Da
3D Structure
ModBase 3D Structure for O95608
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Tag/Conjugate
Biotin
Immunogen
Fibronectin antibody was raised in rabbit using fibronectin purified from human plasma as the immunogen.

Product Categories/Family for anti-FN1 antibody

Differentiation & Development; Conjugated Polyclonal Antibodies
Applications Tested/Suitable for anti-FN1 antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-FN1 antibody
ELISA: 1:5,000-1:20,000
IHC: 1:50-1:200
IP: 1:100
WB: 1:500-1:5,000

Immunohistochemistry (IHC) of anti-FN1 antibody

Immunohistochemistry with Fibronectin antibody biotin conjugated at 20X with negative controls (right). Tissue: kidney. Fixation: FFPE buffered formalin 10% conc. Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker (top) or EDTA pH 9.5 Pressure Cooker (bottom). 2ug/ml for 1 hour @ room T. Streptavidin Conjugated HRP 10 ug/ml circa 45 min at room temp. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
Rabbit Anti-FN1 Polyclonal Antibody – Cat# MBS534416

Immunohistochemistry (IHC) of anti-FN1 antibody

Tissue: human kidney at pH9 at 20x and 40x. Fixation: formalin fixed paraffin embedded. Fibronectin antibody at 10 ug/mL for 1 h at RT. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibronectin is cytoplasmic. Staining: Fibronectin as precipitated brown signal (A) with purple nuclear counterstain. With corresponding negative control (B).
Rabbit Anti-FN1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS534416

Immunohistochemistry (IHC) of anti-FN1 antibody

Tissue: human kidney at pH6 at 20x and 40x. Fixation: formalin fixed paraffin embedded. Fibronectin antibody at 10 ug/mL for 1 h at RT. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibronectin is cytoplasmic. Staining: Fibronectin as precipitated brown signal (A) with purple nuclear counterstain. With corresponding negative control (B).
Rabbit Anti-FN1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS534416

Other Notes

Small volumes of anti-FN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FN1. It may not necessarily be applicable to this product.

NCBI GI #
553293
NCBI GeneID
2335
NCBI Accession #
AAA53376.1
UniProt Primary Accession #
O95608
UniProt Related Accession #
P02751
NCBI Official Full Name
fibronectin, partial
NCBI Official Synonym Full Names
fibronectin 1
NCBI Official Symbol
FN1
NCBI Official Synonym Symbols
FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
UniProt Protein Name
Fibronectin
UniProt Synonym Protein Names
Fibronectin
Protein Family
Fibronectin
UniProt Gene Name
FN1
UniProt Entry Name
O95608_HUMAN
NCBI Protein Information
fibronectin

NCBI Summary for FN1

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
Show more

Research Articles on FN1

1. The presence of VN on the material surfaces enhanced cell-mediated FN reorganization and secretion.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-FN1 antibody

 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Fibronectin Matrix Formation Pathway antibodies  Fibronectin Matrix Formation Pathway Diagram

Organs/Tissues associated with anti-FN1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >58 publications with FN1 and Blood
 Lung Antibodies  >33 publications with FN1 and Lung
 Muscle Antibodies  >31 publications with FN1 and Muscle
 Vascular Antibodies  >29 publications with FN1 and Vascular
 Liver Antibodies  >28 publications with FN1 and Liver
 Bone Antibodies  >27 publications with FN1 and Bone
 Kidney Antibodies  >25 publications with FN1 and Kidney
 Brain Antibodies  >21 publications with FN1 and Brain
 Embryonic Tissue Antibodies  >20 publications with FN1 and Embryonic Tissue
 Skin Antibodies  >17 publications with FN1 and Skin
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000