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FNDC5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9148613

This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
FNDC5, Polyclonal Antibody
Full Product Name
FNDC5 Rabbit pAb
Product Gene Name
Product Synonym Names
FRCP2; irisin; FNDC5
Immunogen Sequence
QSPASEPVLF KTPREAEKMA SKNKDEVTMK EMGRNQQLRT GEVLIIVVVL FMWAGVIALF CRQYDIIKDN EPNNNKEKTK SASETSTPEH QGGGLLRSKI
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Purity/Purification
Affinity purification
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Molecular Weight
Calculated MW: 24kDa
Observed MW: 23kDa/
OMIM
611906
3D Structure
ModBase 3D Structure for Q8NAU1
Cross Reactivity
Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 113-212 of human FNDC5 (NP_715637.2-).
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-FNDC5 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-FNDC5 antibody
WB: 1:1000-1:5000
ELISA: Recommended starting concentration is 1ug/mL.
Please optimize the concentration based on your specific assay requirements.

Western Blot (WB) of anti-FNDC5 antibody

Western blot analysis of lysates from Rat heart using FNDC5 Rabbit pAb (MBS9148613) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Rabbit Anti-FNDC5 Polyclonal Antibody – Cat# MBS9148613

Western Blot (WB) of anti-FNDC5 antibody

Western blot analysis of lysates from RD cells using FNDC5 Rabbit pAb (MBS9148613) at 1:1000 dilution incubated overnight at 4?.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Rabbit Anti-FNDC5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9148613

Other Notes

Small volumes of anti-FNDC5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FNDC5 purchase

MBS9148613 is an antibody targeting FNDC5. Also known as FNDC5 Rabbit pAb. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FNDC5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
UniProt Protein Name
Fibronectin type III domain-containing protein 5
UniProt Synonym Protein Names
Fibronectin type III repeat-containing protein 2Irisin
UniProt Gene Name
FNDC5
UniProt Synonym Gene Names
FRCP2
UniProt Entry Name
FNDC5_HUMAN

UniProt Comments for FNDC5

FNDC5: Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: peroxisomal membrane; endoplasmic reticulum; plasma membrane; integral to membrane; extracellular region

Molecular Function: hormone activity

Biological Process: response to muscle activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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