Rabbit Anti-G-CSF Polyclonal Antibody – Cat# MBS695364

G-CSF Antibody – Rabbit Polyclonal

Name: G-CSF Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS695364
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS695364

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hG-CSF. Anti-Human G-CSF specific antibody was purified by affinity chromatography employing immobilized hG-CSF matrix. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

G-CSF, Polyclonal Antibody

Full Product Name

Anti-human G-CSF

Product Gene Name

anti-G-CSF antibody

Product Synonym Names

Anti-Human G-CSF

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Form/Format

Purified

Preparation and Storage

At -20 degree C

Molecular Weight

18,434 Da

OMIM

138970

3D Structure

ModBase 3D Structure for P09919

Immunogen

Recombinant Human G-CSF

Applications Tested/Suitable for anti-G-CSF antibody

Immunohistochemistry (IHC) Formalin/Paraffin, Neutralization, ELISA (EIA), Western Blot (WB)

Application Notes for anti-G-CSF antibody

Immunohistochemistery: This antibody stained formalin-fixed, paraffin-embedded sections of human angiosarcoma. The recommended concentration is 0.25 mug/mL with an overnight incubation at 4 degree C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hG-CSF (0.5 ng/ml), a concentration of 0.04-0.06 ug/ml of this antibody is required.

Sandwich ELISA: To detect hG-CSF by sandwich ELISA (using 100 mul/well antibody solution) a concentration of 0.5 - 2.0 mug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human G-CSF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hG-CSF.

Western Blot: To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Other Notes

Small volumes of anti-G-CSF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for G-CSF purchase

MBS695364 is an antibody targeting G-CSF. Also known as Anti-human G-CSF. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for G-CSF. It may not necessarily be applicable to this product.

UniProt Primary Accession #

P09919

UniProt Secondary Accession #

A8MXR7

UniProt Protein Name

Granulocyte colony-stimulating factor

UniProt Synonym Protein Names

Pluripoietin; INN: Filgrastim; INN: Lenograstim

Protein Family

Granulocyte colony-stimulating factor receptor

UniProt Gene Name

CSF3

UniProt Synonym Gene Names

C17orf33; GCSF; G-CSF

UniProt Entry Name

CSF3_HUMAN

UniProt Comments for G-CSF

G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted, signal peptide; Secreted; Cell cycle regulation

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space

Molecular Function: enzyme binding; growth factor activity; cytokine activity; granulocyte colony-stimulating factor receptor binding

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; granulocyte differentiation; positive regulation of myeloid cell differentiation; positive regulation of protein binding; positive regulation of actin filament polymerization; multicellular organismal development; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-G-CSF antibody

Reference Product	PubMed Publications
CSF3R antibody	>15 publications with G-CSF and CSF3R
MMP9 antibody	>7 publications with G-CSF and MMP9
IL10 antibody	>6 publications with G-CSF and IL10
CXCL12 antibody	>3 publications with G-CSF and CXCL12
ERBB2 antibody	>2 publications with G-CSF and ERBB2
STAT3 antibody	>2 publications with G-CSF and STAT3
SELL antibody	>1 publications with G-CSF and SELL
CXCR4 antibody	>1 publications with G-CSF and CXCR4

Diseases associated with anti-G-CSF antibody

Disease Name	Pubmed Publications
Disease Susceptibility Antibodies	>6 publications with G-CSF and Disease Susceptibility
Nervous System Diseases Antibodies	>5 publications with G-CSF and Nervous System Diseases
Neutropenia Antibodies	>3 publications with G-CSF and Neutropenia
Breast Neoplasms Antibodies	>2 publications with G-CSF and Breast Neoplasms
Leukopenia Antibodies	>2 publications with G-CSF and Leukopenia
Anemia Antibodies	>2 publications with G-CSF and Anemia
Leukemia, Myeloid, Acute Antibodies	>2 publications with G-CSF and Leukemia, Myeloid, Acute
Peripheral Nervous System Diseases Antibodies	>1 publications with G-CSF and Peripheral Nervous System Diseases
Pulmonary Fibrosis Antibodies	>1 publications with G-CSF and Pulmonary Fibrosis
Brain Injuries Antibodies	>1 publications with G-CSF and Brain Injuries

Organs/Tissues associated with anti-G-CSF antibody

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>7 publications with G-CSF and Skin
Lung Antibodies	>5 publications with G-CSF and Lung
Muscle Antibodies	>4 publications with G-CSF and Muscle
Liver Antibodies	>2 publications with G-CSF and Liver
Brain Antibodies	>2 publications with G-CSF and Brain
Uterus Antibodies	>2 publications with G-CSF and Uterus
Uncharacterized Tissue Antibodies	>1 publications with G-CSF and Uncharacterized Tissue